Allogenic mesenchymal stem cell-conditioned medium does not affect sperm parameters and mitigates early endometrial inflammatory responses in mares

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.creatorTongu, Eriky Akio de Oliveira [UNESP]-
Autor(es): dc.creatorSegabinazzi, Lorenzo G.T.M. [UNESP]-
Autor(es): dc.creatorAlvarenga, Marina Landim [UNESP]-
Autor(es): dc.creatorMonteiro, Aldine [UNESP]-
Autor(es): dc.creatorPapa, Frederico Ozanam [UNESP]-
Autor(es): dc.creatorAlvarenga, Marco Antonio [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:50:54Z-
Data de disponibilização: dc.date.available2022-02-22T00:50:54Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-07-15-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.theriogenology.2021.03.019-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/207628-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/207628-
Descrição: dc.descriptionThis study aimed to evaluate the effects of mesenchymal stem cell-conditioned medium (MSC-CM) on sperm parameters, intrauterine polymorphonuclear neutrophils (PMN), intrauterine fluid accumulation (IUF), and fertility in mares. In experiment 1, two ejaculates from ten stallions were extended to 50 million sperm/mL using a milk-based extender. Thereafter, 20 mL of extended semen was added of MSC-CM as follows: 0, 5, 10, 15, and 20 mL. Sperm kinetics and plasma membrane integrity were evaluated immediately after dilution (T0) and 2 h post-incubation at 37 °C (T2). In experiment 2, mares characterized as resistant (n = 13) or susceptible (n = 7) to endometritis were inseminated with fresh semen 24 h post-induction of ovulation in two (Control and CM-1) and three (Control, CM-1, and CM-2) cycles in a crossover, as follows: control, no pharmacological interference; CM-1, supplementation of semen insemination dose at 3:4 (v:v, MSC-CM:semen); CM-2, 30 mL of MSC-CM was infused into the uterus 24 h before insemination. Endometrial cytology and uterine fluid were collected 6 and 24 h after insemination to evaluate the number of PMNs and concentrations of interleukins IL6, IL10, and TNFα. IUF was determined by ultrasonography 24 and 48 h after insemination. Pregnancy status was diagnosed 14 days after ovulation. The addition of MSC-CM to semen did not influence sperm parameters at T0 and T2 (P > 0.05) and reduced (CM-1; P < 0.05) the number of PMNs at 6 h post-insemination in resistant mares. In susceptible mares, PMNs at 6 and 24 h post-insemination, as well as IUF were reduced (P < 0.05) in both treated cycles (CM-1 and CM-2). In addition, MSC-CM downregulated IL6 and upregulated IL10 concentrations in the uterus of susceptible mares after insemination. There were no differences in fertility rates among groups both in resistant (Control, 77%, 10/13; CM-1, 62%, 8/13) and susceptible mares (Control, 42.8%, 3/7; CM-1, 57.1%, 4/7; CM-2, 85.7%. 6/7). In conclusion, MSC-CM did not affect sperm parameters when mixed with diluted semen, and reduced post-insemination inflammatory responses in mares.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionDepartment of Veterinary Surgery and Animal Reproduction Faculty of Veterinary Medicine and Animal Science FMVZ Universidade Estadual Paulista “Júlio de Mesquita Filho” UNESP-
Descrição: dc.descriptionDepartment of Veterinary Surgery and Animal Reproduction Faculty of Veterinary Medicine and Animal Science FMVZ Universidade Estadual Paulista “Júlio de Mesquita Filho” UNESP-
Descrição: dc.descriptionFAPESP: 2018/02856-3-
Formato: dc.format1-8-
Idioma: dc.languageen-
Relação: dc.relationTheriogenology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectCell-therapy-
Palavras-chave: dc.subjectEndometritis-
Palavras-chave: dc.subjectEquine-
Palavras-chave: dc.subjectFertility-
Palavras-chave: dc.subjectUterus-
Título: dc.titleAllogenic mesenchymal stem cell-conditioned medium does not affect sperm parameters and mitigates early endometrial inflammatory responses in mares-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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