Optimization of high-solid enzymatic hydrolysis of two-step alkaline and dilute acid-pretreated sugarcane bagasse at low enzyme loadings by response surface methodology

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.creatorIgbojionu, Longinus Ifeanyi [UNESP]-
Autor(es): dc.creatorLaluce, Cecilia [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:54:04Z-
Data de disponibilização: dc.date.available2022-02-22T00:54:04Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2020-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1007/s13399-021-01544-4-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/208676-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/208676-
Descrição: dc.descriptionSugarcane bagasse—a lignocellulosic material derived from sugarcane after crushing and extracting of its juice—is a promising feedstock for bioethanol production. The current study presented a two-step alkaline and acid pretreatment of sugarcane bagasse using sodium hydroxide and maleic acid as a strategy to enhance enzymatic digestibility of sugarcane bagasse. The two-step alkaline and acid pretreatment resulted to a significant increase in glucan content (69.72%) with accompanying decreases in xylan (12.99%) and lignin (4.89%) contents. Preliminary study on enzymatic hydrolysis of the two-step alkaline and acid-pretreated sugarcane bagasse with low solid loading and low cellulase concentrations resulted to high glucose yields. Optimization of high-solid enzymatic hydrolysis of two-step alkaline and acid-pretreated sugarcane bagasse was executed based on Box-Behnken design (BBD). BBD—a response surface methodology—is a powerful method that can be used to test several parameters by applying a minimum number of experimental trials. The optimal level of process variables as indicated by the statistical model namely solid loading (20%, w/w), incubation time (72 h), enzyme loading (2 FPU/g glucan), and substrate feeding (mode 2) were validated. Optimization of the process parameters resulted to 61.85% of glucose yield which was close to its predicted value (65.71%). The current study highlights the potential of high-solid enzymatic hydrolysis using two-step alkaline and acid-pretreated sugarcane bagasse for efficient fermentable sugar production.-
Descrição: dc.descriptionBioenergy Research Institute (IPBEN) Institute of Chemistry São Paulo State University (UNESP), Rua Professor Francisco Degni, 55, Araraquara-
Descrição: dc.descriptionBioenergy Research Institute (IPBEN) Institute of Chemistry São Paulo State University (UNESP), Rua Professor Francisco Degni, 55, Araraquara-
Idioma: dc.languageen-
Relação: dc.relationBiomass Conversion and Biorefinery-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectBioethanol-
Palavras-chave: dc.subjectBox-Behnken design-
Palavras-chave: dc.subjectHigh-solid enzymatic hydrolysis-
Palavras-chave: dc.subjectOptimization-
Palavras-chave: dc.subjectSugarcane bagasse-
Palavras-chave: dc.subjectTwo-step pretreatment-
Título: dc.titleOptimization of high-solid enzymatic hydrolysis of two-step alkaline and dilute acid-pretreated sugarcane bagasse at low enzyme loadings by response surface methodology-
Tipo de arquivo: dc.typelivro digital-
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