Propolis from southeastern Brazil produced by Apis mellifera affects innate immunity by modulating cell marker expression, cytokine production and intracellular pathways in human monocytes

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUniversity of Coimbra-
Autor(es): dc.creatorConte, Fernanda Lopes [UNESP]-
Autor(es): dc.creatorSantiago, Karina Basso [UNESP]-
Autor(es): dc.creatorConti, Bruno José [UNESP]-
Autor(es): dc.creatorCardoso, Eliza de Oliveira [UNESP]-
Autor(es): dc.creatorOliveira, Lucas Pires Garcia [UNESP]-
Autor(es): dc.creatorFeltran, Geórgia da Silva [UNESP]-
Autor(es): dc.creatorZambuzzi, Willian Fernando [UNESP]-
Autor(es): dc.creatorGolim, Marjorie de Assis [UNESP]-
Autor(es): dc.creatorCruz, Maria Teresa-
Autor(es): dc.creatorSforcin, José Maurício [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:53:47Z-
Data de disponibilização: dc.date.available2022-02-22T00:53:47Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-03-04-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1093/jpp/rgaa023-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/208571-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/208571-
Descrição: dc.descriptionOBJECTIVES: Propolis is a bee-made product used for centuries due to its diverse biological properties, including its immunomodulatory action. This work aimed at investigating whether propolis may affect monocyte functions challenged with retinoic acid (RA), B subunit of Escherichia coli heat-labile enterotoxin (EtxB), human melanoma-associated antigen-1 (MAGE-1) and lipopolysaccharide (LPS). METHODS: Monocytes from healthy donors were treated with the stimuli separately or in the presence of propolis. Cell viability was evaluated by MTT assay, cell marker expression was assessed by flow cytometry, cytokine production by ELISA, gene expression by RT-qPCR. KEY FINDINGS: Propolis alone maintained TLR-2, TLR-4, HLA-DR, CD40 and CD80 expression in the monocytes; however, its combination with either MAGE-1 or LPS decreased CD40 expression triggered by the stimuli. Propolis maintained RA action on cell marker expression. Propolis inhibited TNF-α (with either EtxB or MAGE-1) and IL-6 (with either RA or MAGE-1), and increased IL-10 (with MAGE-1) production. Propolis downmodulated LC3 expression induced by LPS. It also induced a lower NF-kB expression than control cells and its combination with RA induced a higher expression than the stimulus alone. CONCLUSIONS: Propolis potentially affected innate immunity by downmodulating the monocytes pro-inflammatory activity.-
Descrição: dc.descriptionDepartment of Chemical and Biological Sciences Institute of Biosciences São Paulo State University (UNESP)-
Descrição: dc.descriptionBotucatu Blood Center School of Medicine São Paulo State University (UNESP)-
Descrição: dc.descriptionCenter for Neurosciences and Cellular Biology Faculty of Pharmacy University of Coimbra-
Descrição: dc.descriptionDepartment of Chemical and Biological Sciences Institute of Biosciences São Paulo State University (UNESP)-
Descrição: dc.descriptionBotucatu Blood Center School of Medicine São Paulo State University (UNESP)-
Formato: dc.format135-144-
Idioma: dc.languageen-
Relação: dc.relationThe Journal of pharmacy and pharmacology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjecthuman monocytes-
Palavras-chave: dc.subjectimmunomodulation-
Palavras-chave: dc.subjectinflammation-
Palavras-chave: dc.subjectinnate immunity-
Palavras-chave: dc.subjectpropolis-
Título: dc.titlePropolis from southeastern Brazil produced by Apis mellifera affects innate immunity by modulating cell marker expression, cytokine production and intracellular pathways in human monocytes-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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