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Metadados | Descrição | Idioma |
---|---|---|
Autor(es): dc.contributor | Universidade Federal de Sergipe (UFS) | - |
Autor(es): dc.contributor | Projeto Irrigacao Senador Nilo Coelho S/n | - |
Autor(es): dc.contributor | S/n | - |
Autor(es): dc.contributor | Universidade Estadual Paulista (Unesp) | - |
Autor(es): dc.creator | Ely, Valessa Lunkes | - |
Autor(es): dc.creator | Costa, Mateus Matiuzzi Da | - |
Autor(es): dc.creator | Oliveira, Helinando Pequeno De | - |
Autor(es): dc.creator | Silva Junior, Fernando Antonio Gomes Da | - |
Autor(es): dc.creator | Pereira, Daniela Isabel Brayer | - |
Autor(es): dc.creator | Soares, Mauro Pereira | - |
Autor(es): dc.creator | Vargas, Agueda Castagna De | - |
Autor(es): dc.creator | Sangioni, Luis Antonio | - |
Autor(es): dc.creator | Cargnelutti, Juliana Felipetto | - |
Autor(es): dc.creator | Ribeiro, Marcio Garcia [UNESP] | - |
Autor(es): dc.creator | Botton, Sonia De Avila | - |
Data de aceite: dc.date.accessioned | 2022-02-22T00:52:32Z | - |
Data de disponibilização: dc.date.available | 2022-02-22T00:52:32Z | - |
Data de envio: dc.date.issued | 2021-06-25 | - |
Data de envio: dc.date.issued | 2021-06-25 | - |
Data de envio: dc.date.issued | 2020-10-31 | - |
Fonte completa do material: dc.identifier | http://dx.doi.org/10.1093/mmy/myaa021 | - |
Fonte completa do material: dc.identifier | http://hdl.handle.net/11449/208143 | - |
Fonte: dc.identifier.uri | http://educapes.capes.gov.br/handle/11449/208143 | - |
Descrição: dc.description | Algae of the genus Prototheca are microorganisms involved in the occurrence of diseases in humans and animals. In bovine species, Prototheca spp. cause environmental mastitis, productive losses in dairy herds, mainly leading to the discard of infected cows. Currently, there are no effective anti-Prototheca spp. drugs to combat this infection. Thus, the search for an efficacious therapy for Prototheca spp. infections have become essential. Highly soluble polypyrrole (Ppy) is a molecule with known antimicrobial activity. This study aimed to characterize Prototheca spp. isolates from bovine mastitis as well as to evaluate the susceptibility profile and to verify the morphological alterations on Prototheca spp. isolates treated with Ppy. In this research, 36 Brazilian isolates of Prototheca spp. were characterized by restriction fragment length polymorphism polymerase chain reaction (RFLP-PCR) assay for the mitochondrial cytB gene. Additionally, Ppy algicidal activity against these isolates of Prototheca spp. was assessed by minimal microbicidal concentration method in microplates. Further, scanning electron microscopy (SEM) was performed in order to verify the morphological alterations on Prototheca spp. isolates in response to Ppy. The isolates were characterized as belonging to Prototheca zopfii genotype 2 (35/36) and Prototheca blaschkeae (1/36). Ppy had an algicidal effect on all isolates tested at concentrations ranging from 15.625 μg ml-1 to 62.5 μg ml-1. SEM showed changes on planktonic and sessile P. zopfii, including a decrease of the number of cells with the presence of an amorphous substance involving the cells. The algicidal activity of Ppy suggests the therapeutic potential of this molecule in the prevention and treatment of Prototheca spp. in bovine mastitis. | - |
Descrição: dc.description | Postgraduate Program in Veterinary Medicine (PPGMV) Department of Preventive Veterinary Medicine (DMVP) Federal University of Santa Maria (UFSM)., Av. Roraima, 1000, Predio 44, Sala 5007, Bairro Camobi | - |
Descrição: dc.description | Federal University of Sao Francisco Valley Projeto Irrigacao Senador Nilo Coelho S/n, Rodovia BR 407 - Km 12, Lote 543 | - |
Descrição: dc.description | Departament of Microbiology and Parasitology Instituto de Biologia Federal University of Pelotas Campus Universitario Capao Do Leao S/n, Predio 18, Sala 14. | - |
Descrição: dc.description | Faculty of Veterinary Medicine Federal University of Pelotas Campus Universitario Capao Do Leao S/n | - |
Descrição: dc.description | Department of Veterinary Hygiene and Public Health Sao Paulo State University - UNESP | - |
Descrição: dc.description | Department of Veterinary Hygiene and Public Health Sao Paulo State University - UNESP | - |
Formato: dc.format | 1114-1119 | - |
Idioma: dc.language | en | - |
Relação: dc.relation | Medical Mycology | - |
???dc.source???: dc.source | Scopus | - |
Palavras-chave: dc.subject | algae | - |
Palavras-chave: dc.subject | algicidal effect | - |
Palavras-chave: dc.subject | mastitis | - |
Palavras-chave: dc.subject | polypyrrole | - |
Palavras-chave: dc.subject | Prototheca spp | - |
Título: dc.title | In vitro algicidal effect of polypyrrole on Prototheca species isolates from bovine mastitisAlgicidal activity of polypyrrole on Prototheca spp. | - |
Tipo de arquivo: dc.type | livro digital | - |
Aparece nas coleções: | Repositório Institucional - Unesp |
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