Chikungunya virus entry is strongly inhibited by phospholipase A2 isolated from the venom of Crotalus durissus terrificus

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Autor(es): dc.contributorUniversidade Federal de Uberlândia (UFU)-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversity of Tartu-
Autor(es): dc.contributorUniversity of Leeds-
Autor(es): dc.creatorSantos, Igor Andrade-
Autor(es): dc.creatorShimizu, Jacqueline Farinha [UNESP]-
Autor(es): dc.creatorde Oliveira, Débora Moraes-
Autor(es): dc.creatorMartins, Daniel Oliveira Silva [UNESP]-
Autor(es): dc.creatorCardoso-Sousa, Léia-
Autor(es): dc.creatorCintra, Adélia Cristina Oliveira-
Autor(es): dc.creatorAquino, Victor Hugo-
Autor(es): dc.creatorSampaio, Suely Vilela-
Autor(es): dc.creatorNicolau-Junior, Nilson-
Autor(es): dc.creatorSabino-Silva, Robinson-
Autor(es): dc.creatorMerits, Andres-
Autor(es): dc.creatorHarris, Mark-
Autor(es): dc.creatorJardim, Ana Carolina Gomes [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:51:51Z-
Data de disponibilização: dc.date.available2022-02-22T00:51:51Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-11-30-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1038/s41598-021-88039-4-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/207935-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/207935-
Descrição: dc.descriptionChikungunya virus (CHIKV) is the etiologic agent of Chikungunya fever, a globally spreading mosquito-borne disease. There is no approved antiviral or vaccine against CHIKV, highlighting an urgent need for novel therapies. In this context, snake venom proteins have demonstrated antiviral activity against several viruses, including arboviruses which are relevant to public health. In particular, the phospholipase A2CB (PLA2CB), a protein isolated from the venom of Crotalus durissus terrificus was previously shown to possess anti-inflammatory, antiparasitic, antibacterial and antiviral activities. In this study, we investigated the multiple effects of PLA2CB on the CHIKV replicative cycle in BHK-21 cells using CHIKV-nanoluc, a marker virus carrying nanoluciferase reporter. The results demonstrated that PLA2CB possess a strong anti-CHIKV activity with a selectivity index of 128. We identified that PLA2CB treatment protected cells against CHIKV infection, strongly impairing virus entry by reducing adsorption and post-attachment stages. Moreover, PLA2CB presented a modest yet significant activity towards post-entry stages of CHIKV replicative cycle. Molecular docking calculations indicated that PLA2CB may interact with CHIKV glycoproteins, mainly with E1 through hydrophobic interactions. In addition, infrared spectroscopy measurements indicated interactions of PLA2CB and CHIKV glycoproteins, corroborating with data from in silico analyses. Collectively, this data demonstrated the multiple antiviral effects of PLA2CB on the CHIKV replicative cycle, and suggest that PLA2CB interacts with CHIKV glycoproteins and that this interaction blocks binding of CHIKV virions to the host cells.-
Descrição: dc.descriptionInstitute of Biomedical Science (ICBIM) Federal University of Uberlândia (UFU), Avenida Amazonas, 4C- Room 216, Umuarama-
Descrição: dc.descriptionInstitute of Biosciences Humanities and Exact Sciences (Ibilce) São Paulo State University (Unesp), Campus São José do Rio Preto-
Descrição: dc.descriptionDepartment of Clinical Toxicological and Bromatological Analyses School of Pharmaceutical Sciences of Ribeirao Preto University of São Paulo (USP)-
Descrição: dc.descriptionInstitute of Biotechnology Federal University of Uberlândia (UFU)-
Descrição: dc.descriptionInstitute of Technology University of Tartu-
Descrição: dc.descriptionFaculty of Biological Sciences and Astbury Centre for Structural Molecular Biology University of Leeds-
Descrição: dc.descriptionInstitute of Biosciences Humanities and Exact Sciences (Ibilce) São Paulo State University (Unesp), Campus São José do Rio Preto-
Idioma: dc.languageen-
Relação: dc.relationScientific Reports-
???dc.source???: dc.sourceScopus-
Título: dc.titleChikungunya virus entry is strongly inhibited by phospholipase A2 isolated from the venom of Crotalus durissus terrificus-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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