In Vitro Anti-Inflammatory Activity in Arthritic Synoviocytes of A. brachypoda Root Extracts and Its Unusual Dimeric Flavonoids

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Autor(es): dc.contributorUniversity of Geneva-
Autor(es): dc.contributorUniversidade Federal do Ceará-
Autor(es): dc.contributorCentro de Ciência Exatas e Tecnologia-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.creatorSalgado, Carlota-
Autor(es): dc.creatorMorin, Hugo-
Autor(es): dc.creatorCoriolano de Aquino, Nayara-
Autor(es): dc.creatorNeff, Laurence-
Autor(es): dc.creatorQuintino da Rocha, Cláudia-
Autor(es): dc.creatorVilegas, Wagner [UNESP]-
Autor(es): dc.creatorMarcourt, Laurence-
Autor(es): dc.creatorWolfender, Jean-Luc-
Autor(es): dc.creatorJordan, Olivier-
Autor(es): dc.creatorFerreira Queiroz, Emerson-
Autor(es): dc.creatorAllémann, Eric-
Data de aceite: dc.date.accessioned2022-02-22T00:52:32Z-
Data de disponibilização: dc.date.available2022-02-22T00:52:32Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2020-11-08-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/molecules25215219-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/208142-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/208142-
Descrição: dc.descriptionArrabidaea brachypoda is a plant commonly used for the treatment of kidney stones, arthritis and pain in traditional Brazilian medicine. Different in vitro and in vivo activities, ranging from antinociceptive to anti-Trypanosoma cruzi, have been reported for the dichloromethane root extract of Arrabidaea brachypoda (DCMAB) and isolated compounds. This work aimed to assess the in vitro anti-inflammatory activity in arthritic synoviocytes of the DCMAB, the hydroethanolic extract (HEAB) and three dimeric flavonoids isolated from the DCMAB. These compounds, brachydin A (1), B (2) and C (3), were isolated both by medium pressure liquid and high-speed counter current chromatography. Their quantification was performed by mass spectrometry on both DCMAB and HEAB. IL-1β activated human fibroblast-like synoviocytes were incubated with both extracts and isolated compounds to determine the levels of pro-inflammatory cytokine IL-6 by enzyme-linked immunosorbent assay (ELISA). DCMAB inhibited 30% of IL-6 release at 25 µg/mL, when compared with controls while HEAB was inactive. IC50 values determined for 2 and 3 were 3-fold higher than 1. The DCMAB activity seems to be linked to higher proportions of compounds 2 and 3 in this extract. These observations could thus explain the traditional use of A. brachypoda roots in the treatment of osteoarthritis.-
Descrição: dc.descriptionSchool of Pharmaceutical Sciences University of Geneva-
Descrição: dc.descriptionUniversity of Geneva, Institute of Pharmaceutical Sciences of Western Switzerland-
Descrição: dc.descriptionDepartamento de Química Orgânica e Inorgânica Universidade Federal do Ceará-
Descrição: dc.descriptionLaboratório de Produtos Naturais Centro de Ciência Exatas e Tecnologia Departamento de Química-
Descrição: dc.descriptionExperimental Campus of the Paulista Coast UNESP-São Paulo State University-
Descrição: dc.descriptionExperimental Campus of the Paulista Coast UNESP-São Paulo State University-
Idioma: dc.languageen-
Relação: dc.relationMolecules (Basel, Switzerland)-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectanti-inflammatory activity-
Palavras-chave: dc.subjectArrabidaea brachypoda-
Palavras-chave: dc.subjectflavonoids-
Palavras-chave: dc.subjecthigh-speed counter current chromatography-
Palavras-chave: dc.subjectmass-spectrometric quantification-
Palavras-chave: dc.subjectosteoarthritis-
Título: dc.titleIn Vitro Anti-Inflammatory Activity in Arthritic Synoviocytes of A. brachypoda Root Extracts and Its Unusual Dimeric Flavonoids-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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