Extracellular‐vesicle‐based coatings enhance bioactivity of titanium implants—surfev

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorThe University of Sydney-
Autor(es): dc.contributorRoyal Women’s Hospital-
Autor(es): dc.contributorUniversity of Melbourne-
Autor(es): dc.creatorPansani, Taisa Nogueira [UNESP]-
Autor(es): dc.creatorPhan, Thanh Huyen-
Autor(es): dc.creatorLei, Qingyu-
Autor(es): dc.creatorKondyurin, Alexey-
Autor(es): dc.creatorKalionis, Bill-
Autor(es): dc.creatorChrzanowski, Wojciech-
Data de aceite: dc.date.accessioned2022-02-22T00:51:25Z-
Data de disponibilização: dc.date.available2022-02-22T00:51:25Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/nano11061445-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/207789-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/207789-
Descrição: dc.descriptionExtracellular vesicles (EVs) are nanoparticles released by cells that contain a multitude of biomolecules, which act synergistically to signal multiple cell types. EVs are ideal candidates for promoting tissue growth and regeneration. The tissue regenerative potential of EVs raises the tan-talizing possibility that immobilizing EVs on implant surfaces could potentially generate highly bioactive and cell‐instructive surfaces that would enhance implant integration into the body. Such surfaces could address a critical limitation of current implants, which do not promote bone tissue formation or bond bone. Here, we developed bioactive titanium surface coatings (SurfEV) using two types of EVs: secreted by decidual mesenchymal stem cells (DEVs) and isolated from fermented papaya fluid (PEVs). For each EV type, we determined the size, morphology, and molecular com-position. High concentrations of DEVs enhanced cell proliferation, wound closure, and migration distance of osteoblasts. In contrast, the cell proliferation and wound closure decreased with increas-ing concentration of PEVs. DEVs enhanced Ca/P deposition on the titanium surface, which suggests improvement in bone bonding ability of the implant (i.e., osteointegration). EVs also increased pro-duction of Ca and P by osteoblasts and promoted the deposition of mineral phase, which suggests EVs play key roles in cell mineralization. We also found that DEVs stimulated the secretion of sec-ondary EVs observed by the presence of protruding structures on the cell membrane. We concluded that, by functionalizing implant surfaces with specialized EVs, we will be able to enhance implant osteointegration by improving hydroxyapatite formation directly at the surface and potentially cir-cumvent aseptic loosening of implants.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionDepartment of Dental Materials and Prosthodontics Araraquara School of Dentistry São Paulo State University (UNESP)-
Descrição: dc.descriptionFaculty of Medicine and Health Sydney School of Pharmacy Sydney Nano Institute The University of Sydney, Camperdown-
Descrição: dc.descriptionSydney School of Physics The University of Sydney, Camperdown-
Descrição: dc.descriptionDepartment of Maternal‐Fetal Medicine Pregnancy Royal Women’s Hospital, Parkville-
Descrição: dc.descriptionDepartment of Obstetrics and Gynecology University of Melbourne, Parkville-
Descrição: dc.descriptionDepartment of Dental Materials and Prosthodontics Araraquara School of Dentistry São Paulo State University (UNESP)-
Descrição: dc.descriptionFAPESP: 2018/05258‐0-
Idioma: dc.languageen-
Relação: dc.relationNanomaterials-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectBioactivity-
Palavras-chave: dc.subjectExtracellular vesicle-
Palavras-chave: dc.subjectImplants-
Palavras-chave: dc.subjectOsteoblasts-
Palavras-chave: dc.subjectOsteointegration-
Palavras-chave: dc.subjectTitanium-
Título: dc.titleExtracellular‐vesicle‐based coatings enhance bioactivity of titanium implants—surfev-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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