Evaluation of the Freezability of the Bovine Epididymis Tail Sperm with the Addition of Antioxidants

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Autor(es): dc.contributorParanaense University-
Autor(es): dc.contributorSanto Amaro University-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUniversidade Estadual de Londrina (UEL)-
Autor(es): dc.creatorPassamani da Cruz, G.-
Autor(es): dc.creatorZanfrilli Dos Santos, A. P.-
Autor(es): dc.creatorde Freitas Guaitolini, C. R.-
Autor(es): dc.creatorTramontin, M. L.D.-
Autor(es): dc.creatorRigoto, R. P.-
Autor(es): dc.creatorCrespilho, A. M.-
Autor(es): dc.creatorDell'Aqua, C. P.F. [UNESP]-
Autor(es): dc.creatorMartins, M. I.M.-
Autor(es): dc.creatorMarques, A. B.-
Autor(es): dc.creatorHidalgo, M. M.T.-
Autor(es): dc.creatorSestari, D. A.O.-
Autor(es): dc.creatorMagalhaes, R.-
Autor(es): dc.creatorMaziero, R. R.D.-
Data de aceite: dc.date.accessioned2022-02-22T00:51:13Z-
Data de disponibilização: dc.date.available2022-02-22T00:51:13Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-03-01-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/207726-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/207726-
Descrição: dc.descriptionBACKGROUND: The cryopreservation and recovery of epididymis tail sperm is an important biotechnology dependent on the composition of the freezing medium. OBJETIVE: To evaluate the effect of melatonin, added to commercial freezing medium extender, on the kinetics and viability of bovine epididymis tail sperm. MATERIAL AND METHODS: Five routines were performed, each consisting of eight epididymis and the structures were sliced onto a glass plate containing a commercial diluting medium for Botubov. The samples were divided into four groups, with 80 x 106 spermatozoa per mL. Group 1: samples diluted in Botubov. Group 2: samples centrifuged (600 g, 10 min), and the pellet re-suspended in Botubov. Group 3, samples diluted in Botubov containing 100 pM melatonin. Group 4: samples centrifuged (600 g, 10 min) and the pellet resuspended in Botubov with 100 pM melatonin. The samples were transferred to 0.5 mL straws at 40 x 106 viable spermatozoa, stabilized at 5º C for 4 h, transferred to liquid nitrogen vapour for 20 min, dipped in liquid nitrogen and stored in a cryogenic cylinder. After thawing (46ºC, 15s), sperm kinetics and viability parameters were evaluated. RESULTS: There was no difference in the parameters of total motility (MT, %), progressive motility (MP, %), progressive linear velocity (VSL, µm/s), curvilinear velocity (VCL, µm/s), linearity (LIN, %), spermatozoa with rapid movement (RAP, %) and level of intact plasma membranes and acrosome (IPMA, %) among the groups studied. However, a difference was observed between the routines performed. CONCLUSION: The protocol for freezing bovine epididymis tail sperm is applicable; however, there is an influence of the epididymis used, for the best efficacy of this biotechnology.-
Descrição: dc.descriptionParanaense University-
Descrição: dc.descriptionSanto Amaro University, Sao Paulo/SP-
Descrição: dc.descriptionDepartment of Radiology and Animal Reproduction FMVZ/UNESP-
Descrição: dc.descriptionLondrina State University UEL-
Descrição: dc.descriptionDepartment of Radiology and Animal Reproduction FMVZ/UNESP-
Formato: dc.format81-86-
Idioma: dc.languageen-
Relação: dc.relationCryo letters-
???dc.source???: dc.sourceScopus-
Título: dc.titleEvaluation of the Freezability of the Bovine Epididymis Tail Sperm with the Addition of Antioxidants-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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