Exploring TERRA during Leishmania major developmental cycle and continuous in vitro passages

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUniversity of Leeds-
Autor(es): dc.contributorUniversidade Estadual de Campinas (UNICAMP)-
Autor(es): dc.contributorUniversity of Washington-
Autor(es): dc.contributorInstitute of Molecular Medicine-
Autor(es): dc.creatorMorea, Edna Gicela Ortiz [UNESP]-
Autor(es): dc.creatorVasconcelos, Elton Jose Rosas-
Autor(es): dc.creatorAlves, Cristiane de Santis [UNESP]-
Autor(es): dc.creatorGiorgio, Selma-
Autor(es): dc.creatorMyler, Peter J.-
Autor(es): dc.creatorLangoni, Helio [UNESP]-
Autor(es): dc.creatorAzzalin, Claus Maria-
Autor(es): dc.creatorCano, Maria Isabel Nogueira [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:53:18Z-
Data de disponibilização: dc.date.available2022-02-22T00:53:18Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-03-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.ijbiomac.2021.01.192-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/208390-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/208390-
Descrição: dc.descriptionTelomeres from different eukaryotes, including trypanosomatids, are transcribed into TERRA noncoding RNAs, crucial in regulating chromatin deposition and telomere length. TERRA is transcribed from the C-rich subtelomeric strand towards the 3′-ends of the telomeric array. Using bioinformatics, we confirmed the presence of subtelomeric splice acceptor sites at all L. major chromosome ends. Splice leader sequences positioned 5′ upstream of L. major chromosomes subtelomeres were then mapped using SL-RNA-Seq libraries constructed from three independent parasite life stages and helped confirm TERRA expression from several chromosomes ends. Northern blots and RT-qPCR validated the results showing that L. major TERRA is processed by trans-splicing and polyadenylation coupled reactions. The number of transcripts varied with the parasite's life stage and continuous passages, being more abundant in the infective forms. However, no putative subtelomeric promoters involved in TERRA's transcriptional regulation were detected. In contrast, the observed changes in parasite's telomere length during development, suggest that differences in telomeric base J levels may control TERRA transcription in L. major. Also, TERRA-R loops' detection, mainly in the infective forms, was suggestive of TERRA's involvement in telomere protection. Therefore, Leishmania TERRA shares conserved features with other eukaryotes and advances new telomere specific functions in a Public Health-impacting parasite.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionDepartment of Chemical and Biological Sciences Biosciences Institute São Paulo State University UNESP-
Descrição: dc.descriptionLeeds Omics University of Leeds-
Descrição: dc.descriptionDepartment of Animal Biology Biology Institute State University of Campinas UNICAMP-
Descrição: dc.descriptionDepartment of Global Health and Department of Biomedical Informatics & Medical Education University of Washington-
Descrição: dc.descriptionDepartment of Public Health Veterinary Medical School São Paulo State University UNESP-
Descrição: dc.descriptionInstitute of Molecular Medicine-
Descrição: dc.descriptionDepartment of Chemical and Biological Sciences Biosciences Institute São Paulo State University UNESP-
Descrição: dc.descriptionDepartment of Public Health Veterinary Medical School São Paulo State University UNESP-
Descrição: dc.descriptionFAPESP: 2018/04375-2-
Descrição: dc.descriptionFAPESP: 2018/23302-6-
Descrição: dc.descriptionCNPq: 405581/2018-1-
Formato: dc.format573-586-
Idioma: dc.languageen-
Relação: dc.relationInternational Journal of Biological Macromolecules-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectLeishmania sp. life stages and in vitro passages-
Palavras-chave: dc.subjectTelomere transcription-
Palavras-chave: dc.subjectTERRA R-loops-
Título: dc.titleExploring TERRA during Leishmania major developmental cycle and continuous in vitro passages-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

Não existem arquivos associados a este item.