Screening of Saccharum spp. genotypes for sugarcane yellow leaf virus resistance by combining symptom phenotyping and highly precise virus titration

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorCentro de Sanidade Vegetal-
Autor(es): dc.contributorCentro de Cana-
Autor(es): dc.contributorEscuela Superior Politécnica Del Litoral-
Autor(es): dc.creatorBurbano, Roberto C.V. [UNESP]-
Autor(es): dc.creatorGonçalves, Marcos C.-
Autor(es): dc.creatorNobile, Paula M.-
Autor(es): dc.creatordos Anjos, Ivan A.-
Autor(es): dc.creatorda Silva, Marcel F.-
Autor(es): dc.creatorPerecin, Dilermando [UNESP]-
Autor(es): dc.creatorAnjos, Luciana O.S.-
Autor(es): dc.creatorFernandes, Vivian B.V.R.-
Autor(es): dc.creatorPinto, Luciana R.-
Data de aceite: dc.date.accessioned2022-02-22T00:53:26Z-
Data de disponibilização: dc.date.available2022-02-22T00:53:26Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.cropro.2021.105577-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/208447-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/208447-
Descrição: dc.descriptionYellow leaf disease (YLD), caused by sugarcane yellow leaf virus (SCYLV), has been reported to infect sugarcane worldwide causing significant yield losses and is considered a major disease of this crop. A panel composed of 98 genotypes encompassing basic germplasm, commercial cultivars, and elite clones was assayed in the nursery and a replicated field for resistance to SCYLV, combining symptom expression and virus quantification. Virus symptom intensity was evaluated using a diagrammatic scale while virus titer was estimated by DAS-ELISA (Double Antibody Sandwich-ELISA) and RT-qPCR (Reverse Transcription Quantitative PCR) in the nursery and field trials, respectively. Resistance was evaluated alongside symptom development kinetics. Based on the symptom intensity, 52 (53.06%) genotypes were classified as resistant in the nursery and 42 (42.86%) in the field. Twenty-nine (29.59%) genotypes showed no symptoms in the nursery and field trial. Moderately resistant genotypes showed a low correlation between virus titer and symptom intensity. The kinetics of symptom development increased over time in moderately susceptible and susceptible genotypes. The SCYLV incidence assessed by RT-qPCR was 92.55%, which was detected in 83% of the asymptomatic genotypes. The broad-sense heritability based on symptom expression and relative quantification was 52.62% and 68%, respectively. The virus quantification assays allowed for the identification of potential genotypes immune to SCYLV.-
Descrição: dc.descriptionSecretaría de Educación Superior, Ciencia, Tecnología e Innovación-
Descrição: dc.descriptionUniversidade Estadual Paulista UNESP, Jaboticabal-
Descrição: dc.descriptionInstituto Biológico Centro de Sanidade Vegetal, São Paulo-
Descrição: dc.descriptionInstituto Agronômico Centro de Cana, Ribeirão Preto-
Descrição: dc.descriptionEscuela Superior Politécnica Del Litoral-
Descrição: dc.descriptionUniversidade Estadual Paulista UNESP, Jaboticabal-
Idioma: dc.languageen-
Relação: dc.relationCrop Protection-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectDAS-ELISA-
Palavras-chave: dc.subjectPhenotyping-
Palavras-chave: dc.subjectPolerovirus-
Palavras-chave: dc.subjectRT-qPCR-
Palavras-chave: dc.subjectSugarcane-
Palavras-chave: dc.subjectYellow leaf disease-
Título: dc.titleScreening of Saccharum spp. genotypes for sugarcane yellow leaf virus resistance by combining symptom phenotyping and highly precise virus titration-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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