Circulating Plasma miRNA and Clinical/Hemodynamic Characteristics Provide Additional Predictive Information About Acute Pulmonary Thromboembolism, Chronic Thromboembolic Pulmonary Hypertension and Idiopathic Pulmonary Hypertension

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Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorCancer Institute of São Paulo (ICESP)-
Autor(es): dc.contributorCentro de Ciências da Saúde-
Autor(es): dc.contributorNational Institute of Science and Technology for Regenerative Medicine-
Autor(es): dc.creatorFabro, Alexandre Todorovic-
Autor(es): dc.creatorMachado-Rugolo, Juliana [UNESP]-
Autor(es): dc.creatorBaldavira, Camila Machado-
Autor(es): dc.creatorPrieto, Tabatha Gutierrez-
Autor(es): dc.creatorFarhat, Cecília-
Autor(es): dc.creatorRotea ManGone, Flavia Regina-
Autor(es): dc.creatorBatah, Sabrina Setembre-
Autor(es): dc.creatorCruvinel, Heloísa Resende-
Autor(es): dc.creatorAldá, Maiara Almeida-
Autor(es): dc.creatorMonteiro, Jhonatas Sirino-
Autor(es): dc.creatorPádua, Adriana Inacio-
Autor(es): dc.creatorMorais, Sirlei Siani-
Autor(es): dc.creatorAntônio de Oliveira, Rogério [UNESP]-
Autor(es): dc.creatorSantos, Marcel Koenigkam-
Autor(es): dc.creatorBaddini-Martinez, José Antônio-
Autor(es): dc.creatorSetubal, João Carlos-
Autor(es): dc.creatorRainho, Claudia Aparecida [UNESP]-
Autor(es): dc.creatorYoo, Hugo Hyung Bok [UNESP]-
Autor(es): dc.creatorSilva, Pedro Leme-
Autor(es): dc.creatorNagai, Maria Aparecida [UNESP]-
Autor(es): dc.creatorCapelozzi, Vera Luiza-
Data de aceite: dc.date.accessioned2022-02-22T00:51:38Z-
Data de disponibilização: dc.date.available2022-02-22T00:51:38Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-05-28-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3389/fphar.2021.648769-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/207868-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/207868-
Descrição: dc.descriptionIdiopathic pulmonary artery hypertension (IPAH), chronic thromboembolic pulmonary hypertension (CTEPH), and acute pulmonary embolism (APTE) are life-threatening cardiopulmonary diseases without specific surgical or medical treatment. Although APTE, CTEPH and IPAH are different pulmonary vascular diseases in terms of clinical presentation, prevalence, pathophysiology and prognosis, the identification of their circulating microRNA (miRNAs) might help in recognizing differences in their outcome evolution and clinical forms. The aim of this study was to describe the APTE, CTEPH, and IPAH-associated miRNAs and to predict their target genes. The target genes of the key differentially expressed miRNAs were analyzed, and functional enrichment analyses were carried out. The miRNAs were detected using RT-PCR. Finally, we incorporated plasma circulating miRNAs in baseline and clinical characteristics of the patients to detect differences between APTE and CTEPH in time of evolution, and differences between CTEPH and IPAH in diseases form. We found five top circulating plasma miRNAs in common with APTE, CTEPH and IPAH assembled in one conglomerate. Among them, miR-let-7i-5p expression was upregulated in APTE and IPAH, while miRNA-320a was upregulated in CTEP and IPAH. The network construction for target genes showed 11 genes regulated by let-7i-5p and 20 genes regulated by miR-320a, all of them regulators of pulmonary arterial adventitial fibroblasts, pulmonary artery endothelial cell, and pulmonary artery smooth muscle cells. AR (androgen receptor), a target gene of hsa-let-7i-5p and has-miR-320a, was enriched in pathways in cancer, whereas PRKCA (Protein Kinase C Alpha), also a target gene of hsa-let-7i-5p and has-miR-320a, was enriched in KEGG pathways, such as pathways in cancer, glioma, and PI3K-Akt signaling pathway. We inferred that CTEPH might be the consequence of abnormal remodeling in APTE, while unbalance between the hyperproliferative and apoptosis-resistant phenotype of pulmonary arterial adventitial fibroblasts, pulmonary artery endothelial cell and pulmonary artery smooth muscle cells in pulmonary artery confer differences in IPAH and CTEPH diseases form. We concluded that the incorporation of plasma circulating let-7i-5p and miRNA-320a in baseline and clinical characteristics of the patients reinforces differences between APTE and CTEPH in outcome evolution, as well as differences between CTEPH and IPAH in diseases form.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ)-
Descrição: dc.descriptionDepartment of Pathology Laboratory of Histomorphometry and Lung Genomics Faculty of Medicine University of São Paulo-
Descrição: dc.descriptionDepartment of Pathology and Legal Medicine Respiratory Medicine Laboratory Ribeirão Preto Medical School University of São Paulo (USP)-
Descrição: dc.descriptionHealth Technology Assessment Center (NATS) Clinical Hospital (HCFMB) Medical School of São Paulo State University (UNESP)-
Descrição: dc.descriptionLaboratory of Molecular Genetics Center for Translational Research in Oncology Cancer Institute of São Paulo (ICESP)-
Descrição: dc.descriptionBioinformatic Laboratory Institute of Chemistry University of São Paulo (USP)-
Descrição: dc.descriptionPulmonary Hypertension Care Center Department of Internal Medicine Ribeirão Preto Medical School University of São Paulo (USP)-
Descrição: dc.descriptionDepartment of Biostatistics Plant Biology Parasitology and Zoology Institute of Biosciences São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Chemical and Biological Sciences Institute of Biosciences São Paulo State University (UNESP)-
Descrição: dc.descriptionPulmonary Hypertension Care Center Department of Internal Medicine Botucatu Medical School São Paulo State University (UNESP)-
Descrição: dc.descriptionLaboratory of Pulmonary Investigation Carlos Chagas Filho Biophysics Institute Federal University of Rio de Janeiro Centro de Ciências da Saúde-
Descrição: dc.descriptionNational Institute of Science and Technology for Regenerative Medicine-
Descrição: dc.descriptionDepartment of Radiology and Oncology Medical School of São Paulo State University (UNESP)-
Descrição: dc.descriptionHealth Technology Assessment Center (NATS) Clinical Hospital (HCFMB) Medical School of São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Biostatistics Plant Biology Parasitology and Zoology Institute of Biosciences São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Chemical and Biological Sciences Institute of Biosciences São Paulo State University (UNESP)-
Descrição: dc.descriptionPulmonary Hypertension Care Center Department of Internal Medicine Botucatu Medical School São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Radiology and Oncology Medical School of São Paulo State University (UNESP)-
Idioma: dc.languageen-
Relação: dc.relationFrontiers in Pharmacology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectblood plasma-
Palavras-chave: dc.subjectmicroRNA-
Palavras-chave: dc.subjectpulmonary hypertension-
Palavras-chave: dc.subjectRNA-sequencing-
Palavras-chave: dc.subjectthromboemboilc disease-
Título: dc.titleCirculating Plasma miRNA and Clinical/Hemodynamic Characteristics Provide Additional Predictive Information About Acute Pulmonary Thromboembolism, Chronic Thromboembolic Pulmonary Hypertension and Idiopathic Pulmonary Hypertension-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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