Turbidimetric method: A new, ecological, and fast way to evaluate of vancomycin potency

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUniversidade Federal de Goiás (UFG)-
Autor(es): dc.creatordo Nascimento, Patrícia Aleixa [UNESP]-
Autor(es): dc.creatorKogawa, Ana Carolina [UNESP]-
Autor(es): dc.creatorNunes Salgado, Hérida Regina [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:52:42Z-
Data de disponibilização: dc.date.available2022-02-22T00:52:42Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2019-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1093/JAOACINT/QSAA068-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/208201-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/208201-
Descrição: dc.descriptionBackground: Vancomycin, an antimicrobial, has many microbiological methods in literature, but it was not found any that follows the green chemistry principles. Objective: The aim of this work was to develop and validate a new microbiological analytical method with a green view to determine the vancomycin potency in lyophilized powder using less quantity of diluents and culture medium, minimizing the costs and reducing the time of analysis. Methods: The objective will be achieved using the microbiological method by turbidimetry. Results: Water was used as the diluent to prepare the vancomycin solution. BHI broth as used as culture media for the growth of the S. aureus ATCC 25923. The method was linear in the range of 30, 39 and 50.7 mg/mL. It was selective, with vancomycin reference and sample absorbance values very similar. The precision of the method was proved at intraday (RSD 4.42 %), interday (RSD 3.56 %) and intermediate levels (RSD 2.03%). It was accurate with mean recovery of 100.71 % and robust when changes were performed in three parameters of the method and analyzed by the F-Test and t-Test. Conclusions: The method for evaluating the potency of vancomycin in pharmaceutical product was successfully developed and validated. Highlights: The method can be applied to routine quality control of vancomycin product as an alternative that contemplates the green analytical chemistry and the current pharmaceutical analyzes.-
Descrição: dc.descriptionDepartment of Pharmaceutics School of Pharmaceutical Sciences of Araraquara Univ Estadual Paulista-UNESP-
Descrição: dc.descriptionLaboratório de Controle de Qualidade Faculdade de Farmácia Universidade Federal de Goiás-UFG-
Descrição: dc.descriptionDepartment of Pharmaceutics School of Pharmaceutical Sciences of Araraquara Univ Estadual Paulista-UNESP-
Formato: dc.format1582-1587-
Idioma: dc.languageen-
Relação: dc.relationJournal of AOAC International-
???dc.source???: dc.sourceScopus-
Título: dc.titleTurbidimetric method: A new, ecological, and fast way to evaluate of vancomycin potency-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

Não existem arquivos associados a este item.