Zein based-nanoparticles loaded botanical pesticides in pest control: An enzyme stimuli-responsive approach aiming sustainable agriculture

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUniversidade Federal do ABC (UFABC)-
Autor(es): dc.contributorUniversity of Sorocaba (UNISO)-
Autor(es): dc.creatorMonteiro, Renata Aparecida [UNESP]-
Autor(es): dc.creatorCamara, Marcela Candido [UNESP]-
Autor(es): dc.creatorde Oliveira, Jhones Luiz [UNESP]-
Autor(es): dc.creatorCampos, Estefânia Vangelie Ramos-
Autor(es): dc.creatorCarvalho, Lucas Bragança [UNESP]-
Autor(es): dc.creatorProença, Patrícia Luiza de Freitas [UNESP]-
Autor(es): dc.creatorGuilger-Casagrande, Mariana-
Autor(es): dc.creatorLima, Renata-
Autor(es): dc.creatordo Nascimento, Joacir [UNESP]-
Autor(es): dc.creatorGonçalves, Kelly Cristina [UNESP]-
Autor(es): dc.creatorPolanczyk, Ricardo Antônio [UNESP]-
Autor(es): dc.creatorFraceto, Leonardo Fernandes-
Data de aceite: dc.date.accessioned2022-02-22T00:54:02Z-
Data de disponibilização: dc.date.available2022-02-22T00:54:02Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-09-05-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.jhazmat.2021.126004-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/208662-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/208662-
Descrição: dc.descriptionNanoencapsulation of biopesticides is an important strategy to increase the efficiency of these compounds, reducing losses and adverse effects on non-target organisms. This study describes the preparation and characterisation of zein nanoparticles containing the botanical compounds limonene and carvacrol, responsive to proteolytic enzymes present in the insects guts. The spherical nanoparticles, prepared by the anti-solvent precipitation method, presented in the nanoparticle tracking analysis (NTA) a concentration of 4.7 × 1012 ± 1.3 × 1011 particles.mL−1 and an average size of 125 ± 2 nm. The formulations showed stability over time, in addition to not being phytotoxic to Phaseolus vulgaris plants. In vivo tests demonstrated that formulations of zein nanoparticles containing botanical compounds showed higher mortality to Spodoptera frugiperda larvae. In addition, the FTIC probe (fluorescein isothiocyanate) showed wide distribution in the larvae midgut, as well as being identified in the feces. The trypsin enzyme, as well as the enzymatic extract from insects midgut, was effective in the degradation of nanoparticles containing the mixture of botanical compounds, significantly reducing the concentration of nanoparticles and the changes in size distribution. The zein degradation was confirmed by the disappearance of the protein band in the electrophoresis gel, by the formation of the lower molecular weight fragments and also by the greater release of FTIC after enzymes incubation. In this context, the synthesis of responsive nanoparticles has great potential for application in pest management, increasing the selectivity and specificity of the system and contributing to a more sustainable agriculture.-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionInstitute of Science and Technology São Paulo State University (UNESP)-
Descrição: dc.descriptionHuman and Natural Sciences Center Federal University of ABC (UFABC)-
Descrição: dc.descriptionLaboratory of Bioactivity Assessment and Toxicology of Nanomaterials (LABiToN) University of Sorocaba (UNISO)-
Descrição: dc.descriptionFaculty of Agronomy and Veterinary Sciences São Paulo State University (UNESP)-
Descrição: dc.descriptionInstitute of Science and Technology São Paulo State University (UNESP)-
Descrição: dc.descriptionFaculty of Agronomy and Veterinary Sciences São Paulo State University (UNESP)-
Descrição: dc.descriptionCNPq: #155959/2018-2-
Descrição: dc.descriptionFAPESP: #2017/13328-5-
Descrição: dc.descriptionFAPESP: #2017/21004-5-
Descrição: dc.descriptionFAPESP: #2017/24402-1-
Descrição: dc.descriptionFAPESP: #2018/21142-1-
Descrição: dc.descriptionFAPESP: #2018/23608-8-
Descrição: dc.descriptionFAPESP: #2020/05816-2-
Descrição: dc.descriptionCNPq: #405623/2018-6-
Descrição: dc.descriptionCAPES: 001-
Idioma: dc.languageen-
Relação: dc.relationJournal of Hazardous Materials-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectBiopesticides-
Palavras-chave: dc.subjectCarvacrol-
Palavras-chave: dc.subjectEnzyme-responsive-
Palavras-chave: dc.subjectLimonene-
Palavras-chave: dc.subjectNanoparticles-
Palavras-chave: dc.subjectTrypsin-
Palavras-chave: dc.subjectZein-
Título: dc.titleZein based-nanoparticles loaded botanical pesticides in pest control: An enzyme stimuli-responsive approach aiming sustainable agriculture-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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