Modulatory effects of acidic ph and membrane potential on the adsorption of ph-sensitive peptides to anionic lipid membrane

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.creatorAlvares, Dayane Dos Santos [UNESP]-
Autor(es): dc.creatorMartins, Ingrid Bernardes Santana [UNESP]-
Autor(es): dc.creatorViegas, Taisa Giordano [UNESP]-
Autor(es): dc.creatorPalma, Mario Sergio [UNESP]-
Autor(es): dc.creatorde Araujo, Alexandre Suman [UNESP]-
Autor(es): dc.creatorde Carvalho, Sidney Jurado [UNESP]-
Autor(es): dc.creatorNeto, João Ruggiero [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:51:06Z-
Data de disponibilização: dc.date.available2022-02-22T00:51:06Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-05-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/membranes11050307-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/207689-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/207689-
Descrição: dc.descriptionAnionic lipid membrane electrostatic potential and solution pH can influence cationic peptide adsorption to these bilayers, especially those containing simultaneously acid and basic residues. Here, we investigate the effects of the pH solution on MP1 (IDWKKLLDAAKQIL-NH2) adsorption to anionic (7POPC:3POPG) lipid vesicles in comparison to its analog H-MP1, with histidines substituting lysines. We used the association of adsorption isotherms and constant pH molecular dynamic simulations (CpHMD) to explore the effects of membrane potential and pH on peptides’ adsorption on this lipid membrane. We analyzed the fluorescence and zeta potential adsorption isotherms using the Gouy–Chapman theory. In CpHMD simulations for the peptides in solution and adsorbed on the lipid bilayer, we used the conformations obtained by conventional MD simulations at a µs timescale. Non-equilibrium Monte Carlo simulations provided the protonation states of acidic and basic residues. CpHMD showed average pKa shifts of two to three units, resulting in a higher net charge for the analog than for MP1, strongly modulating the peptide adsorption. The fractions of the protonation of acidic and basic residues and the peptides’ net charges obtained from the analysis of the adsorption isotherms were in reasonable agreement with those from CpHMD. MP1 adsorption was almost insensitive to solution pH. H-MP1 was much more sensitive to partitioning, at acidic pH, with an affinity ten times higher than in neutral ones.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionIBILCE Department of Physics UNESP—São Paulo State University-
Descrição: dc.descriptionInstitute of Biosciences Department of Basic and Applied Biology UNESP—São Paulo State University-
Descrição: dc.descriptionIBILCE Department of Physics UNESP—São Paulo State University-
Descrição: dc.descriptionInstitute of Biosciences Department of Basic and Applied Biology UNESP—São Paulo State University-
Descrição: dc.descriptionFAPESP: 2010/18169-3-
Descrição: dc.descriptionFAPESP: 2015/25619-9-
Descrição: dc.descriptionFAPESP: 2016/16212-5-
Descrição: dc.descriptionFAPESP: 2018/01841-2-
Idioma: dc.languageen-
Relação: dc.relationMembranes-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectCpHMD-
Palavras-chave: dc.subjectFluorescence spectroscopy-
Palavras-chave: dc.subjectMembrane potential-
Palavras-chave: dc.subjectPH-responsive peptides-
Palavras-chave: dc.subjectZeta potential-
Título: dc.titleModulatory effects of acidic ph and membrane potential on the adsorption of ph-sensitive peptides to anionic lipid membrane-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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