Bone repair assessment of critical size defects in rats treated with mineralized bovine bone (Bio-Oss®) and photobiomodulation therapy: a histomorphometric and immunohistochemical study

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Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.creatorTorquato, Letícia Cavassini [UNESP]-
Autor(es): dc.creatorSuárez, Eduardo Antonio Chelin [UNESP]-
Autor(es): dc.creatorBernardo, Daniella Viscensotto [UNESP]-
Autor(es): dc.creatorPinto, Isis Luzcybel Ribeiro [UNESP]-
Autor(es): dc.creatorMantovani, Ludmilla Oliveira [UNESP]-
Autor(es): dc.creatorSilva, Thiago Igor Lemes [UNESP]-
Autor(es): dc.creatorJardini, Maria Aparecida Neves [UNESP]-
Autor(es): dc.creatorSantamaria, Mauro Pedrine [UNESP]-
Autor(es): dc.creatorDe Marco, Andrea Carvalho [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:53:01Z-
Data de disponibilização: dc.date.available2022-02-22T00:53:01Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2020-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1007/s10103-020-03234-5-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/208296-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/208296-
Descrição: dc.descriptionThis study aimed to investigate the effects of administering photobiomodulation therapy (PBM) with bovine bone matrix on critical size defects in rats. Seventy-two adult male rats (albinus, Wistar), 90 days old, were used. Defect of 5 mm in diameter was made in their calvaria. The animals were divided into 4 groups: C-blood clot, B-Bio-Oss®, L-PBM, B+L-Bio-Oss®+PBM. Each group has been subdivided into 07, 30, and 60 days of observation. For PBM, a low GaAlAs energy of 660 nm was irradiated, total energy density of 45 J/cm2. PBM was conducted in a trans-surgical form once only. For immunohistochemistry, a semi-quantitative analysis was made of expression of osteoprotegerin (OPG), nuclear kappa B-factor ligand receptor activator (RANKL), and tartrate-resistant acid phosphatase (TRAP). All histomorphometric data were statistically analyzed by ANOVA and Tukey test, significance level of 5%. The groups that showed the highest proportion of neoformation were L (0.39% ± 0.13) and C (0.37% ± 0.97), but groups B and B+L had larger defect size (C-1.75 mm2 ± 0.40, B-3.02 mm2 ± 0.63, L-2.45 mm2 ± 0.53, B+L-3.23 mm2 ± 1.01). In immunohistochemistry, groups B and B+L had higher immunostaining scores for OPG and RANKL at 60 days, and TRAP immunostaining increased in all groups at 30 days, but group L was the only one to present specimens with score 0. Although, at 60 days, groups L and C presented the highest proportion of bone neoformation, at 30 days group B+L had more than twice as much bone neoformation as group B, the choice of treatment application should depend on the aim of the treatment.-
Descrição: dc.descriptionDepartment of Diagnosis and Surgery São Paulo State University (UNESP) Institute of Science and Technology, Av. Eng. Francisco José Longo, n° 777 - Jardim São Dimas-
Descrição: dc.descriptionSão Paulo State University (UNESP) Institute of Science and Technology-
Descrição: dc.descriptionDepartment of Diagnosis and Surgery – Division of Periodontology São Paulo State University (UNESP) Institute of Science and Tecnology-
Descrição: dc.descriptionDepartment of Diagnosis and Surgery São Paulo State University (UNESP) Institute of Science and Technology, Av. Eng. Francisco José Longo, n° 777 - Jardim São Dimas-
Descrição: dc.descriptionSão Paulo State University (UNESP) Institute of Science and Technology-
Descrição: dc.descriptionDepartment of Diagnosis and Surgery – Division of Periodontology São Paulo State University (UNESP) Institute of Science and Tecnology-
Idioma: dc.languageen-
Relação: dc.relationLasers in Medical Science-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectBiocompatible materials-
Palavras-chave: dc.subjectBone regeneration-
Palavras-chave: dc.subjectBone transplantation-
Palavras-chave: dc.subjectImmunohistochemistry-
Palavras-chave: dc.subjectLow-level light therapy-
Título: dc.titleBone repair assessment of critical size defects in rats treated with mineralized bovine bone (Bio-Oss®) and photobiomodulation therapy: a histomorphometric and immunohistochemical study-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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