Intrauterine blood plasma platelet-therapy mitigates persistent breeding-induced endometritis, reduces uterine infections, and improves embryo recovery in Mares

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversity of Illinois Urbana Champaign-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUniversity of Kentucky-
Autor(es): dc.contributorRoss University School of Veterinary Medicine-
Autor(es): dc.contributorUniversity of California-
Autor(es): dc.creatorSegabinazzi, Lorenzo G. T. M. [UNESP]-
Autor(es): dc.creatorCanisso, Igor F.-
Autor(es): dc.creatorPodico, Giorgia-
Autor(es): dc.creatorCunha, Lais L.-
Autor(es): dc.creatorNovello, Guilherme [UNESP]-
Autor(es): dc.creatorRosser, Michael F.-
Autor(es): dc.creatorLoux, Shavahn C.-
Autor(es): dc.creatorLima, Fabio S.-
Autor(es): dc.creatorAlvarenga, Marco A. [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:51:07Z-
Data de disponibilização: dc.date.available2022-02-22T00:51:07Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-05-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/antibiotics10050490-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/207693-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/207693-
Descrição: dc.descriptionMicroorganisms, including pathogenic or opportunistic bacteria and fungi, may gain access to the uterus during breeding, and infectious endometritis plays a major role in equine subfertility. This study aimed to assess the post-breeding inflammatory response, endometrial culture, and embryo recovery of mares susceptible to persistent breeding-induced endometritis (PBIE) treated with plasma-rich (PRP) or-poor (PPP) plasma. Mares (n = 12) susceptible to PBIE had three cycles randomly assigned to receive intrauterine infusions of lactate ringer solution (LRS, control), or autologous PRP or PPP pre-(−48 and −24 h) and post-breeding (6 and 24 h). Mares were bred with fresh semen from one stallion. Intrauterine fluid accumulation (IUF) and endometrial neutrophils were assessed every 24 h up to 96 h post-breeding. Uterine cytokines (Ilβ, IL6, CXCL8, and IL10) were evaluated before (0 h), 6, and 24 h post-breeding, and endometrial culture three and nine days after breed. Embryo flushing was performed 8 days post-ovulation. Data were analyzed with mixed model, Tukey’s post-hoc test, and multivariate regression. PRP treatment reduced endometrial neutrophils, post-breeding IUF, and pro-inflammatory cytokines when compared to control-assigned cycles, but not significantly different than PPP. Controls had a significantly higher percentage of positive bacterial cultures (33%) in comparison to PRP-assigned cycles (0%), whereas cycles treated with PPP were not significantly different from the other groups (25%). The PRP-assigned cycles had significantly greater embryo recovery rates (83%) than the control (33%), though not significantly different than PPP (60%). Plasma infusion reduced the duration and intensity of the post-breeding inflammatory response and improved embryo recovery in mares susceptible to PBIE. Platelets incrementally downregulate PBIE and appear to have a dose-dependent antimicrobial property.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionU.S. Department of Agriculture-
Descrição: dc.descriptionDepartment of Veterinary Clinical Medicine College of Veterinary Medicine University of Illinois Urbana Champaign, 1008 W Hazelwood Drive-
Descrição: dc.descriptionDepartment of Veterinary Surgery and Animal Reproduction School of Veterinary Medicine and Animal Science São Paulo State University (UNESP)-
Descrição: dc.descriptionVeterinary Diagnostic Laboratory College of Veterinary Medicine University of Illinois Urbana Champaign-
Descrição: dc.descriptionMaxwell H. Gluck Equine Research Center University of Kentucky-
Descrição: dc.descriptionRoss University School of Veterinary Medicine, PO Box 334-
Descrição: dc.descriptionDepartment of Population Health and Reproduction School of Veterinary Medicine University of California-
Descrição: dc.descriptionDepartment of Veterinary Surgery and Animal Reproduction School of Veterinary Medicine and Animal Science São Paulo State University (UNESP)-
Descrição: dc.descriptionFAPESP: 2018/02856-3-
Descrição: dc.descriptionU.S. Department of Agriculture: ILLU-888-912-
Idioma: dc.languageen-
Relação: dc.relationAntibiotics-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectEndometrium-
Palavras-chave: dc.subjectEquine-
Palavras-chave: dc.subjectImmunomodulation-
Palavras-chave: dc.subjectPRP-
Palavras-chave: dc.subjectUterine inflammation-
Título: dc.titleIntrauterine blood plasma platelet-therapy mitigates persistent breeding-induced endometritis, reduces uterine infections, and improves embryo recovery in Mares-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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