Differential expression of miRNAs in the presence of B chromosome in the cichlid fish Astatotilapia latifasciata

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Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUniversity of Ninth of July (UNINOVE)-
Autor(es): dc.contributorUniversity of Bonn-
Autor(es): dc.creatorNascimento-Oliveira, Jordana Inácio [UNESP]-
Autor(es): dc.creatorFantinatti, Bruno Evaristo Almeida-
Autor(es): dc.creatorWolf, Ivan Rodrigo [UNESP]-
Autor(es): dc.creatorCardoso, Adauto Lima [UNESP]-
Autor(es): dc.creatorRamos, Erica [UNESP]-
Autor(es): dc.creatorRieder, Nathalie-
Autor(es): dc.creatorde Oliveira, Rogerio [UNESP]-
Autor(es): dc.creatorMartins, Cesar [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:51:14Z-
Data de disponibilização: dc.date.available2022-02-22T00:51:14Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-11-30-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1186/s12864-021-07651-w-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/207728-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/207728-
Descrição: dc.descriptionBackground: B chromosomes (Bs) are extra elements observed in diverse eukaryotes, including animals, plants and fungi. Although Bs were first identified a century ago and have been studied in hundreds of species, their biology is still enigmatic. Recent advances in omics and big data technologies are revolutionizing the B biology field. These advances allow analyses of DNA, RNA, proteins and the construction of interactive networks for understanding the B composition and behavior in the cell. Several genes have been detected on the B chromosomes, although the interaction of B sequences and the normal genome remains poorly understood. Results: We identified 727 miRNA precursors in the A. latifasciata genome, 66% which were novel predicted sequences that had not been identified before. We were able to report the A. latifasciata-specific miRNAs and common miRNAs identified in other fish species. For the samples carrying the B chromosome (B+), we identified 104 differentially expressed (DE) miRNAs that are down or upregulated compared to samples without B chromosome (B−) (p < 0.05). These miRNAs share common targets in the brain, muscle and gonads. These targets were used to construct a protein-protein-miRNA network showing the high interaction between the targets of differentially expressed miRNAs in the B+ chromosome samples. Among the DE-miRNA targets there are protein-coding genes reported for the B chromosome that are present in the protein-protein-miRNA network. Additionally, Gene Ontology (GO) terms related to nuclear matrix organization and response to stimulus are exclusive to DE miRNA targets of B+ samples. Conclusions: This study is the first to report the connection of B chromosomes and miRNAs in a vertebrate species. We observed that the B chromosome impacts the miRNAs expression in several tissues and these miRNAs target several mRNAs involved with important biological processes.-
Descrição: dc.descriptionDepartment of Structural and Functional Biology Institute of Bioscience at Botucatu São Paulo State University (UNESP)-
Descrição: dc.descriptionMedical School University of Ninth of July (UNINOVE)-
Descrição: dc.descriptionFaculty of Mathematics and Natural Sciences University of Bonn-
Descrição: dc.descriptionDepartment of Biostatistics Plant Biology Parasitology and Zoology Institute of Bioscience at Botucatu São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Structural and Functional Biology Institute of Bioscience at Botucatu São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Biostatistics Plant Biology Parasitology and Zoology Institute of Bioscience at Botucatu São Paulo State University (UNESP)-
Idioma: dc.languageen-
Relação: dc.relationBMC Genomics-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectCichlid-
Palavras-chave: dc.subjectFish-
Palavras-chave: dc.subjectGenomic-
Palavras-chave: dc.subjectRNA-seq-
Palavras-chave: dc.subjectSelfish element-
Palavras-chave: dc.subjectSmall noncoding RNAs-
Palavras-chave: dc.subjectSupernumerary chromosome-
Palavras-chave: dc.subjectTeleost-
Palavras-chave: dc.subjectTranscriptome-
Título: dc.titleDifferential expression of miRNAs in the presence of B chromosome in the cichlid fish Astatotilapia latifasciata-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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