Bothrops pauloensis snake venom-derived Asp-49 and Lys-49 phospholipases A2 mediates acute kidney injury by oxidative stress and release of inflammatory cytokines

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorFederal University of Ceara-
Autor(es): dc.contributorUniversidade Federal de São Paulo (UNIFESP)-
Autor(es): dc.contributorState University of Ceara-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.creatorMarinho, Aline Diogo-
Autor(es): dc.creatorSilveira, João Alison de Moraes-
Autor(es): dc.creatorChaves Filho, Adriano José Maia-
Autor(es): dc.creatorJorge, Antônio Rafael Coelho-
Autor(es): dc.creatorNogueira Júnior, Francisco Assis-
Autor(es): dc.creatorPereira, Venúcia Bruna Magalhães-
Autor(es): dc.creatorde Aquino, Pedro Everson Alexandre-
Autor(es): dc.creatorPereira, Cássia Arruda Souza-
Autor(es): dc.creatorEvangelista, Janaina Serra Azul Monteiro-
Autor(es): dc.creatorMacedo, Danielle Silveira-
Autor(es): dc.creatorLima Júnior, Roberto César Pereira-
Autor(es): dc.creatorToyama, Marcos Hikari [UNESP]-
Autor(es): dc.creatorJorge, Roberta Jeane Bezerra-
Autor(es): dc.creatorPereira, Gustavo José Silva-
Autor(es): dc.creatorMonteiro, Helena Serra Azul-
Data de aceite: dc.date.accessioned2022-02-22T00:52:50Z-
Data de disponibilização: dc.date.available2022-02-22T00:52:50Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-01-29-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.toxicon.2020.12.004-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/208247-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/208247-
Descrição: dc.descriptionThe envenomation caused by the Bothrops pauloensis snake leads to severe local and systemic effects including acute kidney injury. In this study, we investigated the renal effects by phospholipases A2 (PLA2s), divided into two main subgroups, Asp-49 and Lys-49, isolated from the Bothrops pauloensis snake venom (BpV) in isolated rat kidney system. Both PLA2s (3 μg/mL), added alone to the perfusion system and analyzed for 120 min, had significant effects on isolated rat kidney. Asp-49 reduced Glomerular Filtration Rate (GFR) at 60, 90 and 120 min, and the percentage of total tubular sodium transport (%TNa+) and potassium transport (%TK+) at 120 min. Lys-49 increased Perfusion Pressure (PP) at 120 min and reduced GFR, %TNa+ and the percentage of total tubular chloride transport (%TCl−) at 60, 90 and 120 min. Cytokine release in the kidney tissues were increased with Asp-49 PLA2 (IL-10) and Lys-49 PLA2 (TNF-α, IL-1β, IL-10). Both increased MPO activity. Asp-49 PLA2 decreased Glutathione (GSH) and increased nitrite levels, while Lys-49 PLA2 increased Malondialdehyde (MDA), GSH and nitrite levels. Histological analysis of the perfused kidneys revealed the presence of glomerular degeneration and atrophy, deposit of proteinaceous material in Bowman's space and intratubular with both PLA2s. These findings indicated that both PLA2s modified the functional parameters in an isolated perfused kidney model with increased oxidative stress and cytokine release. PLA2s are one of the components at high concentration in BpV and our results provide important knowledge about their involvement with the nephrotoxic mechanism.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionUniversity of Cambridge-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionDepartment of Physiology and Pharmacology School of Medicine Federal University of Ceara, Coronel Nunes de Melo St., 60.430-275-
Descrição: dc.descriptionDrug Research and Development Center (NPDM) Federal University of Ceara, Coronel Nunes de Melo St., 60.430-275-
Descrição: dc.descriptionDepartment of Pharmacology Escola Paulista de Medicina Universidade Federal de São Paulo, Três de Maio St., 04.044-020-
Descrição: dc.descriptionPostgraduate Program in Veterinary Science School of Veterinary State University of Ceara-
Descrição: dc.descriptionDepartment of Biological and Environmental Sciences São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Biological and Environmental Sciences São Paulo State University (UNESP)-
Descrição: dc.descriptionFAPESP: 2017/10863-7 (GJSP)-
Formato: dc.format31-38-
Idioma: dc.languageen-
Relação: dc.relationToxicon-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAcute kidney injury-
Palavras-chave: dc.subjectCytokines-
Palavras-chave: dc.subjectKidney perfusion-
Palavras-chave: dc.subjectOxidative stress-
Palavras-chave: dc.subjectToxins-
Palavras-chave: dc.subjectTubular damage-
Título: dc.titleBothrops pauloensis snake venom-derived Asp-49 and Lys-49 phospholipases A2 mediates acute kidney injury by oxidative stress and release of inflammatory cytokines-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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