Development of a novel biosensor for Creatine Kinase (CK-MB) using Surface Plasmon Resonance (SPR)

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Autor(es): dc.contributorUniversidade Federal de São Carlos (UFSCar)-
Autor(es): dc.contributorUniversidade Estadual de Campinas (UNICAMP)-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.creatorFerreira, André L.-
Autor(es): dc.creatorde Lima, Lucas F.-
Autor(es): dc.creatorMoraes, Ariana S.-
Autor(es): dc.creatorRubira, Rafael J.G. [UNESP]-
Autor(es): dc.creatorConstantino, Carlos J.L. [UNESP]-
Autor(es): dc.creatorLeite, Fabio L.-
Autor(es): dc.creatorDelgado-Silva, Adriana O.-
Autor(es): dc.creatorFerreira, Marystela-
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Descrição: dc.descriptionIn this paper, we report a new biosensor for detection of the cardiac biomarker Creatine Kinase (CK-MB) using Surface Plasmon Surface (SPR) technique, with the purpose of the diagnosis of Acute Myocardial Infarction (AMI). For this, we employed gold nanoparticles (AuNP) stabilized with Cysteamine (Cys), and functionalized with Creatine Phosphate (Pcrea), which promote an increase in the sensitivity and accuracy for creatine kinase (CK-MB) detection in saliva and urine samples. The characteristics of the electronic transfer of the sensor could be observed by Electrochemical Impedance Spectroscopy (EIS) and Cyclic Voltammetry (CV), that showed significant redox peaks on electrode surface evidencing the high electron exchange process and low resistance charge transfer between the electrolyte solution and the modified surface. The morphological characteristics were confirmed by Atomic Force Microscopy (AFM), micro-Raman and Contact Angle (CA), as well as the adsorption and interaction processes between the materials, which could be observed by Surface Plasmon Resonance (SPR). The SPR analytical curve, ranged from 5.0 ng mL−1 to 100.0 ng mL−1, provided some important parameters, such as limit of detection (LOD) of 0.209 ng mL−1, limit of quantification (LOQ) of 0.696 ng mL−1, and sensitivity of 8.67 × 10–3 ± 0.17 × 10–3 (°) mL ng−1. The specific interaction between functionalized substrate and CK-MB demonstrates a high affinity of 15.14 ng mL−1 based on the Michaelis-Menten fitting.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionCenter of Science and Technology for Sustainability (CCTS) Federal University of São Carlos (UFSCar)-
Descrição: dc.descriptionDepartment of Analytical Chemistry Institute of Chemistry State University of Campinas – UNICAMP, P.O. Box 6154-
Descrição: dc.descriptionSão Paulo State University Júlio de Mesquita Filho (UNESP)-
Descrição: dc.descriptionSão Paulo State University Júlio de Mesquita Filho (UNESP)-
Descrição: dc.descriptionFAPESP: 2016/19387-0-
Descrição: dc.descriptionFAPESP: 2018/04061-8-
Descrição: dc.descriptionFAPESP: 2019/02692-3-
Idioma: dc.languageen-
Relação: dc.relationApplied Surface Science-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAcutemyocardial infarction-
Palavras-chave: dc.subjectCardiac biomarker Creatine Kinase (CK-MB)-
Palavras-chave: dc.subjectMicro-Raman-
Palavras-chave: dc.subjectOptical biosensor-
Palavras-chave: dc.subjectSelf-assembly-
Palavras-chave: dc.subjectSurface Plasmon Surface (SPR)-
Título: dc.titleDevelopment of a novel biosensor for Creatine Kinase (CK-MB) using Surface Plasmon Resonance (SPR)-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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