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Metadados | Descrição | Idioma |
---|---|---|
Autor(es): dc.contributor | Universidade Estadual Paulista (Unesp) | - |
Autor(es): dc.contributor | University of Calgary | - |
Autor(es): dc.contributor | Rodovia Geraldo Silva Nascimento | - |
Autor(es): dc.creator | Rodrigues, Maira S. [UNESP] | - |
Autor(es): dc.creator | Fallah, Hamideh P. | - |
Autor(es): dc.creator | Zanardini, Maya | - |
Autor(es): dc.creator | Malafaia, Guilherme [UNESP] | - |
Autor(es): dc.creator | Habibi, Hamid R. | - |
Autor(es): dc.creator | Nóbrega, Rafael H. [UNESP] | - |
Data de aceite: dc.date.accessioned | 2022-02-22T00:51:25Z | - |
Data de disponibilização: dc.date.available | 2022-02-22T00:51:25Z | - |
Data de envio: dc.date.issued | 2021-06-25 | - |
Data de envio: dc.date.issued | 2021-06-25 | - |
Data de envio: dc.date.issued | 2021-07-15 | - |
Fonte completa do material: dc.identifier | http://dx.doi.org/10.1016/j.mce.2021.111331 | - |
Fonte completa do material: dc.identifier | http://hdl.handle.net/11449/207784 | - |
Fonte: dc.identifier.uri | http://educapes.capes.gov.br/handle/11449/207784 | - |
Descrição: dc.description | Reproduction is under multifactorial control of neurohormones, pituitary gonadotropins, as well as of local gonadal signaling systems including sex steroids, growth factors and non-coding RNAs. Among the factors, gonadotropin-inhibitory hormone (Gnih) is a novel RFamide neuropeptide which directly modulates gonadotropin synthesis and release from pituitary, and in the gonads, Gnih mediated inhibitory actions on gonadotropin response of zebrafish spermatogenesis. Thyroid hormones are peripheral hormones which are also known to interact with reproductive axis, in particular, regulating testicular development and function. This study investigated the interaction between Gnih and thyroid hormones in zebrafish spermatogenesis using in vivo and ex vivo approaches. Three experimental groups were established: “control” (non-treated fish), “methimazole” and “methimazole + T4”. Fish were exposed to goitrogen methimazole for 3 weeks; T4 (100 μg/L) was added in the water from the second week only in the “reversal treatment” group. After exposure, testes were dissected out and immediately incubated in Leibovitz's L-15 culture medium containing hCG, Gnih or hCG + Gnih for 7 days. Germ cell cysts and haploid cell population were evaluated by histomorphometry and flow cytometry, respectively. Our results showed that hypothyroidism affected germ cell development in basal and gonadotropin-induced spermatogenesis, in particular, meiosis and spermiogenesis. Hypothyroid testes showed lower amount of spermatozoa, and decreased potency of hCG. We also showed that goitrogen treatment nullified the inhibitory actions of Gnih on the gonadotropin-induced spermatogenesis. This study provided evidences that thyroid hormones are important regulatory factors for hCG- and Gnih-mediated functions in zebrafish spermatogenesis. | - |
Descrição: dc.description | Natural Sciences and Engineering Research Council of Canada | - |
Descrição: dc.description | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) | - |
Descrição: dc.description | Aquaculture Program (CAUNESP) São Paulo State University (UNESP), 14884-900, Jaboticabal | - |
Descrição: dc.description | Department of Biological Sciences University of Calgary, 2500 University Drive NW | - |
Descrição: dc.description | Reproductive and Molecular Biology Group Department of Structural and Functional Biology Institute of Biosciences São Paulo State University (UNESP), 18618-970, Botucatu | - |
Descrição: dc.description | Biological Research Laboratory Goiano Federal Institution Urata Campus Rodovia Geraldo Silva Nascimento, 2,5 km, Zona Rural, Urutaí | - |
Descrição: dc.description | Aquaculture Program (CAUNESP) São Paulo State University (UNESP), 14884-900, Jaboticabal | - |
Descrição: dc.description | Reproductive and Molecular Biology Group Department of Structural and Functional Biology Institute of Biosciences São Paulo State University (UNESP), 18618-970, Botucatu | - |
Descrição: dc.description | Natural Sciences and Engineering Research Council of Canada: 1021837 | - |
Descrição: dc.description | FAPESP: 2014/07620–7 | - |
Descrição: dc.description | FAPESP: 2017/15793–7 | - |
Descrição: dc.description | FAPESP: 2018/15319–6 | - |
Idioma: dc.language | en | - |
Relação: dc.relation | Molecular and Cellular Endocrinology | - |
???dc.source???: dc.source | Scopus | - |
Palavras-chave: dc.subject | Gnih | - |
Palavras-chave: dc.subject | hCG | - |
Palavras-chave: dc.subject | Hypothyroidism | - |
Palavras-chave: dc.subject | Spermatogenesis | - |
Palavras-chave: dc.subject | Thyroid hormones | - |
Palavras-chave: dc.subject | Zebrafish | - |
Título: dc.title | Interaction between thyroid hormones and gonadotropin inhibitory hormone in ex vivo culture of zebrafish testis: An approach to study multifactorial control of spermatogenesis | - |
Tipo de arquivo: dc.type | livro digital | - |
Aparece nas coleções: | Repositório Institucional - Unesp |
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