Cystatin-like protein of sweet orange (CsinCPI-2) modulates pre-osteoblast differentiation via β-Catenin involvement

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Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUniversidade Federal de São Carlos (UFSCar)-
Autor(es): dc.creatorda Costa Fernandes, Célio [UNESP]-
Autor(es): dc.creatorRodríguez, Victor Manuel Ochoa [UNESP]-
Autor(es): dc.creatorSoares-Costa, Andrea-
Autor(es): dc.creatorCirelli, Joni Augusto [UNESP]-
Autor(es): dc.creatorJustino, Daniela Morilha Neo-
Autor(es): dc.creatorRoma, Bárbara [UNESP]-
Autor(es): dc.creatorZambuzzi, Willian Fernando [UNESP]-
Autor(es): dc.creatorFaria, Gisele [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:50:30Z-
Data de disponibilização: dc.date.available2022-02-22T00:50:30Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-04-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1007/s10856-021-06504-y-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/207525-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/207525-
Descrição: dc.descriptionPhytocystatins are endogenous cysteine-protease inhibitors present in plants. They are involved in initial germination rates and in plant defense mechanisms against phytopathogens. Recently, a new phytocystatin derived from sweet orange, CsinCPI-2, has been shown to inhibit the enzymatic activity of human cathepsins, presenting anti-inflammatory potential and pro-osteogenic effect in human dental pulp cells. The osteogenic potential of the CsinCPI-2 protein represents a new insight into plants cysteine proteases inhibitors and this effect needs to be better addressed. The aim of this study was to investigate the performance of pre-osteoblasts in response to CsinCPI-2, mainly focusing on cell adhesion, proliferation and differentiation mechanisms. Together our data show that in the first hours of treatment, protein in CsinCPI-2 promotes an increase in the expression of adhesion markers, which decrease after 24 h, leading to the activation of Kinase-dependent cyclines (CDKs) modulating the transition from G1 to S phases cell cycle. In addition, we saw that the increase in ERK may be associated with activation of the differentiation profile, also observed with an increase in the B-Catenin pathway and an increase in the expression of Runx2 in the group that received the treatment with CsinCPI-2. [Figure not available: see fulltext.].-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionDepartment of Chemistry and Biochemistry Laboratory of Bioassays and Cell Dynamics Institute of Biosciences Sao Paulo State University – UNESP-
Descrição: dc.descriptionDepartment of Restorative Dentistry School of Dentistry at Araraquara Sao Paulo State University – UNESP-
Descrição: dc.descriptionDepartment of Genetic and Evolution Federal University of Sao Carlos-
Descrição: dc.descriptionDepartment of Diagnosis and Surgery School of Dentistry at Araraquara Sao Paulo State University–UNESP-
Descrição: dc.descriptionDepartment of Chemistry and Biochemistry Laboratory of Bioassays and Cell Dynamics Institute of Biosciences Sao Paulo State University – UNESP-
Descrição: dc.descriptionDepartment of Restorative Dentistry School of Dentistry at Araraquara Sao Paulo State University – UNESP-
Descrição: dc.descriptionDepartment of Diagnosis and Surgery School of Dentistry at Araraquara Sao Paulo State University–UNESP-
Descrição: dc.descriptionFAPESP: 2012/24278-5-
Idioma: dc.languageen-
Relação: dc.relationJournal of Materials Science: Materials in Medicine-
???dc.source???: dc.sourceScopus-
Título: dc.titleCystatin-like protein of sweet orange (CsinCPI-2) modulates pre-osteoblast differentiation via β-Catenin involvement-
Tipo de arquivo: dc.typelivro digital-
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