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Metadados | Descrição | Idioma |
---|---|---|
Autor(es): dc.contributor | Universidade Estadual Paulista (Unesp) | - |
Autor(es): dc.creator | Garrote, Beatriz L. [UNESP] | - |
Autor(es): dc.creator | Santos, Adriano [UNESP] | - |
Autor(es): dc.creator | Bueno, Paulo R. [UNESP] | - |
Data de aceite: dc.date.accessioned | 2022-02-22T00:52:19Z | - |
Data de disponibilização: dc.date.available | 2022-02-22T00:52:19Z | - |
Data de envio: dc.date.issued | 2021-06-25 | - |
Data de envio: dc.date.issued | 2021-06-25 | - |
Data de envio: dc.date.issued | 2020-11-30 | - |
Fonte completa do material: dc.identifier | http://dx.doi.org/10.1038/s41596-020-0390-9 | - |
Fonte completa do material: dc.identifier | http://hdl.handle.net/11449/208071 | - |
Fonte: dc.identifier.uri | http://educapes.capes.gov.br/handle/11449/208071 | - |
Descrição: dc.description | The label-free analysis of biomarkers offers important advantages in developing point-of-care (PoC) biosensors. In contrast to label-based methodologies, such as ELISA, label-free analysis enables direct detection of targets without additional steps and labeled reagents. Nonetheless, label-free approaches require high sensitivity to detect the intrinsic features of a biomarker and low levels of nonspecific signals. Electrochemical capacitance, Cμ¯, is a feature of electroactive nanoscale films that can be measured using electrochemical impedance spectroscopy. Cμ¯ is promising as an electrochemical transducing signal for the development of high-sensitivity, reagentless and label-free molecular diagnostic assays. We used a proprietary ferrocene (Fc)-tagged peptide that is able to self-assemble onto gold electrodes (thicknesses <2 nm) to which any biological receptor can be coupled. When coupled with biological receptors (e.g., a monoclonal antibody), Cμ¯ exhibited by the redox-tagged peptide changes as a function of the target concentration. We provide herein the steps for the qualitative and quantitative detection of dengue non-structural protein 1 (NS1) biomarker. Detection of NS1 can be used to diagnose dengue virus infection, which causes epidemics each year in tropical and subtropical regions of the world. Including the pre-treatment of the electrode surface, the analysis takes ~25 h. This time can be reduced to minutes if the electrode surface is fabricated separately, demonstrating that Cμ¯ is promising for PoC applications. We hope this protocol will serve as a reference point for researchers and companies that intend to further develop capacitive devices for molecular diagnostic assays. | - |
Descrição: dc.description | Institute of Chemistry São Paulo State University (UNESP Universidade Estadual Paulista) | - |
Descrição: dc.description | Institute of Chemistry São Paulo State University (UNESP Universidade Estadual Paulista) | - |
Formato: dc.format | 3879-3893 | - |
Idioma: dc.language | en | - |
Relação: dc.relation | Nature Protocols | - |
???dc.source???: dc.source | Scopus | - |
Título: dc.title | Label-free capacitive assaying of biomarkers for molecular diagnostics | - |
Tipo de arquivo: dc.type | livro digital | - |
Aparece nas coleções: | Repositório Institucional - Unesp |
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