Atenção: Todas as denúncias são sigilosas e sua identidade será preservada.
Os campos nome e e-mail são de preenchimento opcional
Metadados | Descrição | Idioma |
---|---|---|
Autor(es): dc.contributor | Universidade Estadual Paulista (Unesp) | - |
Autor(es): dc.contributor | Biological Institute | - |
Autor(es): dc.contributor | University of Birmingham | - |
Autor(es): dc.creator | Roque-Borda, Cesar Augusto [UNESP] | - |
Autor(es): dc.creator | Silva, Hanyeny Raiely Leite [UNESP] | - |
Autor(es): dc.creator | Crusca Junior, Edson [UNESP] | - |
Autor(es): dc.creator | Serafim, Jéssica Aparecida [UNESP] | - |
Autor(es): dc.creator | Meneguin, Andréia Bagliotti [UNESP] | - |
Autor(es): dc.creator | Chorilli, Marlus [UNESP] | - |
Autor(es): dc.creator | Macedo, Wagner Costa [UNESP] | - |
Autor(es): dc.creator | Teixeira, Silvio Rainho [UNESP] | - |
Autor(es): dc.creator | Guastalli, Elisabete Aparecida Lopes | - |
Autor(es): dc.creator | Soares, Nilce Maria | - |
Autor(es): dc.creator | Blair, Jessica M.A. | - |
Autor(es): dc.creator | Pikramenou, Zoe | - |
Autor(es): dc.creator | Vicente, Eduardo Festozo [UNESP] | - |
Data de aceite: dc.date.accessioned | 2022-02-22T00:51:15Z | - |
Data de disponibilização: dc.date.available | 2022-02-22T00:51:15Z | - |
Data de envio: dc.date.issued | 2021-06-25 | - |
Data de envio: dc.date.issued | 2021-06-25 | - |
Data de envio: dc.date.issued | 2021-07-31 | - |
Fonte completa do material: dc.identifier | http://dx.doi.org/10.1016/j.ijbiomac.2021.05.011 | - |
Fonte completa do material: dc.identifier | http://hdl.handle.net/11449/207738 | - |
Fonte: dc.identifier.uri | http://educapes.capes.gov.br/handle/11449/207738 | - |
Descrição: dc.description | Microencapsulation is a potential biotechnological tool, which can overcome antimicrobial peptides (AMP) instabilities and reduce toxic side effects. Thus, this study evaluates the antibacterial activities of the Ctx(Ile21)-Ha AMP against multidrug-resistant (MDR) and non-resistant bacteria and develop and characterize peptide-loaded microparticles coated with the enteric polymers hydroxypropylmethylcellulose acetate succinate (HPMCAS) and hydroxypropylmethylcellulose phthalate (HPMCP). Ctx(Ile21)-Ha was obtained by solid phase peptide synthesis (SPPS) method, purified and characterized by HPLC and Mass Spectrometry. The peptide exhibited potent antibiotic activities against Salmonella enteritidis, Salmonella typhimurium, Pseudomonas aeruginosa (MDR), Acinetobacter baumannii (MDR), and Staphylococcus aureus (MDR). Ctx(Ile21)-Ha microencapsulation was performed by ionic gelation with high efficiency, maintaining the physical-chemical stability. Ctx(Ile21)-Ha coated-microparticles were characterized by DSC, TGA, FTIR-Raman, XRD and SEM. Hemolytic activity assay demonstrated that hemolysis was decreased up to 95% compared to single molecule. In addition, in vitro release control profile simulating different portions of gastrointestinal tract was performed and showed the microcapsules' ability to protect the peptide and release it in the intestine, aiming pathogen's location, mainly by Salmonella sp. Therefore, use of microencapsulated Ctx(Ile21)-Ha can be allowed as an antimicrobial controller in monogastric animal production as an oral feed additive (antimicrobial controller), being a valuable option for molecules with low therapeutic indexes or high hemolytic rates. | - |
Descrição: dc.description | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) | - |
Descrição: dc.description | São Paulo State University (Unesp) School of Agricultural and Veterinarian Sciences, Jaboticabal | - |
Descrição: dc.description | São Paulo State University (Unesp) Institute of Chemistry, Araraquara | - |
Descrição: dc.description | São Paulo State University (Unesp) School of Sciences and Engineering, Tupã | - |
Descrição: dc.description | São Paulo State University (Unesp) School of Pharmaceutical Sciences, Araraquara | - |
Descrição: dc.description | São Paulo State University (Unesp) School of Technology and Sciences, Presidente Prudente | - |
Descrição: dc.description | Poultry Health Specialized Laboratory Biological Institute, Bastos | - |
Descrição: dc.description | Institute of Microbiology and Infection University of Birmingham | - |
Descrição: dc.description | School of Chemistry University of Birmingham | - |
Descrição: dc.description | São Paulo State University (Unesp) School of Agricultural and Veterinarian Sciences, Jaboticabal | - |
Descrição: dc.description | São Paulo State University (Unesp) Institute of Chemistry, Araraquara | - |
Descrição: dc.description | São Paulo State University (Unesp) School of Sciences and Engineering, Tupã | - |
Descrição: dc.description | São Paulo State University (Unesp) School of Pharmaceutical Sciences, Araraquara | - |
Descrição: dc.description | São Paulo State University (Unesp) School of Technology and Sciences, Presidente Prudente | - |
Descrição: dc.description | FAPESP: 2016/00446-7 | - |
Formato: dc.format | 1236-1247 | - |
Idioma: dc.language | en | - |
Relação: dc.relation | International Journal of Biological Macromolecules | - |
???dc.source???: dc.source | Scopus | - |
Palavras-chave: dc.subject | AMP | - |
Palavras-chave: dc.subject | Antibacterial activity | - |
Palavras-chave: dc.subject | Microcapsules | - |
Título: dc.title | Alginate-based microparticles coated with HPMCP/AS cellulose-derivatives enable the Ctx(Ile21)-Ha antimicrobial peptide application as a feed additive | - |
Tipo de arquivo: dc.type | livro digital | - |
Aparece nas coleções: | Repositório Institucional - Unesp |
O Portal eduCAPES é oferecido ao usuário, condicionado à aceitação dos termos, condições e avisos contidos aqui e sem modificações. A CAPES poderá modificar o conteúdo ou formato deste site ou acabar com a sua operação ou suas ferramentas a seu critério único e sem aviso prévio. Ao acessar este portal, você, usuário pessoa física ou jurídica, se declara compreender e aceitar as condições aqui estabelecidas, da seguinte forma: