Immunoexpression pattern of autophagy mediators in alveolar bone osteoclasts following estrogen withdrawal in female rats

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Federal de São Paulo (UNIFESP)-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.creatorFlorencio-Silva, Rinaldo-
Autor(es): dc.creatorSasso, Gisela Rodrigues da Silva-
Autor(es): dc.creatorSasso-Cerri, Estela [UNESP]-
Autor(es): dc.creatorSimões, Manuel de Jesus-
Autor(es): dc.creatorCerri, Paulo Sérgio [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:51:46Z-
Data de disponibilização: dc.date.available2022-02-22T00:51:46Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-04-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1007/s10735-020-09953-x-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/207908-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/207908-
Descrição: dc.descriptionIt is known that estrogen deficiency increases osteoclast formation and activity. Autophagy, a cell survival pathway, has been shown to be crucial for osteoclast function. However, little is known about the effects of estrogen depletion on osteoclast autophagy. Here, we evaluated the effects of estrogen deficiency in the immunoexpression of autophagy mediators in alveolar bone osteoclasts of ovariectomized rats. Twelve adult female rats were ovariectomized (OVX-group) or SHAM-operated (SHAM-group). After three weeks, the rats were euthanized and maxillary fragments containing alveolar bone of the first molars were processed for light microscopy or transmission electron microscopy (TEM). Paraffin-sections were subjected to the TRAP method (osteoclast marker) or to the immunohistochemical detections of beclin-1, LC3α, and p62 (autophagy mediators); araldite-sections were processed for TEM. The number of TRAP-positive osteoclasts and the number of immunolabeled-multinucleated cells (MNCs) along the alveolar bone surface of the first molar were computed. The number of TRAP-positive osteoclasts and the number of beclin-1-, LC3α- and p62-immunolabelled osteoclasts were significantly higher in OVX-group than the SHAM-group. MNCs were frequently located juxtaposed to Howship lacunae along the alveolar bone surface, indicating that these cells are osteoclasts. TEM revealed osteoclasts exhibiting autophagosomes. Our data indicate that autophagy plays an important role during estrogen deficiency-induced osteoclastogenesis. Thus, our results contribute to a better understanding on the role of autophagy on osteoclasts under estrogenic deficiency, and reinforce the idea that modulation of autophagy may be a useful tool to inhibit excessive oral bone resorption in post-menopausal women.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionDisciplina de Histologia e Biologia Estrutural Departamento de Morfologia e Genética Escola Paulista de Medicina - EPM Universidade Federal de São Paulo - UNIFESP-
Descrição: dc.descriptionDepartamento de Ginecologia Escola Paulista de Medicina - EPM Universidade Federal de São Paulo - UNIFESP-
Descrição: dc.descriptionAraraquara - Laboratory of Histology and Embryology School of Dentistry São Paulo State University (UNESP)-
Descrição: dc.descriptionAraraquara - Laboratory of Histology and Embryology School of Dentistry São Paulo State University (UNESP)-
Descrição: dc.descriptionFAPESP: 2012/19428-8-
Descrição: dc.descriptionFAPESP: 2012/22666-8-
Formato: dc.format321-333-
Idioma: dc.languageen-
Relação: dc.relationJournal of Molecular Histology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAlveolar bone-
Palavras-chave: dc.subjectAutophagy-
Palavras-chave: dc.subjectEstrogen withdrawal-
Palavras-chave: dc.subjectOsteoclast-
Palavras-chave: dc.subjectRat-
Título: dc.titleImmunoexpression pattern of autophagy mediators in alveolar bone osteoclasts following estrogen withdrawal in female rats-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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