Suturable elastomeric tubular grafts with patterned porosity for rapid vascularization of 3D constructs

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorEcpm-
Autor(es): dc.contributorUniversidade Federal de São Carlos (UFSCar)-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorMassachusetts Institute of Technology-
Autor(es): dc.contributorHarvard Medical School-
Autor(es): dc.contributorUniversity of California-Los Angeles-
Autor(es): dc.contributorSouth China University of Technology-
Autor(es): dc.creatorBellani, Caroline Faria-
Autor(es): dc.creatorYue, Kan-
Autor(es): dc.creatorFlaig, Florence-
Autor(es): dc.creatorHébraud, Anne-
Autor(es): dc.creatorRay, Pengfei-
Autor(es): dc.creatorAnnabi, Nasim-
Autor(es): dc.creatorSelistre De Araújo, Heloísa Sobreiro-
Autor(es): dc.creatorBranciforti, Márcia Cristina-
Autor(es): dc.creatorMinarelli Gaspar, Ana Maria [UNESP]-
Autor(es): dc.creatorShin, Su Ryon-
Autor(es): dc.creatorKhademhosseini, Ali-
Autor(es): dc.creatorSchlatter, Guy-
Data de aceite: dc.date.accessioned2022-02-22T00:51:07Z-
Data de disponibilização: dc.date.available2022-02-22T00:51:07Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-07-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1088/1758-5090/abdf1d-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/207695-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/207695-
Descrição: dc.descriptionVascularization is considered to be one of the key challenges in engineering functional 3D tissues. Engineering suturable vascular grafts containing pores with diameter of several tens of microns in tissue engineered constructs may provide an instantaneous blood perfusion through the grafts improving cell infiltration and thus, allowing rapid vascularization and vascular branching. The aim of this work was to develop suturable tubular scaffolds to be integrated in biofabricated constructs, enabling the direct connection of the biofabricated construct with the host blood stream, providing an immediate blood flow inside the construct. Here, tubular grafts with customizable shapes (tubes, Y-shape capillaries) and controlled diameter ranging from several hundreds of microns to few mm are fabricated based on poly(glycerol sebacate) (PGS)/poly(vinyl alcohol) (PVA) electrospun scaffolds. Furthermore, a network of pore channels of diameter in the order of 100 μm was machined by laser femtosecond ablation in the tube wall. Both non-machined and laser machined tubular scaffolds elongated more than 100% of their original size have shown suture retention, being 5.85 and 3.96 N mm-2 respectively. To demonstrate the potential of application, the laser machined porous grafts were embedded in gelatin methacryloyl (GelMA) hydrogels, resulting in elastomeric porous tubular graft/GelMA 3D constructs. These constructs were then co-seeded with osteoblast-like cells (MG-63) at the external side of the graft and human umbilical vein endothelial cells inside, forming a bone osteon model. The laser machined pore network allowed an immediate endothelial cell flow towards the osteoblasts enabling the osteoblasts and endothelial cells to interact and form 3D structures. This rapid vascularization approach could be applied, not only for bone tissue regeneration, but also for a variety of tissues and organs.-
Descrição: dc.descriptionBioengineering Department Sao Carlos School of Engineering University of Sao Paulo-
Descrição: dc.descriptionInstitut de Chimie et Procedes Pour l'Energie l'Environnement et la Sante (ICPEES) Umr 7515 CNRS-University of Strasbourg Ecpm-
Descrição: dc.descriptionLaboratory Biochemistry and Molecular Biology Physiological Sciences Department Federal University of Sao Carlos-
Descrição: dc.descriptionMaterials Engineering Department Sao Carlos School of Engineering University of Sao Paulo-
Descrição: dc.descriptionDepartment of Morphology School of Dentistry at Araraquara São Paulo State University (UNESP)-
Descrição: dc.descriptionHarvard-Massachusetts Institute of Technology Division of Health Sciences and Technology Massachusetts Institute of Technology-
Descrição: dc.descriptionDepartment of Medicine Brigham and Women's Hospital Harvard Medical School-
Descrição: dc.descriptionDepartment of Chemical and Biomolecular Engineering University of California-Los Angeles-
Descrição: dc.descriptionCenter for Minimally Invasive Therapeutics (C-MIT) University of California-Los Angeles-
Descrição: dc.descriptionCalifornia NanoSystems Institute University of California-Los Angeles-
Descrição: dc.descriptionSouth China Advanced Institute for Soft Matter Science and Technology South China University of Technology-
Descrição: dc.descriptionDepartment of Morphology School of Dentistry at Araraquara São Paulo State University (UNESP)-
Idioma: dc.languageen-
Relação: dc.relationBiofabrication-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectbio-elastomer-
Palavras-chave: dc.subjectelectrospinning-
Palavras-chave: dc.subjectlaser micromachining-
Palavras-chave: dc.subjectvascularization-
Título: dc.titleSuturable elastomeric tubular grafts with patterned porosity for rapid vascularization of 3D constructs-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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