Large-Scale Recombinant Production of the SARS-CoV-2 Proteome for High-Throughput and Structural Biology Applications

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Autor(es): dc.contributorGoethe University Frankfurt-
Autor(es): dc.contributorCNRS/Lyon University-
Autor(es): dc.contributorETH Zurich-
Autor(es): dc.contributorFondazione Ri.MED-
Autor(es): dc.contributorFederal University of Rio de Janeiro-
Autor(es): dc.contributorCaxias Federal University of Rio de Janeiro-
Autor(es): dc.contributorUniversity of Wisconsin-Madison-
Autor(es): dc.contributorUniversity of California-
Autor(es): dc.contributorNIDDK-
Autor(es): dc.contributorIBS-
Autor(es): dc.contributorLatvian Institute of Organic Synthesis-
Autor(es): dc.contributorLeibniz University Hannover-
Autor(es): dc.contributorHelmholtz Centre for Infection Research-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorBuchmann Institute for Molecular Life Sciences-
Autor(es): dc.contributorUniversity of New Hampshire-
Autor(es): dc.contributorUniversity of Florence-
Autor(es): dc.contributorUniversity of Patras-
Autor(es): dc.contributorOswaldo Cruz Foundation (FIOCRUZ)-
Autor(es): dc.contributorUConn Health-
Autor(es): dc.contributorSignals GmbH Co. KG-
Autor(es): dc.contributorLeibniz Institute on Aging—Fritz Lipmann Institute (FLI)-
Autor(es): dc.contributorLatvian Biomedical Research and Study Centre-
Autor(es): dc.contributorSpanish National Research Council (CSIC)-
Autor(es): dc.contributorKarlsruhe Institute of Technology-
Autor(es): dc.contributorTechnical University of Darmstadt-
Autor(es): dc.contributorMartin Luther University Halle-Wittenberg-
Autor(es): dc.creatorAltincekic, Nadide-
Autor(es): dc.creatorKorn, Sophie Marianne-
Autor(es): dc.creatorQureshi, Nusrat Shahin-
Autor(es): dc.creatorDujardin, Marie-
Autor(es): dc.creatorNinot-Pedrosa, Martí-
Autor(es): dc.creatorAbele, Rupert-
Autor(es): dc.creatorAbi Saad, Marie Jose-
Autor(es): dc.creatorAlfano, Caterina-
Autor(es): dc.creatorAlmeida, Fabio C. L.-
Autor(es): dc.creatorAlshamleh, Islam-
Autor(es): dc.creatorde Amorim, Gisele Cardoso-
Autor(es): dc.creatorAnderson, Thomas K.-
Autor(es): dc.creatorAnobom, Cristiane D.-
Autor(es): dc.creatorAnorma, Chelsea-
Autor(es): dc.creatorBains, Jasleen Kaur-
Autor(es): dc.creatorBax, Adriaan-
Autor(es): dc.creatorBlackledge, Martin-
Autor(es): dc.creatorBlechar, Julius-
Autor(es): dc.creatorBöckmann, Anja-
Autor(es): dc.creatorBrigandat, Louis-
Autor(es): dc.creatorBula, Anna-
Autor(es): dc.creatorBütikofer, Matthias-
Autor(es): dc.creatorCamacho-Zarco, Aldo R.-
Autor(es): dc.creatorCarlomagno, Teresa-
Autor(es): dc.creatorCaruso, Icaro Putinhon [UNESP]-
Autor(es): dc.creatorCeylan, Betül-
Autor(es): dc.creatorChaikuad, Apirat-
Autor(es): dc.creatorChu, Feixia-
Autor(es): dc.creatorCole, Laura-
Autor(es): dc.creatorCrosby, Marquise G.-
Autor(es): dc.creatorde Jesus, Vanessa-
Autor(es): dc.creatorDhamotharan, Karthikeyan-
Autor(es): dc.creatorFelli, Isabella C.-
Autor(es): dc.creatorFerner, Jan-
Autor(es): dc.creatorFleischmann, Yanick-
Autor(es): dc.creatorFogeron, Marie-Laure-
Autor(es): dc.creatorFourkiotis, Nikolaos K.-
Autor(es): dc.creatorFuks, Christin-
Autor(es): dc.creatorFürtig, Boris-
Autor(es): dc.creatorGallo, Angelo-
Autor(es): dc.creatorGande, Santosh L.-
Autor(es): dc.creatorGerez, Juan Atilio-
Autor(es): dc.creatorGhosh, Dhiman-
Autor(es): dc.creatorGomes-Neto, Francisco-
Autor(es): dc.creatorGorbatyuk, Oksana-
Autor(es): dc.creatorGuseva, Serafima-
Autor(es): dc.creatorHacker, Carolin-
Autor(es): dc.creatorHäfner, Sabine-
Autor(es): dc.creatorHao, Bing-
Autor(es): dc.creatorHargittay, Bruno-
Autor(es): dc.creatorHenzler-Wildman, K.-
Autor(es): dc.creatorHoch, Jeffrey C.-
Autor(es): dc.creatorHohmann, Katharina F.-
Autor(es): dc.creatorHutchison, Marie T.-
Autor(es): dc.creatorJaudzems, Kristaps-
Autor(es): dc.creatorJović, Katarina-
Autor(es): dc.creatorKaderli, Janina-
Autor(es): dc.creatorKalniņš, Gints-
Autor(es): dc.creatorKaņepe, Iveta-
Autor(es): dc.creatorKirchdoerfer, Robert N.-
Autor(es): dc.creatorKirkpatrick, John-
Autor(es): dc.creatorKnapp, Stefan-
Autor(es): dc.creatorKrishnathas, Robin-
Autor(es): dc.creatorKutz, Felicitas-
Autor(es): dc.creatorzur Lage, Susanne-
Autor(es): dc.creatorLambertz, Roderick-
Autor(es): dc.creatorLang, Andras-
Autor(es): dc.creatorLaurents, Douglas-
Autor(es): dc.creatorLecoq, Lauriane-
Autor(es): dc.creatorLinhard, Verena-
Autor(es): dc.creatorLöhr, Frank-
Autor(es): dc.creatorMalki, Anas-
Autor(es): dc.creatorBessa, Luiza Mamigonian-
Autor(es): dc.creatorMartin, Rachel W.-
Autor(es): dc.creatorMatzel, Tobias-
Autor(es): dc.creatorMaurin, Damien-
Autor(es): dc.creatorMcNutt, Seth W.-
Autor(es): dc.creatorMebus-Antunes, Nathane Cunha-
Autor(es): dc.creatorMeier, Beat H.-
Autor(es): dc.creatorMeiser, Nathalie-
Autor(es): dc.creatorMompeán, Miguel-
Autor(es): dc.creatorMonaca, Elisa-
Autor(es): dc.creatorMontserret, Roland-
Autor(es): dc.creatorMariño Perez, Laura-
Autor(es): dc.creatorMoser, Celine-
Autor(es): dc.creatorMuhle-Goll, Claudia-
Autor(es): dc.creatorNeves-Martins, Thais Cristtina-
Autor(es): dc.creatorNi, Xiamonin-
Autor(es): dc.creatorNorton-Baker, Brenna-
Autor(es): dc.creatorPierattelli, Roberta-
Autor(es): dc.creatorPontoriero, Letizia-
Autor(es): dc.creatorPustovalova, Yulia-
Autor(es): dc.creatorOhlenschläger, Oliver-
Autor(es): dc.creatorOrts, Julien-
Autor(es): dc.creatorDa Poian, Andrea T.-
Autor(es): dc.creatorPyper, Dennis J.-
Autor(es): dc.creatorRichter, Christian-
Autor(es): dc.creatorRiek, Roland-
Autor(es): dc.creatorRienstra, Chad M.-
Autor(es): dc.creatorRobertson, Angus-
Autor(es): dc.creatorPinheiro, Anderson S.-
Autor(es): dc.creatorSabbatella, Raffaele-
Autor(es): dc.creatorSalvi, Nicola-
Autor(es): dc.creatorSaxena, Krishna-
Autor(es): dc.creatorSchulte, Linda-
Autor(es): dc.creatorSchiavina, Marco-
Autor(es): dc.creatorSchwalbe, Harald-
Autor(es): dc.creatorSilber, Mara-
Autor(es): dc.creatorAlmeida, Marcius da Silva-
Autor(es): dc.creatorSprague-Piercy, Marc A.-
Autor(es): dc.creatorSpyroulias, Georgios A.-
Autor(es): dc.creatorSreeramulu, Sridhar-
Autor(es): dc.creatorTants, Jan-Niklas-
Autor(es): dc.creatorTārs, Kaspars-
Autor(es): dc.creatorTorres, Felix-
Autor(es): dc.creatorTöws, Sabrina-
Autor(es): dc.creatorTreviño, Miguel Á.-
Autor(es): dc.creatorTrucks, Sven-
Autor(es): dc.creatorTsika, Aikaterini C.-
Autor(es): dc.creatorVarga, Krisztina-
Autor(es): dc.creatorWang, Ying-
Autor(es): dc.creatorWeber, Marco E.-
Autor(es): dc.creatorWeigand, Julia E.-
Autor(es): dc.creatorWiedemann, Christoph-
Autor(es): dc.creatorWirmer-Bartoschek, Julia-
Autor(es): dc.creatorWirtz Martin, Maria Alexandra-
Autor(es): dc.creatorZehnder, Johannes-
Autor(es): dc.creatorHengesbach, Martin-
Autor(es): dc.creatorSchlundt, Andreas-
Data de aceite: dc.date.accessioned2022-02-22T00:51:11Z-
Data de disponibilização: dc.date.available2022-02-22T00:51:11Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-05-10-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3389/fmolb.2021.653148-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/207711-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/207711-
Descrição: dc.descriptionThe highly infectious disease COVID-19 caused by the Betacoronavirus SARS-CoV-2 poses a severe threat to humanity and demands the redirection of scientific efforts and criteria to organized research projects. The international COVID19-NMR consortium seeks to provide such new approaches by gathering scientific expertise worldwide. In particular, making available viral proteins and RNAs will pave the way to understanding the SARS-CoV-2 molecular components in detail. The research in COVID19-NMR and the resources provided through the consortium are fully disclosed to accelerate access and exploitation. NMR investigations of the viral molecular components are designated to provide the essential basis for further work, including macromolecular interaction studies and high-throughput drug screening. Here, we present the extensive catalog of a holistic SARS-CoV-2 protein preparation approach based on the consortium’s collective efforts. We provide protocols for the large-scale production of more than 80% of all SARS-CoV-2 proteins or essential parts of them. Several of the proteins were produced in more than one laboratory, demonstrating the high interoperability between NMR groups worldwide. For the majority of proteins, we can produce isotope-labeled samples of HSQC-grade. Together with several NMR chemical shift assignments made publicly available on covid19-nmr.com, we here provide highly valuable resources for the production of SARS-CoV-2 proteins in isotope-labeled form.-
Descrição: dc.descriptionInstitute for Organic Chemistry and Chemical Biology Goethe University Frankfurt-
Descrição: dc.descriptionCenter of Biomolecular Magnetic Resonance (BMRZ) Goethe University Frankfurt-
Descrição: dc.descriptionInstitute for Molecular Biosciences Goethe University Frankfurt-
Descrição: dc.descriptionMolecular Microbiology and Structural Biochemistry UMR 5086 CNRS/Lyon University-
Descrição: dc.descriptionInstitute for Biochemistry Goethe University Frankfurt-
Descrição: dc.descriptionSwiss Federal Institute of Technology Laboratory of Physical Chemistry ETH Zurich-
Descrição: dc.descriptionStructural Biology and Biophysics Unit Fondazione Ri.MED-
Descrição: dc.descriptionNational Center of Nuclear Magnetic Resonance (CNRMN CENABIO) Federal University of Rio de Janeiro-
Descrição: dc.descriptionInstitute of Medical Biochemistry Federal University of Rio de Janeiro-
Descrição: dc.descriptionMultidisciplinary Center for Research in Biology (NUMPEX) Caxias Federal University of Rio de Janeiro, Campus Duque de-
Descrição: dc.descriptionInstitute for Molecular Virology University of Wisconsin-Madison-
Descrição: dc.descriptionInstitute of Chemistry Federal University of Rio de Janeiro-
Descrição: dc.descriptionDepartment of Chemistry University of California-
Descrição: dc.descriptionLCP NIDDK-
Descrição: dc.descriptionUniv. Grenoble Alpes CNRS CEA IBS-
Descrição: dc.descriptionLatvian Institute of Organic Synthesis-
Descrição: dc.descriptionBMWZ and Institute of Organic Chemistry Leibniz University Hannover-
Descrição: dc.descriptionGroup of NMR-Based Structural Chemistry Helmholtz Centre for Infection Research-
Descrição: dc.descriptionMultiuser Center for Biomolecular Innovation (CMIB) Department of Physics São Paulo State University (UNESP) São José do Rio Preto-
Descrição: dc.descriptionInstitute of Pharmaceutical Chemistry Goethe University Frankfurt-
Descrição: dc.descriptionStructural Genomics Consortium Buchmann Institute for Molecular Life Sciences-
Descrição: dc.descriptionDepartment of Molecular Cellular and Biomedical Sciences University of New Hampshire-
Descrição: dc.descriptionDepartment of Molecular Biology and Biochemistry University of California-
Descrição: dc.descriptionMagnetic Resonance Centre (CERM) University of Florence-
Descrição: dc.descriptionDepartment of Chemistry “Ugo Schiff” University of Florence-
Descrição: dc.descriptionDepartment of Pharmacy University of Patras-
Descrição: dc.descriptionLaboratory of Toxinology Oswaldo Cruz Foundation (FIOCRUZ)-
Descrição: dc.descriptionDepartment of Molecular Biology and Biophysics UConn Health-
Descrição: dc.descriptionSignals GmbH Co. KG-
Descrição: dc.descriptionLeibniz Institute on Aging—Fritz Lipmann Institute (FLI)-
Descrição: dc.descriptionLatvian Biomedical Research and Study Centre-
Descrição: dc.descriptionRocasolano” Institute for Physical Chemistry (IQFR) Spanish National Research Council (CSIC)-
Descrição: dc.descriptionInstitute of Biophysical Chemistry Goethe University Frankfurt-
Descrição: dc.descriptionKarlsruhe Institute of Technology, IBG-4-
Descrição: dc.descriptionDepartment of Biochemistry and National Magnetic Resonance Facility at Madison University of Wisconsin-Madison-
Descrição: dc.descriptionDepartment of Biology Technical University of Darmstadt-
Descrição: dc.descriptionInstitute of Biochemistry and Biotechnology Charles Tanford Protein Centre Martin Luther University Halle-Wittenberg-
Descrição: dc.descriptionMultiuser Center for Biomolecular Innovation (CMIB) Department of Physics São Paulo State University (UNESP) São José do Rio Preto-
Idioma: dc.languageen-
Relação: dc.relationFrontiers in Molecular Biosciences-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectaccessory proteins-
Palavras-chave: dc.subjectcell-free protein synthesis-
Palavras-chave: dc.subjectCOVID-19-
Palavras-chave: dc.subjectintrinsically disordered region-
Palavras-chave: dc.subjectNMR spectroscopy-
Palavras-chave: dc.subjectnonstructural proteins-
Palavras-chave: dc.subjectSARS-CoV-2-
Palavras-chave: dc.subjectstructural proteins-
Título: dc.titleLarge-Scale Recombinant Production of the SARS-CoV-2 Proteome for High-Throughput and Structural Biology Applications-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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