Magnetic graphene oxide as a platform for the immobilization of cellulases and xylanases: Ultrastructural characterization and assessment of lignocellulosic biomass hydrolysis

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorInstitute of Chemical Technology (ITQ)-
Autor(es): dc.contributorCzech Academy of Sciences-
Autor(es): dc.contributorMackenzie Presbyterian University-
Autor(es): dc.creatorPaz-Cedeno, Fernando Roberto [UNESP]-
Autor(es): dc.creatorCarceller, Jose Miguel-
Autor(es): dc.creatorIborra, Sara-
Autor(es): dc.creatorDonato, Ricardo Keitel-
Autor(es): dc.creatorGodoy, Anna Paula-
Autor(es): dc.creatorVeloso de Paula, Ariela [UNESP]-
Autor(es): dc.creatorMonti, Rubens [UNESP]-
Autor(es): dc.creatorCorma, Avelino-
Autor(es): dc.creatorMasarin, Fernando [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:52:08Z-
Data de disponibilização: dc.date.available2022-02-22T00:52:08Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-01-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.renene.2020.09.059-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/208000-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/208000-
Descrição: dc.descriptionFor producing second-generation ethanol (cellulosic ethanol) and other value-added bioproducts, magnetic graphene oxide (GO-MNP) was synthesized in this work and used as the immobilization support for an industrial cellulase-and xylanase-containing preparation. GO-MNP characterization by TEM, SEM and ATR-FTIR spectroscopy showed that the magnetic nanoparticles are homogeneously distributed onto the GO sheets surface. The enzymatic preparation was immobilized by means of carbodiimide cross-linking chemistry using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide (NHS). The supported final biocatalyst (GO-MNP-Enz) showed high activity for the hydrolysis of pretreated sugarcane bagasse (PSB) and presented relative endoglucanase, xylanase, β-glucosidase, and β-xylosidase activities of 70%, 66%, 88%, and 70%, respectively, after 10 cycles of hydrolysis of their respective substrates. The biocatalyst also maintained approximately 50% and 80% of its efficiency for cellulose and xylan hydrolysis, respectively, being the TOF (g.g−1.h−1) the highest observed when compared with previous results reported in literature. These findings suggest that GO-MNP-Enz may be a prospective candidate for industrial applications such as second-generation ethanol production.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionUniversitat Politècnica de València-
Descrição: dc.descriptionCentre for Lipid Research, Indian Institute of Chemical Technology-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionMinisterio de Ciencia, Innovación y Universidades-
Descrição: dc.descriptionSão Paulo State University (UNESP) School of Pharmaceutical Science (FCF) Department of Bioprocess Engineering and Biotechnology. Araraquara-SP-
Descrição: dc.descriptionUniversitat Politècnica de València (UPV) Institute of Chemical Technology (ITQ)-
Descrição: dc.descriptionInstitute of Macromolecular Chemistry Czech Academy of Sciences-
Descrição: dc.descriptionGraphene and Nanomaterials Research Center Mackenzie Presbyterian University, São Paulo-
Descrição: dc.descriptionSão Paulo State University (UNESP) School of Pharmaceutical Science (FCF) Department of Bioprocess Engineering and Biotechnology. Araraquara-SP-
Descrição: dc.descriptionFAPESP: 2018/06241–3-
Descrição: dc.descriptionMinisterio de Ciencia, Innovación y Universidades: PGC2018-097277-B-100-
Formato: dc.format491-501-
Idioma: dc.languageen-
Relação: dc.relationRenewable Energy-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectBiocatalyst-
Palavras-chave: dc.subjectEnzyme immobilization-
Palavras-chave: dc.subjectGraphene oxide-
Palavras-chave: dc.subjectMagnetic nanoparticles-
Palavras-chave: dc.subjectMonomeric fermentable sugars-
Palavras-chave: dc.subjectSugarcane bagasse hydrolysis-
Título: dc.titleMagnetic graphene oxide as a platform for the immobilization of cellulases and xylanases: Ultrastructural characterization and assessment of lignocellulosic biomass hydrolysis-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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