Label-free capacitive assaying of biomarkers for molecular diagnostics

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.creatorGarrote, Beatriz L. [UNESP]-
Autor(es): dc.creatorSantos, Adriano [UNESP]-
Autor(es): dc.creatorBueno, Paulo R. [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:52:19Z-
Data de disponibilização: dc.date.available2022-02-22T00:52:19Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2020-11-30-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1038/s41596-020-0390-9-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/208071-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/208071-
Descrição: dc.descriptionThe label-free analysis of biomarkers offers important advantages in developing point-of-care (PoC) biosensors. In contrast to label-based methodologies, such as ELISA, label-free analysis enables direct detection of targets without additional steps and labeled reagents. Nonetheless, label-free approaches require high sensitivity to detect the intrinsic features of a biomarker and low levels of nonspecific signals. Electrochemical capacitance, Cμ¯, is a feature of electroactive nanoscale films that can be measured using electrochemical impedance spectroscopy. Cμ¯ is promising as an electrochemical transducing signal for the development of high-sensitivity, reagentless and label-free molecular diagnostic assays. We used a proprietary ferrocene (Fc)-tagged peptide that is able to self-assemble onto gold electrodes (thicknesses <2 nm) to which any biological receptor can be coupled. When coupled with biological receptors (e.g., a monoclonal antibody), Cμ¯ exhibited by the redox-tagged peptide changes as a function of the target concentration. We provide herein the steps for the qualitative and quantitative detection of dengue non-structural protein 1 (NS1) biomarker. Detection of NS1 can be used to diagnose dengue virus infection, which causes epidemics each year in tropical and subtropical regions of the world. Including the pre-treatment of the electrode surface, the analysis takes ~25 h. This time can be reduced to minutes if the electrode surface is fabricated separately, demonstrating that Cμ¯ is promising for PoC applications. We hope this protocol will serve as a reference point for researchers and companies that intend to further develop capacitive devices for molecular diagnostic assays.-
Descrição: dc.descriptionInstitute of Chemistry São Paulo State University (UNESP Universidade Estadual Paulista)-
Descrição: dc.descriptionInstitute of Chemistry São Paulo State University (UNESP Universidade Estadual Paulista)-
Formato: dc.format3879-3893-
Idioma: dc.languageen-
Relação: dc.relationNature Protocols-
???dc.source???: dc.sourceScopus-
Título: dc.titleLabel-free capacitive assaying of biomarkers for molecular diagnostics-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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