Alginate-based microparticles coated with HPMCP/AS cellulose-derivatives enable the Ctx(Ile21)-Ha antimicrobial peptide application as a feed additive

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Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorBiological Institute-
Autor(es): dc.contributorUniversity of Birmingham-
Autor(es): dc.creatorRoque-Borda, Cesar Augusto [UNESP]-
Autor(es): dc.creatorSilva, Hanyeny Raiely Leite [UNESP]-
Autor(es): dc.creatorCrusca Junior, Edson [UNESP]-
Autor(es): dc.creatorSerafim, Jéssica Aparecida [UNESP]-
Autor(es): dc.creatorMeneguin, Andréia Bagliotti [UNESP]-
Autor(es): dc.creatorChorilli, Marlus [UNESP]-
Autor(es): dc.creatorMacedo, Wagner Costa [UNESP]-
Autor(es): dc.creatorTeixeira, Silvio Rainho [UNESP]-
Autor(es): dc.creatorGuastalli, Elisabete Aparecida Lopes-
Autor(es): dc.creatorSoares, Nilce Maria-
Autor(es): dc.creatorBlair, Jessica M.A.-
Autor(es): dc.creatorPikramenou, Zoe-
Autor(es): dc.creatorVicente, Eduardo Festozo [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:51:15Z-
Data de disponibilização: dc.date.available2022-02-22T00:51:15Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-07-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.ijbiomac.2021.05.011-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/207738-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/207738-
Descrição: dc.descriptionMicroencapsulation is a potential biotechnological tool, which can overcome antimicrobial peptides (AMP) instabilities and reduce toxic side effects. Thus, this study evaluates the antibacterial activities of the Ctx(Ile21)-Ha AMP against multidrug-resistant (MDR) and non-resistant bacteria and develop and characterize peptide-loaded microparticles coated with the enteric polymers hydroxypropylmethylcellulose acetate succinate (HPMCAS) and hydroxypropylmethylcellulose phthalate (HPMCP). Ctx(Ile21)-Ha was obtained by solid phase peptide synthesis (SPPS) method, purified and characterized by HPLC and Mass Spectrometry. The peptide exhibited potent antibiotic activities against Salmonella enteritidis, Salmonella typhimurium, Pseudomonas aeruginosa (MDR), Acinetobacter baumannii (MDR), and Staphylococcus aureus (MDR). Ctx(Ile21)-Ha microencapsulation was performed by ionic gelation with high efficiency, maintaining the physical-chemical stability. Ctx(Ile21)-Ha coated-microparticles were characterized by DSC, TGA, FTIR-Raman, XRD and SEM. Hemolytic activity assay demonstrated that hemolysis was decreased up to 95% compared to single molecule. In addition, in vitro release control profile simulating different portions of gastrointestinal tract was performed and showed the microcapsules' ability to protect the peptide and release it in the intestine, aiming pathogen's location, mainly by Salmonella sp. Therefore, use of microencapsulated Ctx(Ile21)-Ha can be allowed as an antimicrobial controller in monogastric animal production as an oral feed additive (antimicrobial controller), being a valuable option for molecules with low therapeutic indexes or high hemolytic rates.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionSão Paulo State University (Unesp) School of Agricultural and Veterinarian Sciences, Jaboticabal-
Descrição: dc.descriptionSão Paulo State University (Unesp) Institute of Chemistry, Araraquara-
Descrição: dc.descriptionSão Paulo State University (Unesp) School of Sciences and Engineering, Tupã-
Descrição: dc.descriptionSão Paulo State University (Unesp) School of Pharmaceutical Sciences, Araraquara-
Descrição: dc.descriptionSão Paulo State University (Unesp) School of Technology and Sciences, Presidente Prudente-
Descrição: dc.descriptionPoultry Health Specialized Laboratory Biological Institute, Bastos-
Descrição: dc.descriptionInstitute of Microbiology and Infection University of Birmingham-
Descrição: dc.descriptionSchool of Chemistry University of Birmingham-
Descrição: dc.descriptionSão Paulo State University (Unesp) School of Agricultural and Veterinarian Sciences, Jaboticabal-
Descrição: dc.descriptionSão Paulo State University (Unesp) Institute of Chemistry, Araraquara-
Descrição: dc.descriptionSão Paulo State University (Unesp) School of Sciences and Engineering, Tupã-
Descrição: dc.descriptionSão Paulo State University (Unesp) School of Pharmaceutical Sciences, Araraquara-
Descrição: dc.descriptionSão Paulo State University (Unesp) School of Technology and Sciences, Presidente Prudente-
Descrição: dc.descriptionFAPESP: 2016/00446-7-
Formato: dc.format1236-1247-
Idioma: dc.languageen-
Relação: dc.relationInternational Journal of Biological Macromolecules-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAMP-
Palavras-chave: dc.subjectAntibacterial activity-
Palavras-chave: dc.subjectMicrocapsules-
Título: dc.titleAlginate-based microparticles coated with HPMCP/AS cellulose-derivatives enable the Ctx(Ile21)-Ha antimicrobial peptide application as a feed additive-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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