Erythrina velutina Willd. alkaloids: Piecing biosynthesis together from transcriptome analysis and metabolite profiling of seeds and leaves

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorFederal University of Rio Grande do Norte (UFRN)-
Autor(es): dc.contributorRural Federal University of the Semiarid-
Autor(es): dc.contributorInstitute for Sustainable Development and Environment-
Autor(es): dc.contributorUniversity of Florence-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorFederal University of Rio Grande do Sul-
Autor(es): dc.contributorBarcelona Institute of Science and Technology-
Autor(es): dc.contributorFederal University of Rio Grande do Norte-
Autor(es): dc.contributorFederal University of Rio de Janeiro-
Autor(es): dc.contributorBrazilian Center for Research in Energy and Materials (CNPEM)-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.creatorChacon, Daisy Sotero-
Autor(es): dc.creatorTorres, Taffarel Melo-
Autor(es): dc.creatorda Silva, Ivanice Bezerra-
Autor(es): dc.creatorde Araújo, Thiago Ferreira-
Autor(es): dc.creatorRoque, Alan de Araújo-
Autor(es): dc.creatorPinheiro, Francisco Ayrton Senna Domingos-
Autor(es): dc.creatorSelegato, Denise-
Autor(es): dc.creatorPilon, Alan-
Autor(es): dc.creatorReginaldo, Fernanda Priscila Santos-
Autor(es): dc.creatorda Costa, Cibele Tesser-
Autor(es): dc.creatorVilasboa, Johnatan-
Autor(es): dc.creatorFreire, Rafael Teixeira-
Autor(es): dc.creatorVoigt, Eduardo Luiz-
Autor(es): dc.creatorZuanazzi, José Angelo Silveira-
Autor(es): dc.creatorLibonati, Renata-
Autor(es): dc.creatorRodrigues, Julia Abrantes-
Autor(es): dc.creatorSantos, Filippe Lemos Maia-
Autor(es): dc.creatorScortecci, Kátia Castanho-
Autor(es): dc.creatorLopes, Norberto Peporine-
Autor(es): dc.creatorFerreira, Leandro De Santis-
Autor(es): dc.creatordos Santos, Leandro Vieira-
Autor(es): dc.creatorCavalheiro, Alberto José [UNESP]-
Autor(es): dc.creatorFett-Neto, Arthur Germano-
Autor(es): dc.creatorGiordani, Raquel Brandt-
Data de aceite: dc.date.accessioned2022-02-22T00:53:23Z-
Data de disponibilização: dc.date.available2022-02-22T00:53:23Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2020-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.jare.2021.01.017-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/208426-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/208426-
Descrição: dc.descriptionIntroduction: Natural products of pharmaceutical interest often do not reach the drug market due to the associated low yields and difficult extraction. Knowledge of biosynthetic pathways is a key element in the development of biotechnological strategies for plant specialized metabolite production. The scarce studies regarding non-model plants impair advances in this field. Erythrina spp. are mainly used as central nervous system depressants in folk medicine and are important sources of bioactive tetracyclic benzylisoquinoline alkaloids, which can act on several pathology-related biological targets. Objective: Herein the purpose is to employ combined transcriptome and metabolome analyses (seeds and leaves) of a non-model medicinal Fabaceae species grown in its unique arid natural habitat. The study tries to propose a putative biosynthetic pathway for the bioactive alkaloids by using an omic integrated approach. Methods: The Next Generation Sequencing-based transcriptome (de novo RNA sequencing) was carried out in a Illumina NextSeq 500 platform. Regarding the targeted metabolite profiling, Nuclear Magnetic Resonance and the High-Performance Liquid Chromatography coupled to a micrOTOF-QII, High Resolution Mass Spectrometer, were used. Results: This detailed macro and micromolecular approach applied to seeds and leaves of E. velutina revealed 42 alkaloids by metabolome tools. Based on the combined evidence, 24 gene candidates were put together in a putative pathway leading to the singular alkaloid diversity of this species. Conclusion: These results contribute by indicating potential biotechnological targets Erythrina alkaloids biosynthesis as well as to improve molecular databases with omic data from a non-model medicinal plant. Furthermore, they reveal an interesting chemical diversity in Erythrina velutina harvested in Caatinga. Last, but not least, this data may also contribute to tap Brazilian biodiversity in a rational and sustainable fashion, promoting adequate public policies for preservation and protection of sensitive areas within the Caatinga.-
Descrição: dc.descriptionDepartment of Pharmacy Federal University of Rio Grande do Norte (UFRN)-
Descrição: dc.descriptionBioinformatics Biostatistics and Computer Biology Nucleus Rural Federal University of the Semiarid-
Descrição: dc.descriptionInstitute for Sustainable Development and Environment, Dunas Park Herbarium-
Descrição: dc.descriptionMagnetic Resonance Center (CERM) University of Florence-
Descrição: dc.descriptionFaculty of Pharmaceutical Sciences of Ribeirão Preto University of São Paulo (FCFRP-USP)-
Descrição: dc.descriptionPlant Physiology Laboratory Center for Biotechnology and Department of Botany Federal University of Rio Grande do Sul-
Descrição: dc.descriptionSignal and Information Processing for Sensing Systems Institute for Bioengineering of Catalonia (IBEC) Barcelona Institute of Science and Technology-
Descrição: dc.descriptionDepartment of Cell Biology and Genetics Center for Biosciences Federal University of Rio Grande do Norte-
Descrição: dc.descriptionLaboratory of Pharmacognosy Federal University of Rio Grande do Sul-
Descrição: dc.descriptionDepartment of Meteorology Institute of Geoscience Federal University of Rio de Janeiro-
Descrição: dc.descriptionBrazilian Bioethanol Science and Technology Laboratory Brazilian Center for Research in Energy and Materials (CNPEM)-
Descrição: dc.descriptionChemistry Institute São Paulo State University (UNESP)-
Descrição: dc.descriptionChemistry Institute São Paulo State University (UNESP)-
Idioma: dc.languageen-
Relação: dc.relationJournal of Advanced Research-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectBenzylisoquinoline Alkaloids-
Palavras-chave: dc.subjectCaatinga-
Palavras-chave: dc.subjectErythrina velutina-
Palavras-chave: dc.subjectTargeted metabolite profile-
Palavras-chave: dc.subjectTranscriptome-
Título: dc.titleErythrina velutina Willd. alkaloids: Piecing biosynthesis together from transcriptome analysis and metabolite profiling of seeds and leaves-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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