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Metadados | Descrição | Idioma |
---|---|---|
Autor(es): dc.contributor | Universidade Estadual Paulista (Unesp) | - |
Autor(es): dc.creator | Tongu, Eriky Akio de Oliveira [UNESP] | - |
Autor(es): dc.creator | Segabinazzi, Lorenzo G.T.M. [UNESP] | - |
Autor(es): dc.creator | Alvarenga, Marina Landim [UNESP] | - |
Autor(es): dc.creator | Monteiro, Aldine [UNESP] | - |
Autor(es): dc.creator | Papa, Frederico Ozanam [UNESP] | - |
Autor(es): dc.creator | Alvarenga, Marco Antonio [UNESP] | - |
Data de aceite: dc.date.accessioned | 2022-02-22T00:50:54Z | - |
Data de disponibilização: dc.date.available | 2022-02-22T00:50:54Z | - |
Data de envio: dc.date.issued | 2021-06-25 | - |
Data de envio: dc.date.issued | 2021-06-25 | - |
Data de envio: dc.date.issued | 2021-07-15 | - |
Fonte completa do material: dc.identifier | http://dx.doi.org/10.1016/j.theriogenology.2021.03.019 | - |
Fonte completa do material: dc.identifier | http://hdl.handle.net/11449/207628 | - |
Fonte: dc.identifier.uri | http://educapes.capes.gov.br/handle/11449/207628 | - |
Descrição: dc.description | This study aimed to evaluate the effects of mesenchymal stem cell-conditioned medium (MSC-CM) on sperm parameters, intrauterine polymorphonuclear neutrophils (PMN), intrauterine fluid accumulation (IUF), and fertility in mares. In experiment 1, two ejaculates from ten stallions were extended to 50 million sperm/mL using a milk-based extender. Thereafter, 20 mL of extended semen was added of MSC-CM as follows: 0, 5, 10, 15, and 20 mL. Sperm kinetics and plasma membrane integrity were evaluated immediately after dilution (T0) and 2 h post-incubation at 37 °C (T2). In experiment 2, mares characterized as resistant (n = 13) or susceptible (n = 7) to endometritis were inseminated with fresh semen 24 h post-induction of ovulation in two (Control and CM-1) and three (Control, CM-1, and CM-2) cycles in a crossover, as follows: control, no pharmacological interference; CM-1, supplementation of semen insemination dose at 3:4 (v:v, MSC-CM:semen); CM-2, 30 mL of MSC-CM was infused into the uterus 24 h before insemination. Endometrial cytology and uterine fluid were collected 6 and 24 h after insemination to evaluate the number of PMNs and concentrations of interleukins IL6, IL10, and TNFα. IUF was determined by ultrasonography 24 and 48 h after insemination. Pregnancy status was diagnosed 14 days after ovulation. The addition of MSC-CM to semen did not influence sperm parameters at T0 and T2 (P > 0.05) and reduced (CM-1; P < 0.05) the number of PMNs at 6 h post-insemination in resistant mares. In susceptible mares, PMNs at 6 and 24 h post-insemination, as well as IUF were reduced (P < 0.05) in both treated cycles (CM-1 and CM-2). In addition, MSC-CM downregulated IL6 and upregulated IL10 concentrations in the uterus of susceptible mares after insemination. There were no differences in fertility rates among groups both in resistant (Control, 77%, 10/13; CM-1, 62%, 8/13) and susceptible mares (Control, 42.8%, 3/7; CM-1, 57.1%, 4/7; CM-2, 85.7%. 6/7). In conclusion, MSC-CM did not affect sperm parameters when mixed with diluted semen, and reduced post-insemination inflammatory responses in mares. | - |
Descrição: dc.description | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) | - |
Descrição: dc.description | Department of Veterinary Surgery and Animal Reproduction Faculty of Veterinary Medicine and Animal Science FMVZ Universidade Estadual Paulista “Júlio de Mesquita Filho” UNESP | - |
Descrição: dc.description | Department of Veterinary Surgery and Animal Reproduction Faculty of Veterinary Medicine and Animal Science FMVZ Universidade Estadual Paulista “Júlio de Mesquita Filho” UNESP | - |
Descrição: dc.description | FAPESP: 2018/02856-3 | - |
Formato: dc.format | 1-8 | - |
Idioma: dc.language | en | - |
Relação: dc.relation | Theriogenology | - |
???dc.source???: dc.source | Scopus | - |
Palavras-chave: dc.subject | Cell-therapy | - |
Palavras-chave: dc.subject | Endometritis | - |
Palavras-chave: dc.subject | Equine | - |
Palavras-chave: dc.subject | Fertility | - |
Palavras-chave: dc.subject | Uterus | - |
Título: dc.title | Allogenic mesenchymal stem cell-conditioned medium does not affect sperm parameters and mitigates early endometrial inflammatory responses in mares | - |
Tipo de arquivo: dc.type | livro digital | - |
Aparece nas coleções: | Repositório Institucional - Unesp |
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