Identification of Fusobacterium nucleatum in primary and secondary endodontic infections and its association with clinical features by using two different methods

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Autor(es): dc.contributorUniversidade Estadual de Campinas (UNICAMP)-
Autor(es): dc.contributorUniversity Center Lusíada – UNILUS-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversidade Federal de Pernambuco (UFPE)-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.creatorGomes, Brenda P. F. A.-
Autor(es): dc.creatorBronzato, Juliana D.-
Autor(es): dc.creatorAlmeida-Gomes, Rebecca F.-
Autor(es): dc.creatorPinheiro, Ericka T.-
Autor(es): dc.creatorSousa, Ezilmara L. R.-
Autor(es): dc.creatorJacinto, Rogério C. [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:50:52Z-
Data de disponibilização: dc.date.available2022-02-22T00:50:52Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2020-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1007/s00784-021-03923-7-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/207620-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/207620-
Descrição: dc.descriptionObjective: Fusobacterium nucleatum is an important oral pathogen involved in endodontic infections. This study aimed to assess the frequency of Fusobacterium nucleatum in primary and secondary endodontic infections and its associations with the clinical features in a Brazilian population by using both culture and nested PCR methods. Methods: A total of 100 microbial samples from patients with primary (n=50) and secondary endodontic infections (n=50) were analyzed by using culture and nested PCR methods. Strict anaerobic techniques were used for culture and identification of F. nucleatum. The DNA extracted from the samples was analyzed for the presence of target species by using species-specific primers. Results: Culture and nested PCR methods detected F. nucleatum, respectively, in 11/100 and 82/100 root canals. F. nucleatum was isolated by culture from 10/50 (20%) root canals with primary infections and from 1/50 (2%) root canal with secondary/persistent infections. Nested PCR detected F. nucleatum in 42/50 (84%) root canals with primary infections and in 40/50 (80%) root canals with secondary/persistent endodontic infections. F. nucleatum was associated with spontaneous pain, tenderness to percussion, pain on palpation, swelling, tooth mobility, wet root canals, hemorrhagic exudate, tooth decay, inadequate restoration, and poor endodontic filling. Conclusion: F. nucleatum was found in more cases of primary endodontic infections than in cases of secondary/persistent ones. A higher prevalence of F. nucleatum was detected by using the nested PCR method than by using culture. The presence of F. nucleatum in the root canals was associated with several clinical features. Clinical relevance: The high prevalence of F. nucleatum in the root canals detected by molecular methods, and its association with several clinical features reveals the importance of these species in the development of apical pathologies and reinforces the need of an endodontic treatment directed to bacterial elimination.-
Descrição: dc.descriptionDepartment of Restorative Dentistry Division of Endodontics Piracicaba Dental School State University of Campinas – UNICAMP, Av. Limeira 901. Bairro Areao-
Descrição: dc.descriptionFaculty of Medical Sciences University Center Lusíada – UNILUS-
Descrição: dc.descriptionDepartment of Operative Dentistry Division of Endodontics The University of São Paulo School of Dentistry – FOUSP-
Descrição: dc.descriptionDepartment of Semiology and Clinic Division of Endodontics School of Dentistry Federal University of Pelotas – UFPEL-
Descrição: dc.descriptionDepartment of Preventive and Restorative Dentistry School of Dentistry Araçatuba São Paulo State University – UNESP-
Descrição: dc.descriptionDepartment of Preventive and Restorative Dentistry School of Dentistry Araçatuba São Paulo State University – UNESP-
Idioma: dc.languageen-
Relação: dc.relationClinical Oral Investigations-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectCulture-
Palavras-chave: dc.subjectEndodontics-
Palavras-chave: dc.subjectFusobacterium nucleatum-
Palavras-chave: dc.subjectPCR-
Título: dc.titleIdentification of Fusobacterium nucleatum in primary and secondary endodontic infections and its association with clinical features by using two different methods-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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