Atenção: Todas as denúncias são sigilosas e sua identidade será preservada.
Os campos nome e e-mail são de preenchimento opcional
Metadados | Descrição | Idioma |
---|---|---|
Autor(es): dc.contributor | Universidade Estadual de Londrina (UEL) | - |
Autor(es): dc.contributor | Anhanguera-Uniderp University | - |
Autor(es): dc.contributor | Universidade Estadual Paulista (Unesp) | - |
Autor(es): dc.contributor | Instituto de Investigaciones Biológicas Clemente Estable | - |
Autor(es): dc.creator | Serpeloni, Juliana Mara | - |
Autor(es): dc.creator | Specian, Ana Flavia Leal | - |
Autor(es): dc.creator | Ribeiro, Diego Luis | - |
Autor(es): dc.creator | Tuttis, Katiuska | - |
Autor(es): dc.creator | Heredia-Vieira, Silvia Cristina | - |
Autor(es): dc.creator | Vilegas, Wagner [UNESP] | - |
Autor(es): dc.creator | Martínez-López, Wilner | - |
Autor(es): dc.creator | Varanda, Eliana Aparecida [UNESP] | - |
Autor(es): dc.creator | de Syllos Cólus, Ilce Mara | - |
Data de aceite: dc.date.accessioned | 2022-02-22T00:50:56Z | - |
Data de disponibilização: dc.date.available | 2022-02-22T00:50:56Z | - |
Data de envio: dc.date.issued | 2021-06-25 | - |
Data de envio: dc.date.issued | 2021-06-25 | - |
Data de envio: dc.date.issued | 2020-12-31 | - |
Fonte completa do material: dc.identifier | http://dx.doi.org/10.1002/tox.23151 | - |
Fonte completa do material: dc.identifier | http://hdl.handle.net/11449/207640 | - |
Fonte: dc.identifier.uri | http://educapes.capes.gov.br/handle/11449/207640 | - |
Descrição: dc.description | Gastric cancer is the fifth most common malignancy worldwide. Serjania marginata Casar. (SM) displays anti-inflammatory properties and has been used to treat gastrointestinal disorders. In the current study, we examined whether the hydroethanolic extract of SM leaves exerted cytotoxic, mutagenic, and protective effects in non-tumor gastric epithelium cells (MNP01) and gastric adenocarcinoma cells (ACP02) in vitro and analyzed whether its action was selective. Initially, cell viability (MTT assay), cell cycle kinetics (flow cytometry), and cell proliferation (total protein content) were analyzed. In addition, genomic instability (cytokinesis-block micronucleus cytome assay), anti/pro-oxidant status (CM-H2DCFDA probe), and transcriptional expression (RT-qPCR) of genes related to cell cycle, cell death, and antioxidant defense were also evaluated. The SM extract was cytotoxic toward MNP01 and ACP02 cells at concentrations greater than 300 and 100 μg·ml−1, respectively, and decreased protein content only toward ACP02 cells at 200 μg ml−1. In ACP02 cells, the SM extract at 100 μg·ml−1 associated with doxorubicin (DXR; 0.2 μg ml−1) clearly promoted cell cycle arrest at the G2/M phase. The extract alone was not mutagenic to either cell type and reversed DXR-induced DNA damage and H2O2-induced oxidative stress in MNP01 cells. The gene expression experiments showed that SM hydroethanolic extract exerts an antioxidant response via NFE2L2 activation in non-tumor gastric cells, and cell cycle arrest (G2/M) in ACP02 gastric cancer cells via the TP53 pathway. The selective action of SM indicates that it is a promising therapeutic agent to treat gastric diseases and merits further studies. | - |
Descrição: dc.description | Department of General Biology Center of Biological Sciences State University of Londrina (UEL) | - |
Descrição: dc.description | Natural Products Laboratory Anhanguera-Uniderp University | - |
Descrição: dc.description | Experimental Campus of São Vicente São Paulo State University (UNESP) | - |
Descrição: dc.description | Epigenetics and Genomic Instability Laboratory Instituto de Investigaciones Biológicas Clemente Estable | - |
Descrição: dc.description | Department of Biological Sciences School of Pharmaceutical Sciences São Paulo State University (UNESP) | - |
Descrição: dc.description | Experimental Campus of São Vicente São Paulo State University (UNESP) | - |
Descrição: dc.description | Department of Biological Sciences School of Pharmaceutical Sciences São Paulo State University (UNESP) | - |
Idioma: dc.language | en | - |
Relação: dc.relation | Environmental Toxicology | - |
???dc.source???: dc.source | Scopus | - |
Palavras-chave: dc.subject | antimutagenic | - |
Palavras-chave: dc.subject | antioxidant | - |
Palavras-chave: dc.subject | cipó-timbó | - |
Palavras-chave: dc.subject | cytotoxicity | - |
Palavras-chave: dc.subject | Sapindaceae | - |
Título: dc.title | Selective anticancer effects of Serjania marginata Casar. extract in gastric cells are mediated by antioxidant response | - |
Tipo de arquivo: dc.type | livro digital | - |
Aparece nas coleções: | Repositório Institucional - Unesp |
O Portal eduCAPES é oferecido ao usuário, condicionado à aceitação dos termos, condições e avisos contidos aqui e sem modificações. A CAPES poderá modificar o conteúdo ou formato deste site ou acabar com a sua operação ou suas ferramentas a seu critério único e sem aviso prévio. Ao acessar este portal, você, usuário pessoa física ou jurídica, se declara compreender e aceitar as condições aqui estabelecidas, da seguinte forma: