Prospecting biomarkers for diagnostic and therapeutic approaches in pythiosis

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorMahidol University-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.creatorChechi, Jéssica Luana [UNESP]-
Autor(es): dc.creatorRotchanapreeda, Tiwa-
Autor(es): dc.creatorda Paz, Giselle Souza [UNESP]-
Autor(es): dc.creatorPrado, Ana Carolina [UNESP]-
Autor(es): dc.creatorOliveira, Alana Lucena [UNESP]-
Autor(es): dc.creatorVieira, José Cavalcante Souza [UNESP]-
Autor(es): dc.creatorBuzalaf, Marília Afonso Rabelo-
Autor(es): dc.creatorRodrigues, Anderson Messias-
Autor(es): dc.creatorDos Santos, Lucilene Delazari [UNESP]-
Autor(es): dc.creatorKrajaejun, Theerapong-
Autor(es): dc.creatorBosco, Sandra de Moraes Gimenes [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:51:39Z-
Data de disponibilização: dc.date.available2022-02-22T00:51:39Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/jof7060423-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/207874-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/207874-
Descrição: dc.descriptionPythiosis, whose etiological agent is the oomycete Pythium insidiosum, is a life-threatening disease that occurs mainly in tropical and subtropical countries, affecting several animal species. It is frequently found in horses in Brazil and humans in Thailand. The disease is difficult to diagnose because the pathogen’s hyphae are often misdiagnosed as mucoromycete fungi in histological sections. Additionally, there is no specific antigen to use for rapid diagnosis, the availability of which could improve the prognosis in different animal species. In this scenario, we investigated which P. insidiosum antigens are recognized by circulating antibodies in horses and humans with pythiosis from Brazil and Thailand, respectively, using 2D immunoblotting followed by mass spectrometry for the identification of antigens. We identified 23 protein spots, 14 recognized by pooled serum from horses and humans. Seven antigens were commonly recognized by both species, such as the heat-shock cognate 70 KDa protein, the heat-shock 70 KDa protein, glucan 1,3-beta-glucosidase, fructose-bisphosphate aldolase, serine/threonine-protein phosphatase, aconitate hydratase, and 14-3-3 protein epsilon. These results demonstrate that there are common antigens recognized by the immune responses of horses and humans, and these antigens may be studied as biomarkers for improving diagnosis and treatment.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionDepartment of Chemical and Biological Sciences Institute of Biosciences São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Pathology Faculty of Medicine Ramathibodi Hospital Mahidol University-
Descrição: dc.descriptionFaculty of Veterinary Medicine and Animal Science (FMVZ) São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Biological Sciences Bauru School of Dentistry University of São Paulo (USP)-
Descrição: dc.descriptionDepartment of Microbiology Immunology and Parasitology Cell Biology Division Federal University of São Paulo (UNIFESP)-
Descrição: dc.descriptionCenter for the Study of Venoms and Venomous Animals (CEVAP) São Paulo State University (UNESP)-
Descrição: dc.descriptionGraduate Program in Tropical Diseases Botucatu Medical School (FMB) São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Chemical and Biological Sciences Institute of Biosciences São Paulo State University (UNESP)-
Descrição: dc.descriptionFaculty of Veterinary Medicine and Animal Science (FMVZ) São Paulo State University (UNESP)-
Descrição: dc.descriptionCenter for the Study of Venoms and Venomous Animals (CEVAP) São Paulo State University (UNESP)-
Descrição: dc.descriptionGraduate Program in Tropical Diseases Botucatu Medical School (FMB) São Paulo State University (UNESP)-
Descrição: dc.descriptionCAPES: 001-
Descrição: dc.descriptionFAPESP: 2016/10804-8-
Descrição: dc.descriptionFAPESP: 2018/08009-0-
Descrição: dc.descriptionFAPESP: 2019/08761-7-
Idioma: dc.languageen-
Relação: dc.relationJournal of Fungi-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAntigens-
Palavras-chave: dc.subjectDiagnosis-
Palavras-chave: dc.subjectImmunoproteomics-
Palavras-chave: dc.subjectPythiosis-
Palavras-chave: dc.subjectPythium insidiosum-
Palavras-chave: dc.subjectTherapy-
Título: dc.titleProspecting biomarkers for diagnostic and therapeutic approaches in pythiosis-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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