Insulin requires A2B adenosine receptors to modulate the L-arginine/nitric oxide signalling in the human fetoplacental vascular endothelium from late-onset preeclampsia

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorPontificia Universidad Católica de Chile-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversidad de Sevilla-
Autor(es): dc.contributorUniversidad del Bío-Bío-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorFaculty of Medicine and Biomedical Sciences-
Autor(es): dc.creatorSalsoso, Rocío-
Autor(es): dc.creatorMate, Alfonso-
Autor(es): dc.creatorToledo, Fernando-
Autor(es): dc.creatorVázquez, Carmen M.-
Autor(es): dc.creatorSobrevia, Luis [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:51:43Z-
Data de disponibilização: dc.date.available2022-02-22T00:51:43Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2020-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.bbadis.2020.165993-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/207890-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/207890-
Descrição: dc.descriptionLate-onset preeclampsia (LOPE) associates with reduced umbilical vein reactivity and endothelial nitric oxide synthase (eNOS) activity but increased human cationic amino acid (hCAT-1)–mediated L-arginine transport involving A2A adenosine receptor in the fetoplacental unit. This study addresses the A2B adenosine receptor (A2BAR)-mediated response to insulin in the fetoplacental vasculature from LOPE. Umbilical veins and HUVECs were obtained from women with normal (n = 37) or LOPE (n = 35) pregnancies. Umbilical vein rings reactivity to insulin was assayed in the absence or presence of adenosine and MRS-1754 (A2BAR antagonist) in a wire myograph. HUVECs were exposed to insulin, MRS-1754, BAY60–6583 (A2BAR agonist), NECA (general adenosine receptors agonist) or NG-nitro-L-arginine methyl ester (NOS inhibitor). A2BAR, hCAT-1, total and phosphorylated eNOS, Akt and p44/42mapk protein abundance were determined by Western blotting. Insulin receptors A (IR-A) and B (IR-B), eNOS and hCAT-1 mRNA were determined by qPCR. Firefly/Renilla luciferase assay was used to determine −1606 bp SLC7A1 (hCAT-1) promoter activity. L-Citrulline content was measured by HPLC, L-[3H]citrulline formation from L-[3H]arginine by the Citrulline assay, and intracellular cGMP by radioimmunoassay. LOPE-reduced dilation of vein rings to insulin was restored by MRS-1754. HUVECs from LOPE showed higher A2BAR, hCAT-1, and IR-A expression, Akt and p44/42mapk activation, and lower NOS activity. MRS-1754 reversed the LOPE effect on A2BAR, hCAT-1, Akt, and eNOS inhibitory phosphorylation. Insulin reversed the LOPE effect on A2BAR, IR-A and eNOS, but increased hCAT-1–mediated transport. Thus, LOPE alters endothelial function, causing an imbalance in the L-arginine/NO signalling pathway to reduce the umbilical vein dilation to insulin requiring A2BAR activation in HUVECs.-
Descrição: dc.descriptionASCRS Research Foundation-
Descrição: dc.descriptionUniversidad de Sevilla-
Descrição: dc.descriptionFondo Nacional de Desarrollo Científico y Tecnológico-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionJunta de Andalucía-
Descrição: dc.descriptionCellular and Molecular Physiology Laboratory (CMPL) Division of Obstetrics and Gynaecology School of Medicine Faculty of Medicine Pontificia Universidad Católica de Chile-
Descrição: dc.descriptionInstituto do Coracao (InCor) Hospital das Clinicas HCFMUSP Faculdade de Medicina Universidade de Sao Paulo-
Descrição: dc.descriptionDepartamento de Fisiología Facultad de Farmacia Universidad de Sevilla-
Descrição: dc.descriptionDepartment of Basic Sciences Faculty of Sciences Universidad del Bío-Bío-
Descrição: dc.descriptionMedical School (Faculty of Medicine) São Paulo State University (UNESP)-
Descrição: dc.descriptionUniversity of Queensland Centre for Clinical Research (UQCCR) Faculty of Medicine and Biomedical Sciences, QLD-
Descrição: dc.descriptionMedical School (Faculty of Medicine) São Paulo State University (UNESP)-
Descrição: dc.descriptionFondo Nacional de Desarrollo Científico y Tecnológico: 1190316-
Descrição: dc.descriptionFAPESP: 2017/26922–2-
Descrição: dc.descriptionJunta de Andalucía: 2017/440-
Idioma: dc.languageen-
Relação: dc.relationBiochimica et Biophysica Acta - Molecular Basis of Disease-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAdenosine-
Palavras-chave: dc.subjectArginine-
Palavras-chave: dc.subjectEndothelium-
Palavras-chave: dc.subjectInsulin-
Palavras-chave: dc.subjectNitric oxide-
Palavras-chave: dc.subjectPreeclampsia-
Título: dc.titleInsulin requires A2B adenosine receptors to modulate the L-arginine/nitric oxide signalling in the human fetoplacental vascular endothelium from late-onset preeclampsia-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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