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Metadados | Descrição | Idioma |
---|---|---|
Autor(es): dc.contributor | Universidade Estadual Paulista (Unesp) | - |
Autor(es): dc.contributor | Brown University | - |
Autor(es): dc.contributor | Alpert Medical School & Brown University | - |
Autor(es): dc.creator | de Alvarenga, Janaína Araújo [UNESP] | - |
Autor(es): dc.creator | de Barros, Patrícia Pimentel [UNESP] | - |
Autor(es): dc.creator | de Camargo Ribeiro, Felipe [UNESP] | - |
Autor(es): dc.creator | Rossoni, Rodnei Dennis [UNESP] | - |
Autor(es): dc.creator | Garcia, Maíra Terra [UNESP] | - |
Autor(es): dc.creator | dos Santos Velloso, Marisol [UNESP] | - |
Autor(es): dc.creator | Shukla, Shashank | - |
Autor(es): dc.creator | Fuchs, Beth Burgwyn | - |
Autor(es): dc.creator | Shukla, Anita | - |
Autor(es): dc.creator | Mylonakis, Eleftherios | - |
Autor(es): dc.creator | Junqueira, Juliana Campos [UNESP] | - |
Data de aceite: dc.date.accessioned | 2022-02-22T00:52:06Z | - |
Data de disponibilização: dc.date.available | 2022-02-22T00:52:06Z | - |
Data de envio: dc.date.issued | 2021-06-25 | - |
Data de envio: dc.date.issued | 2021-06-25 | - |
Data de envio: dc.date.issued | 2021-04-01 | - |
Fonte completa do material: dc.identifier | http://dx.doi.org/10.1007/s12602-020-09712-0 | - |
Fonte completa do material: dc.identifier | http://hdl.handle.net/11449/207991 | - |
Fonte: dc.identifier.uri | http://educapes.capes.gov.br/handle/11449/207991 | - |
Descrição: dc.description | Streptococcus mutans is considered to be a major bacterium involved in dental caries, and the control of virulence mechanisms is fundamental to prevent disease. Probiotics present a promising preventive method; however, the use of probiotics requires its incorporation into delivery materials to facilitate oral colonization. Thus, we performed a comprehensive study examining preventive effects of Lactobacillus paracasei 28.4–enriched gellan hydrogel materials to inhibit S. mutans in planktonic and biofilm states, addressing its influence in the production of extracellular polysaccharides (EPS) and altered gene expression of several cariogenic virulence factors. L. paracasei 28.4, a strain isolated from the oral cavity of a caries-free individual, was incorporated in three gellan hydrogels (0.5%, 0.75%, and 1% w/v). The pretreatment with probiotic-gellan formulations provided a release of L. paracasei cells over 24 h that was sufficient to inhibit the planktonic growth of S. mutans, independent of the gellan concentrations and pH variations. This pretreatment also had inhibitory activity against S. mutans biofilms, exhibiting a reduction of 0.57 to 1.54 log10 in CFU/mL (p < 0.0001) and a decrease of 68.8 to 71.3% in total biomass (p < 0.0001) compared with the control group. These inhibitory effects were associated with the decreased production of EPS by 80% (p < 0.0001) and the downregulation of luxS, brpA, gbpB, and gtfB genes. The gellan formulation containing L. paracasei 28.4 exhibited probiotic effects for preventing S. mutans growth, biofilm formation, and production of cariogenic factors to suggest possible use in tooth decay prevention. | - |
Descrição: dc.description | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) | - |
Descrição: dc.description | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) | - |
Descrição: dc.description | Department of Biosciences and Oral Diagnosis Institute of Science and Technology São Paulo State University/UNESP, Av. Francisco José Longo 777, São Dimas | - |
Descrição: dc.description | School of Engineering Brown University | - |
Descrição: dc.description | Rhode Island Hospital Alpert Medical School & Brown University | - |
Descrição: dc.description | Department of Biosciences and Oral Diagnosis Institute of Science and Technology São Paulo State University/UNESP, Av. Francisco José Longo 777, São Dimas | - |
Descrição: dc.description | FAPESP: 2017/15529-8 | - |
Descrição: dc.description | CNPq: 306330/2018-0 | - |
Formato: dc.format | 506-517 | - |
Idioma: dc.language | en | - |
Relação: dc.relation | Probiotics and Antimicrobial Proteins | - |
???dc.source???: dc.source | Scopus | - |
Palavras-chave: dc.subject | Biofilm | - |
Palavras-chave: dc.subject | Extracellular polysaccharides | - |
Palavras-chave: dc.subject | Gellan hydrogels | - |
Palavras-chave: dc.subject | Gene expression | - |
Palavras-chave: dc.subject | Lactobacillus paracasei | - |
Palavras-chave: dc.subject | Probiotic | - |
Palavras-chave: dc.subject | Streptococcus mutans | - |
Título: dc.title | Probiotic Effects of Lactobacillus paracasei 28.4 to Inhibit Streptococcus mutans in a Gellan-Based Formulation | - |
Tipo de arquivo: dc.type | livro digital | - |
Aparece nas coleções: | Repositório Institucional - Unesp |
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