Porcine Primordial Germ Cell-Like Cells Generated from Induced Pluripotent Stem Cells Under Different Culture Conditions

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Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorSchool of Animal Sciences and Food Engineering-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.creatorPieri, Naira Caroline Godoy-
Autor(es): dc.creatorde Souza, Aline Fernanda-
Autor(es): dc.creatorBotigelli, Ramon Cesar [UNESP]-
Autor(es): dc.creatorPessôa, Laís Vicari de Figueiredo-
Autor(es): dc.creatorRecchia, Kaiana-
Autor(es): dc.creatorMachado, Lucas Simões-
Autor(es): dc.creatorGlória, Mayra Hirakawa-
Autor(es): dc.creatorde Castro, Raquel Vasconcelos Guimarães [UNESP]-
Autor(es): dc.creatorLeal, Diego Feitosa-
Autor(es): dc.creatorFantinato Neto, Paulo-
Autor(es): dc.creatorMartins, Simone Maria Massami Kitamura-
Autor(es): dc.creatordos Santos Martins, Daniele-
Autor(es): dc.creatorBressan, Fabiana Fernandes-
Autor(es): dc.creatorde Andrade, André Furugen Cesar-
Data de aceite: dc.date.accessioned2022-02-22T00:51:58Z-
Data de disponibilização: dc.date.available2022-02-22T00:51:58Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2020-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1007/s12015-021-10198-8-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/207956-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/207956-
Descrição: dc.descriptionCulture conditions regulate the process of pluripotency acquisition and self-renewal. This study aimed to analyse the influence of the in vitro environment on the induction of porcine induced pluripotent stem cell (piPSCs) differentiation into primordial germ cell-like cells (pPGCLCs). piPSC culture with different supplementation strategies (LIF, bFGF, or LIF plus bFGF) promoted heterogeneous phenotypic profiles. Continuous bFGF supplementation during piPSCs culture was beneficial to support a pluripotent state and the differentiation of piPSCs into pPGCLCs. The pPGCLCs were positive for the gene and protein expression of pluripotent and germinative markers. This study can provide a suitable in vitro model for use in translational studies and to help answer numerous remaining questions about germ cells. Graphical abstract: [Figure not available: see fulltext.]-
Descrição: dc.descriptionDepartment of Animal Reproduction School of Veterinary Medicine and Animal Science University of São Paulo-
Descrição: dc.descriptionDepartment of Veterinary Medicine School of Animal Sciences and Food Engineering-
Descrição: dc.descriptionDepartment of Pharmacology Institute of Biosciences São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Surgery Faculty of Veterinary Medicine and Animal Sciences University of Sao Paulo-
Descrição: dc.descriptionDepartment of Biochemistry Paulista School of Medicine Federal University of São Paulo (UNIFESP)-
Descrição: dc.descriptionDepartment of Preventive Veterinary Medicine and Animal Reproduction Faculty of Agricultural and Veterinary Sciences São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Animal Science Faculty of Animal Science and Food Engineering University of São Paulo-
Descrição: dc.descriptionDepartment of Pharmacology Institute of Biosciences São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Preventive Veterinary Medicine and Animal Reproduction Faculty of Agricultural and Veterinary Sciences São Paulo State University (UNESP)-
Idioma: dc.languageen-
Relação: dc.relationStem Cell Reviews and Reports-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectPGCs-
Palavras-chave: dc.subjectPorcine-
Palavras-chave: dc.subjectRegenerative medicine-
Palavras-chave: dc.subjectReproduction-
Palavras-chave: dc.subjectTranslational medicine-
Título: dc.titlePorcine Primordial Germ Cell-Like Cells Generated from Induced Pluripotent Stem Cells Under Different Culture Conditions-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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