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Metadados | Descrição | Idioma |
---|---|---|
Autor(es): dc.contributor | Universidade de São Paulo (USP) | - |
Autor(es): dc.contributor | School of Animal Sciences and Food Engineering | - |
Autor(es): dc.contributor | Universidade Estadual Paulista (Unesp) | - |
Autor(es): dc.creator | Pieri, Naira Caroline Godoy | - |
Autor(es): dc.creator | de Souza, Aline Fernanda | - |
Autor(es): dc.creator | Botigelli, Ramon Cesar [UNESP] | - |
Autor(es): dc.creator | Pessôa, Laís Vicari de Figueiredo | - |
Autor(es): dc.creator | Recchia, Kaiana | - |
Autor(es): dc.creator | Machado, Lucas Simões | - |
Autor(es): dc.creator | Glória, Mayra Hirakawa | - |
Autor(es): dc.creator | de Castro, Raquel Vasconcelos Guimarães [UNESP] | - |
Autor(es): dc.creator | Leal, Diego Feitosa | - |
Autor(es): dc.creator | Fantinato Neto, Paulo | - |
Autor(es): dc.creator | Martins, Simone Maria Massami Kitamura | - |
Autor(es): dc.creator | dos Santos Martins, Daniele | - |
Autor(es): dc.creator | Bressan, Fabiana Fernandes | - |
Autor(es): dc.creator | de Andrade, André Furugen Cesar | - |
Data de aceite: dc.date.accessioned | 2022-02-22T00:51:58Z | - |
Data de disponibilização: dc.date.available | 2022-02-22T00:51:58Z | - |
Data de envio: dc.date.issued | 2021-06-25 | - |
Data de envio: dc.date.issued | 2021-06-25 | - |
Data de envio: dc.date.issued | 2020-12-31 | - |
Fonte completa do material: dc.identifier | http://dx.doi.org/10.1007/s12015-021-10198-8 | - |
Fonte completa do material: dc.identifier | http://hdl.handle.net/11449/207956 | - |
Fonte: dc.identifier.uri | http://educapes.capes.gov.br/handle/11449/207956 | - |
Descrição: dc.description | Culture conditions regulate the process of pluripotency acquisition and self-renewal. This study aimed to analyse the influence of the in vitro environment on the induction of porcine induced pluripotent stem cell (piPSCs) differentiation into primordial germ cell-like cells (pPGCLCs). piPSC culture with different supplementation strategies (LIF, bFGF, or LIF plus bFGF) promoted heterogeneous phenotypic profiles. Continuous bFGF supplementation during piPSCs culture was beneficial to support a pluripotent state and the differentiation of piPSCs into pPGCLCs. The pPGCLCs were positive for the gene and protein expression of pluripotent and germinative markers. This study can provide a suitable in vitro model for use in translational studies and to help answer numerous remaining questions about germ cells. Graphical abstract: [Figure not available: see fulltext.] | - |
Descrição: dc.description | Department of Animal Reproduction School of Veterinary Medicine and Animal Science University of São Paulo | - |
Descrição: dc.description | Department of Veterinary Medicine School of Animal Sciences and Food Engineering | - |
Descrição: dc.description | Department of Pharmacology Institute of Biosciences São Paulo State University (UNESP) | - |
Descrição: dc.description | Department of Surgery Faculty of Veterinary Medicine and Animal Sciences University of Sao Paulo | - |
Descrição: dc.description | Department of Biochemistry Paulista School of Medicine Federal University of São Paulo (UNIFESP) | - |
Descrição: dc.description | Department of Preventive Veterinary Medicine and Animal Reproduction Faculty of Agricultural and Veterinary Sciences São Paulo State University (UNESP) | - |
Descrição: dc.description | Department of Animal Science Faculty of Animal Science and Food Engineering University of São Paulo | - |
Descrição: dc.description | Department of Pharmacology Institute of Biosciences São Paulo State University (UNESP) | - |
Descrição: dc.description | Department of Preventive Veterinary Medicine and Animal Reproduction Faculty of Agricultural and Veterinary Sciences São Paulo State University (UNESP) | - |
Idioma: dc.language | en | - |
Relação: dc.relation | Stem Cell Reviews and Reports | - |
???dc.source???: dc.source | Scopus | - |
Palavras-chave: dc.subject | PGCs | - |
Palavras-chave: dc.subject | Porcine | - |
Palavras-chave: dc.subject | Regenerative medicine | - |
Palavras-chave: dc.subject | Reproduction | - |
Palavras-chave: dc.subject | Translational medicine | - |
Título: dc.title | Porcine Primordial Germ Cell-Like Cells Generated from Induced Pluripotent Stem Cells Under Different Culture Conditions | - |
Tipo de arquivo: dc.type | livro digital | - |
Aparece nas coleções: | Repositório Institucional - Unesp |
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