Insights about the epidemiology of Salmonella Typhimurium isolates from different sources in Brazil using comparative genomics

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Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorInstituto Adolfo Lutz de Ribeirão Preto-
Autor(es): dc.contributorFundação Oswaldo Cruz – FIOCRUZ-
Autor(es): dc.contributorEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)-
Autor(es): dc.contributorUniversidade Federal do Triângulo Mineiro – UFTM-
Autor(es): dc.contributorFood and Drug Administration-FDA-
Autor(es): dc.creatorSeribelli, Amanda Ap.-
Autor(es): dc.creatorda Silva, Patrick [UNESP]-
Autor(es): dc.creatorda Cruz, Marcelo Ferreira-
Autor(es): dc.creatorde Almeida, Fernanda-
Autor(es): dc.creatorFrazão, Miliane R.-
Autor(es): dc.creatorMedeiros, Marta I. C.-
Autor(es): dc.creatorRodrigues, Dália dos P.-
Autor(es): dc.creatorKich, Jalusa D.-
Autor(es): dc.creatorde Jesus Benevides, Leandro-
Autor(es): dc.creatorSoares, Siomar de C.-
Autor(es): dc.creatorAllard, Marc W.-
Autor(es): dc.creatorFalcão, Juliana Pfrimer.-
Data de aceite: dc.date.accessioned2022-02-22T00:51:01Z-
Data de disponibilização: dc.date.available2022-02-22T00:51:01Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-11-30-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1186/s13099-021-00423-7-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/207667-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/207667-
Descrição: dc.descriptionBackground: Salmonella enterica subsp. enterica serovar Typhimurium (S. Typhimurium) is an important zoonotic agent worldwide. The aim of this work was to compare genetically 117 S. Typhimurium isolated from different sources over 30 years in Brazil using different genomics strategies. Results: The majority of the 117 S. Typhimurium strains studied were grouped into a single cluster (≅ 90%) by the core genome multilocus sequence typing and (≅ 77%) by single copy marker genes. The phylogenetic analysis based on single nucleotide polymorphism (SNP) grouped most strains from humans into a single cluster (≅ 93%), while the strains isolated from food and swine were alocated into three clusters. The different orthologous protein clusters found for some S. Typhimurium isolated from humans and food are involved in metabolic and regulatory processes. For 26 isolates from swine the sequence types (ST) 19 and ST1921 were the most prevalent ones, and the ST14, ST64, ST516 and ST639 were also detected. Previous results typed the 91 S. Typhimurium isolates from humans and foods as ST19, ST313, ST1921, ST3343 and ST1649. The main prophages detected were: Gifsy-2 in 79 (67.5%) and Gifsy-1 in 63 (54%) strains. All of the S. Typhimurium isolates contained the acrA, acrB, macA, macB, mdtK, emrA, emrB, emrR and tolC efflux pump genes. Conclusions: The phylogenetic trees grouped the majority of the S. Typhimurium isolates from humans into a single cluster suggesting that there is one prevalent subtype in Brazil. Regarding strains isolated from food and swine, the SNPs’ results suggested the circulation of more than one subtype over 30 years in this country. The orthologous protein clusters analysis revealed unique genes in the strains studied mainly related to bacterial metabolism. S. Typhimurium strains from swine showed greater diversity of STs and prophages in comparison to strains isolated from humans and foods. The pathogenic potential of S. Typhimurium strains was corroborated by the presence of exclusive prophages of this serovar involved in its virulence. The high number of resistance genes related to efflux pumps is worrying and may lead to therapeutic failures when clinical treatment is needed.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionDepartamento de Análises Clínicas Toxicológicas e Bromatológicas Faculdade de Ciências Farmacêuticas de Ribeirão Preto Universidade de São Paulo - USP-
Descrição: dc.descriptionFaculdade de Ciências Farmacêuticas de Araraquara UNESP – Departamento de Ciências Biológicas, Rodovia Araraquara-Jaú Km 1-
Descrição: dc.descriptionInstituto Adolfo Lutz de Ribeirão Preto-
Descrição: dc.descriptionFundação Oswaldo Cruz – FIOCRUZ-
Descrição: dc.descriptionEmpresa Brasileira de Pesquisa Agropecuária – Suínos e Aves – EMBRAPA-
Descrição: dc.descriptionUniversidade Federal do Triângulo Mineiro – UFTM-
Descrição: dc.descriptionFood and Drug Administration-FDA-
Descrição: dc.descriptionFaculdade de Ciências Farmacêuticas de Araraquara UNESP – Departamento de Ciências Biológicas, Rodovia Araraquara-Jaú Km 1-
Descrição: dc.descriptionFAPESP: 2016/24716-3-
Descrição: dc.descriptionFAPESP: 2017/06633-6-
Descrição: dc.descriptionFAPESP: 2019/19338-8-
Descrição: dc.descriptionCNPq: 304399/2018-3-
Descrição: dc.descriptionCAPES: Finance code 001-
Idioma: dc.languageen-
Relação: dc.relationGut Pathogens-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectEfflux pumps-
Palavras-chave: dc.subjectPhylogenetic trees-
Palavras-chave: dc.subjectProphages-
Palavras-chave: dc.subjectProtein orthologous clusters-
Palavras-chave: dc.subjectSalmonella Typhimurium-
Título: dc.titleInsights about the epidemiology of Salmonella Typhimurium isolates from different sources in Brazil using comparative genomics-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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