Transcriptome of Two Canine Prostate Cancer Cells Treated With Toceranib Phosphate Reveals Distinct Antitumor Profiles Associated With the PDGFR Pathway

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorPaulista University—UNIP-
Autor(es): dc.creatorKobayashi, Priscila E. [UNESP]-
Autor(es): dc.creatorLainetti, Patrícia F. [UNESP]-
Autor(es): dc.creatorLeis-Filho, Antonio F. [UNESP]-
Autor(es): dc.creatorDelella, Flávia K. [UNESP]-
Autor(es): dc.creatorCarvalho, Marcio [UNESP]-
Autor(es): dc.creatorCury, Sarah Santiloni [UNESP]-
Autor(es): dc.creatorCarvalho, Robson Francisco [UNESP]-
Autor(es): dc.creatorFonseca-Alves, Carlos E. [UNESP]-
Autor(es): dc.creatorLaufer-Amorim, Renée [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:50:40Z-
Data de disponibilização: dc.date.available2022-02-22T00:50:40Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2020-11-25-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3389/fvets.2020.561212-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/207577-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/207577-
Descrição: dc.descriptionCanine prostate cancer (PC) presents a poor antitumor response, usually late diagnosis and prognosis. Toceranib phosphate (TP) is a nonspecific inhibitor of receptor tyrosine kinases (RTKs), including vascular endothelial growth factor receptor (VEGFR), platelet-derived growth factor receptor (PDGFR), and c-KIT. This study aimed to evaluate VEGFR2, PDGFR-β, and c-KIT protein expression in two established canine PC cell lines (PC1 and PC2) and the transcriptome profile of the cells after treatment with TP. Immunofluorescence (IF) analysis revealed VEGFR2 and PDGFR-β protein expression and the absence of c-KIT protein expression in both cell lines. After TP treatment, only the viability of PC1 cells decreased in a dose-dependent manner. Transcriptome and enrichment analyses of treated PC1 cells revealed 181 upregulated genes, which were related to decreased angiogenesis and cell proliferation. In addition, we found upregulated PDGFR-A, PDGFR-β, and PDGF-D expression in PC1 cells, and the upregulation of PDGFR-β was also observed in treated PC1 cells by qPCR. PC2 cells had fewer protein-protein interactions (PPIs), with 18 upregulated and 22 downregulated genes; the upregulated genes were involved in the regulation of parallel pathways and mechanisms related to proliferation, which could be associated with the resistance observed after treatment. The canine PC1 cell line but not the PC2 cell line showed decreased viability after treatment with TP, although both cell lines expressed PDGFR and VEGFR receptors. Further studies could explain the mechanism of resistance in PC2 cells and provide a basis for personalized treatment for dogs with PC.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionDepartment of Veterinary Clinic School of Veterinary Medicine and Animal Science São Paulo State University—UNESP-
Descrição: dc.descriptionDepartment of Veterinary Surgery and Anesthesiology School of Veterinary Medicine and Animal Science São Paulo State University—UNESP-
Descrição: dc.descriptionDepartment of Morphology Institute of Biosciences São Paulo State University—UNESP-
Descrição: dc.descriptionInstitute of Health Sciences Paulista University—UNIP-
Descrição: dc.descriptionDepartment of Veterinary Clinic School of Veterinary Medicine and Animal Science São Paulo State University—UNESP-
Descrição: dc.descriptionDepartment of Veterinary Surgery and Anesthesiology School of Veterinary Medicine and Animal Science São Paulo State University—UNESP-
Descrição: dc.descriptionDepartment of Morphology Institute of Biosciences São Paulo State University—UNESP-
Descrição: dc.descriptionCAPES: 88882.180541/2018-01-
Idioma: dc.languageen-
Relação: dc.relationFrontiers in Veterinary Science-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectanimal model-
Palavras-chave: dc.subjectantitumor response-
Palavras-chave: dc.subjectdog-
Palavras-chave: dc.subjectmicroarray-
Palavras-chave: dc.subjectprostate-
Título: dc.titleTranscriptome of Two Canine Prostate Cancer Cells Treated With Toceranib Phosphate Reveals Distinct Antitumor Profiles Associated With the PDGFR Pathway-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

Não existem arquivos associados a este item.