Association of hyaluronic acid with a deproteinized bovine graft improves bone repair and increases bone formation in critical-size bone defects

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Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorPrivate practice. Director of the Pro-clinic Nucleus of Orofacial Harmonization-
Autor(es): dc.creatorMatheus, Henrique R [UNESP]-
Autor(es): dc.creatorErvolino, Edilson [UNESP]-
Autor(es): dc.creatorGusman, David Jonathan Rodrigues [UNESP]-
Autor(es): dc.creatorAlves, Breno Edson Sendão [UNESP]-
Autor(es): dc.creatorFiorin, Luiz Guilherme [UNESP]-
Autor(es): dc.creatorPereira, Priscilla Aparecida-
Autor(es): dc.creatorde Almeida, Juliano Milanezi [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:52:51Z-
Data de disponibilização: dc.date.available2022-02-22T00:52:51Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2019-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1002/JPER.20-0613-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/208252-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/208252-
Descrição: dc.descriptionBackground: This study is designed to evaluate the potential of different formulations of hyaluronic acid (HA) to improve new bone formation in critical-size calvaria defect (CSD) when combined with a deproteinized bovine graft (DBG) material. Methods: Thirty male rats were used. A 5-mm-diameter CSD was created and three experimental groups (n = 10) were randomly assigned based on the treatments performed. Group DBG: CSD filled with a DBG; group DBG/LV: CSD filled by the combination of DBG and HA in a low-viscosity crosslinking agent; group DBG/HV: CSD filled by the combination of DBG and HA in a high-viscosity crosslinking agent. Animals were euthanized 30 days postoperatively. Histological, histometric (percentage of newly formed bone [PNFB], percentage of remaining graft particles, histochemical, and immunohistochemical (bone morphogenetic protein 2/4 [BMP2/4], osteocalcin [OCN], and tartrate-resistant acid phosphatase [TRAP]) analyses were performed. Results: The highest PNFB was observed in DBG/HV when compared with the other groups (P ≤0.05). DBG/LV and DBG/HV presented almost no inflammatory cells. In contrast, inflammation was observed in group DBG. Extensive resorption of graft particles was observed in group DBG, which was not present in DBG/LV and DBG/HV as confirmed by the larger size of the particles (P ≤0.05). BMP2/4 and OCN immunolabeling were higher in DBG/HV when compared with group DBG (P ≤0.05). Increased number of TRAP-positive cells was observed in DBG/LV and DBG/HV (P ≤0.05). Lower percentage of mature collagen fibers was observed in DBG/HV (P ≤0.05). Conclusion: The combination of HA in a high-viscosity crosslinking agent with DBG improves the bone repair process and increases the amount of newly formed bone towards CSDs in rat calvaria.-
Descrição: dc.descriptionDepartment of Diagnosis and Surgery‒Periodontics Division School of Dentistry São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Basic Science School of Dentistry São Paulo State University (UNESP)-
Descrição: dc.descriptionPrivate practice. Director of the Pro-clinic Nucleus of Orofacial Harmonization-
Descrição: dc.descriptionDepartment of Diagnosis and Surgery‒Periodontics Division School of Dentistry São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Basic Science School of Dentistry São Paulo State University (UNESP)-
Idioma: dc.languageen-
Relação: dc.relationJournal of Periodontology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectanimal experimentation-
Palavras-chave: dc.subjectbiomarkers-
Palavras-chave: dc.subjectbone-
Palavras-chave: dc.subjectbone regeneration-
Palavras-chave: dc.subjectbone transplantation-
Título: dc.titleAssociation of hyaluronic acid with a deproteinized bovine graft improves bone repair and increases bone formation in critical-size bone defects-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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