Diamond nanoparticles-porphyrin mthpp conjugate as photosensitizing platform: Cytotoxicity and antibacterial activity

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorFederal Institute of São Paulo (IFSP)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorHelmholtz Zentrum München-
Autor(es): dc.contributorTechnological Institute of Aeronautics (ITA)-
Autor(es): dc.contributorNational Institute for Space Research (INPE)-
Autor(es): dc.contributorUniversity of Johannesburg-
Autor(es): dc.creatorHurtado, Carolina Ramos-
Autor(es): dc.creatorHurtado, Gabriela Ramos [UNESP]-
Autor(es): dc.creatorde Cena, Gabrielle Lupeti-
Autor(es): dc.creatorQueiroz, Rafaela Campos-
Autor(es): dc.creatorSilva, Alexandre Vieira-
Autor(es): dc.creatorDiniz, Milton Faria-
Autor(es): dc.creatorDos Santos, Verônica Ribeiro-
Autor(es): dc.creatorTrava-Airoldi, Vladimir-
Autor(es): dc.creatorBaptista, Maurício da Silva-
Autor(es): dc.creatorTsolekile, Ncediwe-
Autor(es): dc.creatorOluwafemi, Oluwatobi Samuel-
Autor(es): dc.creatorConceição, Katia-
Autor(es): dc.creatorTada, Dayane Batista-
Data de aceite: dc.date.accessioned2022-02-22T00:51:53Z-
Data de disponibilização: dc.date.available2022-02-22T00:51:53Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/nano11061393-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/207948-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/207948-
Descrição: dc.descriptionConjugation of photosensitizers (PS) with nanoparticles has been largely used as a strategy to stabilize PS in the biological medium resulting in photosensitizing nanoparticles of enhanced photoactivity. Herein, (Meso-5, 10, 15, 20-tetrakis (3-hydroxyphenyl) phorphyryn (mTHPP) was conjugated with diamond nanoparticles (ND) by covalent bond. Nanoconjugate ND-mTHPP showed suitable stability in aqueous suspension with 58 nm of hydrodynamic diameter and Zeta potential of −23 mV. The antibacterial activity of ND-mTHPP was evaluated against Escherichia coli for different incubation times (0–24 h). The optimal activity was observed after 2 h of incubation and irradiation (660 nm; 51 J/cm2 ) performed right after the addition of ND-mTHPP (100 µg/mL) to the bacterial suspension. The inhibitory activity was 56% whereas ampicillin at the same conditions provided only 14% of bacterial growth inhibition. SEM images showed agglomerate of ND-mTHPP adsorbed on the bacterial cell wall, suggesting that the antimicrobial activity of ND-mTHPP was afforded by inducing membrane damage. Cytotoxicity against murine embryonic fibroblast cells (MEF) was also evaluated and ND-mTHPP was shown to be noncytotoxic since viability of cells cultured for 24 h in the presence of the nanoconjugate (100 µg/mL) was 78%. Considering the enhanced antibacterial activity and the absence of cytotoxic effect, it is possible to consider the ND-mTHPP nanoconjugate as promising platform for application in antimicrobial photodynamic therapy (aPDT).-
Descrição: dc.descriptionFederal Institute of São Paulo (IFSP)-
Descrição: dc.descriptionNanomaterials and Nanotoxicology Laboratory Institute of Science and Technology Federal University of São Paulo (UNIFESP)-
Descrição: dc.descriptionPeptide Biochemistry Laboratory Institute of Science and Technology Federal University of São Paulo (UNIFESP)-
Descrição: dc.descriptionInstitute of Science and Technology São Paulo State University (UNESP)-
Descrição: dc.descriptionInstitute of Advanced Sea Studies (IEAMAr) São Paulo State University (UNESP)-
Descrição: dc.descriptionInstitute of Medicinal Chemistry Helmholtz Zentrum München-
Descrição: dc.descriptionFundamental Sciences Division Technological Institute of Aeronautics (ITA)-
Descrição: dc.descriptionBioceramics Laboratory Institute of Science and Technology Federal University of São Paulo (UNIFESP)-
Descrição: dc.descriptionSensors and Materials Associated Laboratory National Institute for Space Research (INPE)-
Descrição: dc.descriptionDepartment of Biochemistry Institute of Chemistry University of São Paulo (USP)-
Descrição: dc.descriptionDepartment of Chemical Sciences University of Johannesburg, P.O. Box 17011, Doornfontein-
Descrição: dc.descriptionCentre for Nanomaterials Science Research University of Johannesburg-
Descrição: dc.descriptionInstitute of Science and Technology São Paulo State University (UNESP)-
Descrição: dc.descriptionInstitute of Advanced Sea Studies (IEAMAr) São Paulo State University (UNESP)-
Idioma: dc.languageen-
Relação: dc.relationNanomaterials-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectDrug-resistance-
Palavras-chave: dc.subjectNanoconjugate-
Palavras-chave: dc.subjectPhotodynamic antimicrobial therapy (aPDT)-
Palavras-chave: dc.subjectPhotosensitizers (PS)-
Título: dc.titleDiamond nanoparticles-porphyrin mthpp conjugate as photosensitizing platform: Cytotoxicity and antibacterial activity-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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