Multi-omics analysis provides insights into lignocellulosic biomass degradation by Laetiporus sulphureus ATCC 52600

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual de Campinas (UNICAMP)-
Autor(es): dc.contributorBrazilian Center for Research in Energy and Materials (CNPEM)-
Autor(es): dc.contributorAarhus University-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUniversity of Sorocaba (UNISO)-
Autor(es): dc.contributorSão Paulo Fungal Group-
Autor(es): dc.creatorde Figueiredo, Fernanda Lopes-
Autor(es): dc.creatorde Oliveira, Ana Carolina Piva-
Autor(es): dc.creatorTerrasan, Cesar Rafael Fanchini-
Autor(es): dc.creatorGonçalves, Thiago Augusto-
Autor(es): dc.creatorGerhardt, Jaqueline Aline-
Autor(es): dc.creatorTomazetto, Geizecler-
Autor(es): dc.creatorPersinoti, Gabriela Felix-
Autor(es): dc.creatorRubio, Marcelo Ventura-
Autor(es): dc.creatorPeña, Jennifer Andrea Tamayo-
Autor(es): dc.creatorAraújo, Michelle Fernandes-
Autor(es): dc.creatorde Carvalho Silvello, Maria Augusta-
Autor(es): dc.creatorFranco, Telma Teixeira-
Autor(es): dc.creatorRabelo, Sarita Cândida [UNESP]-
Autor(es): dc.creatorGoldbeck, Rosana-
Autor(es): dc.creatorSquina, Fabio Marcio-
Autor(es): dc.creatorDamasio, André-
Data de aceite: dc.date.accessioned2022-02-22T00:51:51Z-
Data de disponibilização: dc.date.available2022-02-22T00:51:51Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-11-30-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1186/s13068-021-01945-7-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/207933-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/207933-
Descrição: dc.descriptionBackground: Wood-decay basidiomycetes are effective for the degradation of highly lignified and recalcitrant plant substrates. The degradation of lignocellulosic materials by brown-rot strains is carried out by carbohydrate-active enzymes and non-enzymatic Fenton mechanism. Differences in the lignocellulose catabolism among closely related brown rots are not completely understood. Here, a multi-omics approach provided a global understanding of the strategies employed by L. sulphureus ATCC 52600 for lignocellulose degradation. Results: The genome of Laetiporus sulphureus ATCC 52600 was sequenced and phylogenomic analysis supported monophyletic clades for the Order Polyporales and classification of this species within the family Laetiporaceae. Additionally, the plasticity of its metabolism was revealed in growth analysis on mono- and disaccharides, and polysaccharides such as cellulose, hemicelluloses, and polygalacturonic acid. The response of this fungus to the presence of lignocellulosic substrates was analyzed by transcriptomics and proteomics and evidenced the occurrence of an integrated oxidative–hydrolytic metabolism. The transcriptomic profile in response to a short cultivation period on sugarcane bagasse revealed 125 upregulated transcripts, which included CAZymes (redox enzymes and hemicellulases) as well as non-CAZy redox enzymes and genes related to the synthesis of low-molecular-weight compounds. The exoproteome produced in response to extended cultivation time on Avicel, and steam-exploded sugarcane bagasse, sugarcane straw, and Eucalyptus revealed 112 proteins. Contrasting with the mainly oxidative profile observed in the transcriptome, the secretomes showed a diverse hydrolytic repertoire including constitutive cellulases and hemicellulases, in addition to 19 upregulated CAZymes. The secretome induced for 7 days on sugarcane bagasse, representative of the late response, was applied in the saccharification of hydrothermally pretreated grass (sugarcane straw) and softwood (pine) by supplementing a commercial cocktail. Conclusion: This study shows the singularity of L. sulphureus ATCC 52600 compared to other Polyporales brown rots, regarding the presence of cellobiohydrolase and peroxidase class II. The multi-omics analysis reinforces the oxidative–hydrolytic metabolism involved in lignocellulose deconstruction, providing insights into the overall mechanisms as well as specific proteins of each step.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionDepartment of Biochemistry and Tissue Biology Institute of Biology University of Campinas (UNICAMP)-
Descrição: dc.descriptionBrazilian Biorenewables National Laboratory (LNBr) Brazilian Center for Research in Energy and Materials (CNPEM)-
Descrição: dc.descriptionDepartment of Biological and Chemical Engineering (BCE) Aarhus University-
Descrição: dc.descriptionInterdisciplinary Center of Energy Planning (NIPE) University of Campinas (UNICAMP)-
Descrição: dc.descriptionChemical Engineering School University of Campinas (UNICAMP)-
Descrição: dc.descriptionDepartment of Food Engineering Faculty of Food Engineering University of Campinas (UNICAMP)-
Descrição: dc.descriptionDepartment of Bioprocess and Biotechnology College of Agricultural Sciences São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Technological and Environmental Processes University of Sorocaba (UNISO)-
Descrição: dc.descriptionSão Paulo Fungal Group-
Descrição: dc.descriptionDepartment of Bioprocess and Biotechnology College of Agricultural Sciences São Paulo State University (UNESP)-
Descrição: dc.descriptionCAPES: 001-
Descrição: dc.descriptionFAPESP: 15/50590-4-
Descrição: dc.descriptionFAPESP: 15/50612-8-
Descrição: dc.descriptionFAPESP: 17/22669-0-
Descrição: dc.descriptionFAPESP: 20/05784-3-
Descrição: dc.descriptionCNPq: 306279/2020-7-
Descrição: dc.descriptionCNPq: 311457/2020-7-
Descrição: dc.descriptionCNPq: 404654/2018-5-
Descrição: dc.descriptionCNPq: 420392/2018-1-
Descrição: dc.descriptionCNPq: 428527/2018-3-
Idioma: dc.languageen-
Relação: dc.relationBiotechnology for Biofuels-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectBasidiomycetes-
Palavras-chave: dc.subjectBrown-rot-
Palavras-chave: dc.subjectCAZymes-
Palavras-chave: dc.subjectFenton reaction-
Palavras-chave: dc.subjectGenome-
Palavras-chave: dc.subjectProteome-
Palavras-chave: dc.subjectSugarcane by-products-
Palavras-chave: dc.subjectTranscriptome-
Título: dc.titleMulti-omics analysis provides insights into lignocellulosic biomass degradation by Laetiporus sulphureus ATCC 52600-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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