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Metadados | Descrição | Idioma |
---|---|---|
Autor(es): dc.contributor | Federal Institute of São Paulo (IFSP) | - |
Autor(es): dc.contributor | Universidade de São Paulo (USP) | - |
Autor(es): dc.contributor | Universidade Estadual Paulista (Unesp) | - |
Autor(es): dc.contributor | Helmholtz Zentrum München | - |
Autor(es): dc.contributor | Technological Institute of Aeronautics (ITA) | - |
Autor(es): dc.contributor | National Institute for Space Research (INPE) | - |
Autor(es): dc.contributor | University of Johannesburg | - |
Autor(es): dc.creator | Hurtado, Carolina Ramos | - |
Autor(es): dc.creator | Hurtado, Gabriela Ramos [UNESP] | - |
Autor(es): dc.creator | de Cena, Gabrielle Lupeti | - |
Autor(es): dc.creator | Queiroz, Rafaela Campos | - |
Autor(es): dc.creator | Silva, Alexandre Vieira | - |
Autor(es): dc.creator | Diniz, Milton Faria | - |
Autor(es): dc.creator | Dos Santos, Verônica Ribeiro | - |
Autor(es): dc.creator | Trava-Airoldi, Vladimir | - |
Autor(es): dc.creator | Baptista, Maurício da Silva | - |
Autor(es): dc.creator | Tsolekile, Ncediwe | - |
Autor(es): dc.creator | Oluwafemi, Oluwatobi Samuel | - |
Autor(es): dc.creator | Conceição, Katia | - |
Autor(es): dc.creator | Tada, Dayane Batista | - |
Data de aceite: dc.date.accessioned | 2022-02-22T00:51:53Z | - |
Data de disponibilização: dc.date.available | 2022-02-22T00:51:53Z | - |
Data de envio: dc.date.issued | 2021-06-25 | - |
Data de envio: dc.date.issued | 2021-06-25 | - |
Data de envio: dc.date.issued | 2021-06-01 | - |
Fonte completa do material: dc.identifier | http://dx.doi.org/10.3390/nano11061393 | - |
Fonte completa do material: dc.identifier | http://hdl.handle.net/11449/207948 | - |
Fonte: dc.identifier.uri | http://educapes.capes.gov.br/handle/11449/207948 | - |
Descrição: dc.description | Conjugation of photosensitizers (PS) with nanoparticles has been largely used as a strategy to stabilize PS in the biological medium resulting in photosensitizing nanoparticles of enhanced photoactivity. Herein, (Meso-5, 10, 15, 20-tetrakis (3-hydroxyphenyl) phorphyryn (mTHPP) was conjugated with diamond nanoparticles (ND) by covalent bond. Nanoconjugate ND-mTHPP showed suitable stability in aqueous suspension with 58 nm of hydrodynamic diameter and Zeta potential of −23 mV. The antibacterial activity of ND-mTHPP was evaluated against Escherichia coli for different incubation times (0–24 h). The optimal activity was observed after 2 h of incubation and irradiation (660 nm; 51 J/cm2 ) performed right after the addition of ND-mTHPP (100 µg/mL) to the bacterial suspension. The inhibitory activity was 56% whereas ampicillin at the same conditions provided only 14% of bacterial growth inhibition. SEM images showed agglomerate of ND-mTHPP adsorbed on the bacterial cell wall, suggesting that the antimicrobial activity of ND-mTHPP was afforded by inducing membrane damage. Cytotoxicity against murine embryonic fibroblast cells (MEF) was also evaluated and ND-mTHPP was shown to be noncytotoxic since viability of cells cultured for 24 h in the presence of the nanoconjugate (100 µg/mL) was 78%. Considering the enhanced antibacterial activity and the absence of cytotoxic effect, it is possible to consider the ND-mTHPP nanoconjugate as promising platform for application in antimicrobial photodynamic therapy (aPDT). | - |
Descrição: dc.description | Federal Institute of São Paulo (IFSP) | - |
Descrição: dc.description | Nanomaterials and Nanotoxicology Laboratory Institute of Science and Technology Federal University of São Paulo (UNIFESP) | - |
Descrição: dc.description | Peptide Biochemistry Laboratory Institute of Science and Technology Federal University of São Paulo (UNIFESP) | - |
Descrição: dc.description | Institute of Science and Technology São Paulo State University (UNESP) | - |
Descrição: dc.description | Institute of Advanced Sea Studies (IEAMAr) São Paulo State University (UNESP) | - |
Descrição: dc.description | Institute of Medicinal Chemistry Helmholtz Zentrum München | - |
Descrição: dc.description | Fundamental Sciences Division Technological Institute of Aeronautics (ITA) | - |
Descrição: dc.description | Bioceramics Laboratory Institute of Science and Technology Federal University of São Paulo (UNIFESP) | - |
Descrição: dc.description | Sensors and Materials Associated Laboratory National Institute for Space Research (INPE) | - |
Descrição: dc.description | Department of Biochemistry Institute of Chemistry University of São Paulo (USP) | - |
Descrição: dc.description | Department of Chemical Sciences University of Johannesburg, P.O. Box 17011, Doornfontein | - |
Descrição: dc.description | Centre for Nanomaterials Science Research University of Johannesburg | - |
Descrição: dc.description | Institute of Science and Technology São Paulo State University (UNESP) | - |
Descrição: dc.description | Institute of Advanced Sea Studies (IEAMAr) São Paulo State University (UNESP) | - |
Idioma: dc.language | en | - |
Relação: dc.relation | Nanomaterials | - |
???dc.source???: dc.source | Scopus | - |
Palavras-chave: dc.subject | Drug-resistance | - |
Palavras-chave: dc.subject | Nanoconjugate | - |
Palavras-chave: dc.subject | Photodynamic antimicrobial therapy (aPDT) | - |
Palavras-chave: dc.subject | Photosensitizers (PS) | - |
Título: dc.title | Diamond nanoparticles-porphyrin mthpp conjugate as photosensitizing platform: Cytotoxicity and antibacterial activity | - |
Tipo de arquivo: dc.type | livro digital | - |
Aparece nas coleções: | Repositório Institucional - Unesp |
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