Systematic approach to identify novel antimicrobial and antibiofilm molecules from plants' extracts and fractions to prevent dental caries

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.creatorRibeiro, Sabrina M. [UNESP]-
Autor(es): dc.creatorFratucelli, Érick D. O. [UNESP]-
Autor(es): dc.creatorFernandes, Júlia M. [UNESP]-
Autor(es): dc.creatorBueno, Paula C. P. [UNESP]-
Autor(es): dc.creatorCavalheiro, Alberto José [UNESP]-
Autor(es): dc.creatorKlein, Marlise I. [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:51:12Z-
Data de disponibilização: dc.date.available2022-02-22T00:51:12Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-03-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3791/61773-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/207720-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/207720-
Descrição: dc.descriptionNatural products provide structurally different substances, with a myriad of biological activities. However, the identification and isolation of active compounds from plants are challenging because of the complex plant matrix and time-consuming isolation and identification procedures. Therefore, a stepwise approach for screening natural compounds from plants, including the isolation and identification of potentially active molecules, is presented. It includes the collection of the plant material; preparation and fractionation of crude extracts; chromatography and spectrometry (UHPLC-DAD-HRMS and NMR) approaches for analysis and compounds identification; bioassays (antimicrobial and antibiofilm activities; bacterial adhesion strength to the salivary pellicle and initial glucan matrix treated with selected treatments); and data analysis. The model is simple, reproducible, and allows high-throughput screening of multiple compounds, concentrations, and treatment steps can be consistently controlled. The data obtained provide the foundation for future studies, including formulations with the most active extracts and/or fractions, isolation of molecules, modeling molecules to specific targets in microbial cells and biofilms. For example, one target to control cariogenic biofilm is to inhibit the activity of Streptococcus mutans glucosyltransferases that synthesize the extracellular matrix’ glucans. The inhibition of those enzymes prevents the biofilm build-up, decreasing its virulence.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionDepartment of Dental Materials and Prosthodontics School of Dentistry São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Organic Chemistry Institute of Chemistry São Paulo State University (UNESP)-
Descrição: dc.descriptionFaculty of Pharmaceutical Sciences of Ribeiraão Preto (FCFRP-USP) Department of Physics and Chemistry University of São Paulo-
Descrição: dc.descriptionDepartment of Dental Materials and Prosthodontics School of Dentistry São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Organic Chemistry Institute of Chemistry São Paulo State University (UNESP)-
Descrição: dc.descriptionFAPESP: 2011/21440–3-
Descrição: dc.descriptionFAPESP: 2012/21921– 4-
Descrição: dc.descriptionFAPESP: 2013/07600–3-
Descrição: dc.descriptionFAPESP: 2014/50926–0-
Descrição: dc.descriptionFAPESP: 2017/07408–6-
Descrição: dc.descriptionFAPESP: 2019/23175-7-
Descrição: dc.descriptionFAPESP: 465637/2014–0-
Idioma: dc.languageen-
Relação: dc.relationJournal of Visualized Experiments-
???dc.source???: dc.sourceScopus-
Título: dc.titleSystematic approach to identify novel antimicrobial and antibiofilm molecules from plants' extracts and fractions to prevent dental caries-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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