Ascorbic acid supplementation improves skeletal muscle growth in pacu (Piaractus mesopotamicus) juveniles: In vivo and in vitro studies

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Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUniversidade Federal de Goiás (UFG)-
Autor(es): dc.contributorUniversity of Western São Paulo-
Autor(es): dc.creatorZanella, Bruna Tereza Thomazini [UNESP]-
Autor(es): dc.creatorMagiore, Isabele Cristina [UNESP]-
Autor(es): dc.creatorDuran, Bruno Oliveira Silva-
Autor(es): dc.creatorPereira, Guilherme Gutierrez [UNESP]-
Autor(es): dc.creatorVicente, Igor Simões Tiagua [UNESP]-
Autor(es): dc.creatorCarvalho, Pedro Luiz Pucci Figueiredo [UNESP]-
Autor(es): dc.creatorSalomão, Rondinelle Artur Simões-
Autor(es): dc.creatorMareco, Edson Assunção-
Autor(es): dc.creatorCarvalho, Robson Francisco [UNESP]-
Autor(es): dc.creatorde Paula, Tassiana Gutierrez [UNESP]-
Autor(es): dc.creatorBarros, Margarida Maria [UNESP]-
Autor(es): dc.creatorDal-Pai-silva, Maeli [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:50:16Z-
Data de disponibilização: dc.date.available2022-02-22T00:50:16Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-03-02-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/ijms22062995-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/207447-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/207447-
Descrição: dc.descriptionIn fish, fasting leads to loss of muscle mass. This condition triggers oxidative stress, and therefore, antioxidants can be an alternative to muscle recovery. We investigated the effects of antioxidant ascorbic acid (AA) on the morphology, antioxidant enzyme activity, and gene expression in the skeletal muscle of pacu (Piaractus mesopotamicus) following fasting, using in vitro and in vivo strategies. Isolated muscle cells of the pacu were subjected to 72 h of nutrient restriction, followed by 24 h of incubation with nutrients or nutrients and AA (200 µM). Fish were fasted for 15 days, followed by 6 h and 15 and 30 days of refeeding with 100, 200, and 400 mg/kg of AA supplementation. AA addition increased cell diameter and the expression of anabolic and cell proliferation genes in vitro. In vivo, 400 mg/kg of AA increased anabolic and proliferative genes expression at 6 h of refeeding, the fiber diameter and the expression of genes related to cell proliferation at 15 days, and the expression of catabolic and oxidative metabolism genes at 30 days. Catalase activity remained low in the higher supplementation group. In conclusion, AA directly affected the isolated muscle cells, and the higher AA supplementation positively influenced muscle growth after fasting.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionDepartment of Structural and Functional Biology Institute of Biosciences São Paulo State University UNESP-
Descrição: dc.descriptionDepartment of Histology Embryology and Cell Biology Institute of Biological Sciences Federal University of Goiás (UFG)-
Descrição: dc.descriptionDepartment of Breeding and Animal Nutrition School of Veterinary Medicine and Animal Science São Paulo State University UNESP-
Descrição: dc.descriptionEnvironment and Regional Development Graduate University of Western São Paulo-
Descrição: dc.descriptionDepartment of Structural and Functional Biology Institute of Biosciences São Paulo State University UNESP-
Descrição: dc.descriptionDepartment of Breeding and Animal Nutrition School of Veterinary Medicine and Animal Science São Paulo State University UNESP-
Descrição: dc.descriptionCAPES: 001-
Descrição: dc.descriptionFAPESP: 2017/26346-1-
Descrição: dc.descriptionFAPESP: 2019/06708-1-
Descrição: dc.descriptionCNPq: 307138/2018-6-
Descrição: dc.descriptionCNPq: 800436/2018-0-
Formato: dc.format1-19-
Idioma: dc.languageen-
Relação: dc.relationInternational Journal of Molecular Sciences-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAnabolism-
Palavras-chave: dc.subjectAscorbic acid-
Palavras-chave: dc.subjectFasting-
Palavras-chave: dc.subjectMyogenesis-
Palavras-chave: dc.subjectSkeletal muscle growth-
Título: dc.titleAscorbic acid supplementation improves skeletal muscle growth in pacu (Piaractus mesopotamicus) juveniles: In vivo and in vitro studies-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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