A green, simplified, and efficient experimental setup for a high-throughput screening of agri-food by-products – From polar to nonpolar metabolites in sugarcane solid residues

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorFuture Industries Institute-
Autor(es): dc.creatorAssirati, Júlia [UNESP]-
Autor(es): dc.creatorRinaldo, Daniel [UNESP]-
Autor(es): dc.creatorRabelo, Sarita Cândida [UNESP]-
Autor(es): dc.creatorBolzani, Vanderlan da Silva [UNESP]-
Autor(es): dc.creatorHilder, Emily Frances-
Autor(es): dc.creatorFunari, Cristiano Soleo [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:52:39Z-
Data de disponibilização: dc.date.available2022-02-22T00:52:39Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2020-12-19-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.chroma.2020.461693-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/208181-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/208181-
Descrição: dc.descriptionFrom an environmental perspective, searching for useful compounds in agri-food by-products by employing inefficient and polluting analytical procedures is paradoxical. This work aimed to develop a green, simplified, and highly efficient experimental setup for extracting and tentatively identifying the broadest range of metabolites in sugarcane solid by-products collected directly within the industrial mills. Nine different extraction approaches were investigated side-by-side, including three reference methods. Based on the extraction and environmental performances assessed by two complementary metrics called Analytical-Eco Scale and the Analytical Greenness Calculator, it was possible to reach two highly efficient two liquid-phase extractions while avoiding harmful solvents and traditional time, energy, and solvent consuming sample preparation steps, such as solvent evaporation, metabolite concentration, re-suspension, and derivatization. The simultaneously produced hydroethanolic and n-heptane extracts were directly analyzed by ultra-high-performance liquid chromatography and gas chromatography, both coupled to mass spectrometry, respectively, leading to the annotation of a large dynamic range of compounds from information rich spectral data. Up to 111 metabolites were identified in a single matrix, from highly polar sucrose to nonpolar wax ester C53 in a single extraction. Orientin, apigenin-6-C-glucosylrhamnoside, 1-octacosanol, octacosanal, and other bioactive compounds were identified in these abundantly available by-products, which are currently just burned to produce energy. The best two methods developed here (Two-Liquid-Phase Ultrasound-Assisted Extraction with Probe and Two-Liquid-Phase Dynamic Maceration) appeared as a green, simplified, and highly efficient procedures to qualitatively profile metabolites in complex solid matrices.-
Descrição: dc.descriptionSão Paulo State University (UNESP) School of Agricultural Sciences, Av. Universitária 3780-
Descrição: dc.descriptionSão Paulo State University (UNESP) School of Sciences, Av. Eng. Luiz Edmundo Carrijo Coube 14-01-
Descrição: dc.descriptionSão Paulo State University (UNESP) Institute of Chemistry, R. Prof. Francisco Degni 55-
Descrição: dc.descriptionUniversity of South Australia (UniSA) Future Industries Institute, X Building-
Descrição: dc.descriptionSão Paulo State University (UNESP) School of Agricultural Sciences, Av. Universitária 3780-
Descrição: dc.descriptionSão Paulo State University (UNESP) School of Sciences, Av. Eng. Luiz Edmundo Carrijo Coube 14-01-
Descrição: dc.descriptionSão Paulo State University (UNESP) Institute of Chemistry, R. Prof. Francisco Degni 55-
Idioma: dc.languageen-
Relação: dc.relationJournal of Chromatography A-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAgricultural waste-
Palavras-chave: dc.subjectGreen analytical chemistry-
Palavras-chave: dc.subjectMetabolite profiling-
Palavras-chave: dc.subjectSample preparation-
Palavras-chave: dc.subjectTwo-liquid-phase extraction-
Título: dc.titleA green, simplified, and efficient experimental setup for a high-throughput screening of agri-food by-products – From polar to nonpolar metabolites in sugarcane solid residues-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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