Atenção: Todas as denúncias são sigilosas e sua identidade será preservada.
Os campos nome e e-mail são de preenchimento opcional
Metadados | Descrição | Idioma |
---|---|---|
Autor(es): dc.contributor | Universidade Federal de São Carlos (UFSCar) | - |
Autor(es): dc.contributor | PR | - |
Autor(es): dc.contributor | Universidade Federal de Pernambuco (UFPE) | - |
Autor(es): dc.contributor | Universidade Estadual Paulista (Unesp) | - |
Autor(es): dc.creator | Barbosa, Patrícia | - |
Autor(es): dc.creator | Schemczssen-Graeff, Zelinda | - |
Autor(es): dc.creator | Marques, André | - |
Autor(es): dc.creator | da Silva, Maelin | - |
Autor(es): dc.creator | Favero, Giovani Marino | - |
Autor(es): dc.creator | Sobreiro, Bernardo Passos | - |
Autor(es): dc.creator | de Almeida, Mara Cristina | - |
Autor(es): dc.creator | Moreira-Filho, Orlando | - |
Autor(es): dc.creator | Silva, Duílio Mazzoni Zerbinato de Andrade [UNESP] | - |
Autor(es): dc.creator | Porto-Foresti, Fábio [UNESP] | - |
Autor(es): dc.creator | Foresti, Fausto [UNESP] | - |
Autor(es): dc.creator | Artoni, Roberto Ferreira | - |
Data de aceite: dc.date.accessioned | 2022-02-22T00:54:18Z | - |
Data de disponibilização: dc.date.available | 2022-02-22T00:54:18Z | - |
Data de envio: dc.date.issued | 2021-06-25 | - |
Data de envio: dc.date.issued | 2021-06-25 | - |
Data de envio: dc.date.issued | 2021-05-11 | - |
Fonte completa do material: dc.identifier | http://dx.doi.org/10.3390/cells10051162 | - |
Fonte completa do material: dc.identifier | http://hdl.handle.net/11449/208744 | - |
Fonte: dc.identifier.uri | http://educapes.capes.gov.br/handle/11449/208744 | - |
Descrição: dc.description | The way in which transcriptional activity overcomes the physical DNA structure and gene regulation mechanisms involves complex processes that are not yet fully understood. Modifications in the cytosine-guanine sequence of DNA by 5-mC are preferentially located in heterochromatic regions and are related to gene silencing. Herein, we investigate evidence of epigenetic regulation related to the B chromosome model and transposable elements in A. scabripinnis. Indirect immunofluorescence using anti-5-mC to mark methylated regions was employed along with quantitative ELISA to determine the total genomic DNA methylation level. 5-mC signals were dispersed in the chromosomes of both females and males, with preferential accumulation in the B chromosome. In addition to the heterochromatic methylated regions, our results suggest that methylation is associated with transposable elements (LINE and Tc1-Mariner). Heterochromatin content was measured based on the C-band length in relation to the size of chromosome 1. The B chromosome in A. scabripinnis comprises heterochromatin located in the pericentromeric region of both arms of this isochromosome. In this context, individuals with B chromosomes should have an increased heterochromatin content when compared to individuals that do not. Although, both heterochromatin content and genome methylation showed no significant differences between sexes or in relation to the occurrence of B chromosomes. Our evidence suggests that the B chromosome can have a compensation effect on the heterochromatin content and that methylation possibly operates to silence TEs in A. scabripinnis. This represents a sui generis compensation and gene activity buffering mechanism. | - |
Descrição: dc.description | Department of Genetics and Evolution Federal University of São Carlos, Post Graduate Program in Evolutionary Genetics and Molecular BiologyRodovia Washington Luís Km 235 | - |
Descrição: dc.description | Department of Structural Molecular and Genetic Biology State University of Ponta Grossa PR, Post Graduate Program in Evolutionary BiologyAvenida Carlos Cavalcanti 4748 | - |
Descrição: dc.description | Department of Botany Federal University of Pernambuco PE | - |
Descrição: dc.description | Department of General Biology State University of Ponta Grossa PR, Avenida Carlos Cavalcanti 4748 | - |
Descrição: dc.description | Department of Medicine State University of Ponta Grossa PR, Avenida Carlos Cavalcanti 4748 | - |
Descrição: dc.description | Department of Structural and Functional Biology Institute of Biosciences at Botucatu Sao Paulo State University (UNESP) | - |
Descrição: dc.description | Faculty of Sciences Sao Paulo State University (UNESP) | - |
Descrição: dc.description | Department of Structural and Functional Biology Institute of Biosciences at Botucatu Sao Paulo State University (UNESP) | - |
Descrição: dc.description | Faculty of Sciences Sao Paulo State University (UNESP) | - |
Idioma: dc.language | en | - |
Relação: dc.relation | Cells | - |
???dc.source???: dc.source | Scopus | - |
Palavras-chave: dc.subject | epigenetics | - |
Palavras-chave: dc.subject | isochromosome | - |
Palavras-chave: dc.subject | methylation | - |
Palavras-chave: dc.subject | regulation | - |
Palavras-chave: dc.subject | transposable elements | - |
Título: dc.title | Silencing of Transposable Elements Mediated by 5-mC and Compensation of the Heterochromatin Content by Presence of B Chromosomes in Astyanax scabripinnis | - |
Tipo de arquivo: dc.type | livro digital | - |
Aparece nas coleções: | Repositório Institucional - Unesp |
O Portal eduCAPES é oferecido ao usuário, condicionado à aceitação dos termos, condições e avisos contidos aqui e sem modificações. A CAPES poderá modificar o conteúdo ou formato deste site ou acabar com a sua operação ou suas ferramentas a seu critério único e sem aviso prévio. Ao acessar este portal, você, usuário pessoa física ou jurídica, se declara compreender e aceitar as condições aqui estabelecidas, da seguinte forma: