Anti-biofilm activity and in vitro biocompatibility of copper surface prepared by cold gas spray

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorBarcelona University-
Autor(es): dc.creatorda Silva, F. S. [UNESP]-
Autor(es): dc.creatorde Paula e Silva, A. C.A. [UNESP]-
Autor(es): dc.creatorBarbugli, P. A. [UNESP]-
Autor(es): dc.creatorCinca, N.-
Autor(es): dc.creatorDosta, S.-
Autor(es): dc.creatorCano, I. G.-
Autor(es): dc.creatorGuilemany, J. M.-
Autor(es): dc.creatorVergani, C. E. [UNESP]-
Autor(es): dc.creatorBenedetti, A. V. [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:53:25Z-
Data de disponibilização: dc.date.available2022-02-22T00:53:25Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-04-15-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.surfcoat.2021.126981-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/208439-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/208439-
Descrição: dc.descriptionIn this work the cold gas spray (CGS) technique was used to produce anti-biofilm and biocompatibility copper surfaces. Copper was deposited onto carbon steel and the phase composition, morphology, porosity, and adherence of the coating were investigated. Methicillin-susceptible Staphylococcus aureus (MSSA-ATCC 25923), Escherichia coli (ATCC 25922) and Candida albicans (SC 5314) biofilms were used in the antimicrobial tests, and the biocompatibility of the coatings was tested in human monocytes (THP-1 cell line). The main physical characteristics of the copper coating were thickness (640 ± 5) μm, porosity 0.4% and adherence 30 MPa. The X-ray diffraction pattern of the coatings showed only crystalline phase of copper. The plate spreading method, XTT assay, confocal fluorescence microscopy and scanning electron microscopy images showed that copper coatings were effective as an antimicrobial agent by blocking the adhesion and viability of C. albicans and killing the surface adhered bacterial strains, significantly reducing the biofilm survival. The viability rate of THP-1 cells for 24 h was 95% and the confocal microscopy showed the same labeling as the standard culture conditions.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionUNESP - São Paulo State University Institute of Chemistry-
Descrição: dc.descriptionThermal Spray Center Barcelona University-
Descrição: dc.descriptionUNESP - São Paulo State University – Araraquara School of Dentistry-
Descrição: dc.descriptionUNESP - São Paulo State University Institute of Chemistry-
Descrição: dc.descriptionUNESP - São Paulo State University – Araraquara School of Dentistry-
Descrição: dc.descriptionCNPq: 153177/2014-4-
Descrição: dc.descriptionCNPq: 201325/2014-4-
Descrição: dc.descriptionCNPq: 431895/2016-3-
Idioma: dc.languageen-
Relação: dc.relationSurface and Coatings Technology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAnti-biofilm surface-
Palavras-chave: dc.subjectCandida albicans-
Palavras-chave: dc.subjectCold gas spray-
Palavras-chave: dc.subjectCopper-
Palavras-chave: dc.subjectEscherichia coli-
Palavras-chave: dc.subjectStaphylococcus aureus-
Título: dc.titleAnti-biofilm activity and in vitro biocompatibility of copper surface prepared by cold gas spray-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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