Antimicrobial and antibiofilm activity of Lys-[Trp6]hy-a1 combined with ciprofloxacin against gram-negative bacteria

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Autor(es): dc.contributorFederal University of Ceara – UFC-
Autor(es): dc.contributorUniversity Center IN-TA – UNINTA-
Autor(es): dc.contributorFederal University of Jatai-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.creatorCarneiro, Victor Alves-
Autor(es): dc.creatorde Oliveira, Simone Torres-
Autor(es): dc.creatorSilva, Rondinely Lima-
Autor(es): dc.creatorDuarte, Humberlania de Sousa-
Autor(es): dc.creatorSilva, Maria Laína-
Autor(es): dc.creatorMatos, Maria Nágila Carneiro-
Autor(es): dc.creatorCavalcante, Rafaela Mesquita Bastos-
Autor(es): dc.creatorFigueira, Ciro Siqueira-
Autor(es): dc.creatorLorenzón, Esteban Nicolás-
Autor(es): dc.creatorCilli, Eduardo Maffud [UNESP]-
Autor(es): dc.creatorda Cunha, Rodrigo Maranguape Silva-
Data de aceite: dc.date.accessioned2022-02-22T00:52:00Z-
Data de disponibilização: dc.date.available2022-02-22T00:52:00Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2019-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.2174/0929866527666200416145549-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/207966-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/207966-
Descrição: dc.descriptionBackground: Ciprofloxacin (Cip) is the most commonly used quinolone in clinical prac-tice; however large-scale use has favored the increase of multiresistant pathogenic microorganisms. Antimicrobial peptides (AMPs) appear to be a promising alternative in potentiating these conven-tional drugs. Objective: The aim of this study was to evaluate the effect of the peptide Lys-[Trp6]hy-a1 (lys-a1) on the antimicrobial and antibiofilm activity of ciprofloxacin against clinically relevant gram-nega-tive bacteria. Methods: The antimicrobial effects of Cip and lys-a1 were assessed by determining the minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs). The synergistic action of Cip and lys-a1 was determined by checkerboard assay. The time-kill curve was con-structed for the Cip/lys-a1 combination against Pseudomonas aeruginosa ATCC 9027. The antibio-film activity of this combination was analyzed by crystal violet, colony-forming unit count and atomic force microscopy (AFM). Results: The data demonstrated that lys-a1 was able to inhibit planktonic growth of strains of P. aeruginosa and Klebsiella pneumoniae both at 125 μg/mL. The fractional inhibitory concentration index (FICi) showed a synergistic effect between Cip and lys-a1 against P. aeruginosa, decreasing the MICs of the individual antimicrobial agents by 4-and 8-fold, respectively. This effect was also observed for the death kinetics and antibiofilm activity. Analysis of the early biofilms (6 h) as well as isolated cells by AFM images evidenced the cell perturbation caused by Cip/lys-a1 treatment. Conclusion: These data suggest that lys-a1 has biotechnological potential as a therapeutic tool for the treatment of infections caused by clinically relevant microorganisms, especially P. aeruginosa.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionRijksuniversiteit Groningen-
Descrição: dc.descriptionLaboratory of Biofilms and Antimicrobial Agents (LaBAM) Faculty of Medicine Federal University of Ceara – UFC-
Descrição: dc.descriptionCenter for Bioprospecting and Applied Molecular Experimentation (NUBEM) University Center IN-TA – UNINTA-
Descrição: dc.descriptionMedical Research Laboratory Federal University of Jatai-
Descrição: dc.descriptionDepartment of Biochemistry and Chemical Technology Estadual University of Sao Paulo – UNESP-
Descrição: dc.descriptionDepartment of Biochemistry and Chemical Technology Estadual University of Sao Paulo – UNESP-
Formato: dc.format1124-1131-
Idioma: dc.languageen-
Relação: dc.relationProtein and Peptide Letters-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAntibiofilm activity-
Palavras-chave: dc.subjectAntimicrobial peptide-
Palavras-chave: dc.subjectCiprofloxacin-
Palavras-chave: dc.subjectLys-a1-
Palavras-chave: dc.subjectPseudomonas aeruginosa-
Palavras-chave: dc.subjectSynergism-
Título: dc.titleAntimicrobial and antibiofilm activity of Lys-[Trp6]hy-a1 combined with ciprofloxacin against gram-negative bacteria-
Tipo de arquivo: dc.typelivro digital-
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