Steinernema diaprepesi (Rhabditida: Steinernematidae) parasitizing Gonipterus platensis (Coleoptera: Curculionidae): EPN can control Gonipterus platensis

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUniversidade Federal de Viçosa (UFV)-
Autor(es): dc.creatorDamascena, Alixelhe Pacheco [UNESP]-
Autor(es): dc.creatorDe Carvalho, Vanessa Rafaela [UNESP]-
Autor(es): dc.creatorRibeiro, Murilo Fonseca [UNESP]-
Autor(es): dc.creatorHorta, André Ballerini [UNESP]-
Autor(es): dc.creatorMonteiro De Castro E Castro, Bárbara-
Autor(es): dc.creatorZanuncio, Antônio José Vinha-
Autor(es): dc.creatorWilcken, Carlos Frederico [UNESP]-
Autor(es): dc.creatorZanuncio, José Cola-
Autor(es): dc.creatorWilcken, Silvia Renata Siciliano [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:52:15Z-
Data de disponibilização: dc.date.available2022-02-22T00:52:15Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2020-08-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1098/rsos.200282rsos200282-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/208047-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/208047-
Descrição: dc.descriptionEntomopathogenic nematodes (EPNs) can control pests due to mutualistic association with bacteria that reproduce and kill the host from septicemia, making the environment favourable for nematode development and reproduction. The objective of this study was to identify an EPN isolate collected in eucalyptus cultivation and to determine its pathogenicity with regard to Gonipterus platensis Marelli (Coleoptera: Curculionidae). Four steel-mesh traps with two seventh-instar Galleria mellonella larvae were buried 5 cm deep in the soil in a commercial Eucalyptus plantation. After 7 days, the traps were packed in plastic bags and transported to laboratory to isolate the EPNs using White traps. The obtained nematodes were multiplied in G. mellonella larvae and identified by sequencing their D2/D3 expansion of the 28S rDNA region by polymerase chain reaction (PCR) and specific primers for ITS regions. Steinernema diaprepesi was identified and inoculated into G. platensis pupae at doses of 500, 1000 and 5000 infective juveniles (IJs) to determine its pathogenicity to this pest. At 8 days after inoculation, the mortality rate of the G. platensis pupae was 80% with the lowest concentration and 100% with the others. The emergence of nematodes and the rapid degradation of G. platensis pupae were observed in those inoculated with IJs. The pathogenicity to the G. platensis pupae indicates potential for using this nematode in the integrated management of this insect.-
Descrição: dc.descriptionUniversidade Estadual Paulista (UNESP) Departamento de Protecąõ Vegetal Departamento de Protecąõ Vegetal-
Descrição: dc.descriptionDepartamento de Entomologia/BIOAGRO Universidade Federal de Viçosa-
Descrição: dc.descriptionDepartamento de Engenharia Florestal Universidade Federal de Viçosa-
Descrição: dc.descriptionUniversidade Estadual Paulista (UNESP) Departamento de Protecąõ Vegetal Departamento de Protecąõ Vegetal-
Idioma: dc.languageen-
Relação: dc.relationRoyal Society Open Science-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectbiological control-
Palavras-chave: dc.subjectentomopathogenic nematodes-
Palavras-chave: dc.subjecteucalyptus weevil-
Palavras-chave: dc.subjectpathogenicity-
Título: dc.titleSteinernema diaprepesi (Rhabditida: Steinernematidae) parasitizing Gonipterus platensis (Coleoptera: Curculionidae): EPN can control Gonipterus platensis-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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