A Long-Term Conserved Satellite DNA That Remains Unexpanded in Several Genomes of Characiformes Fish Is Actively Transcribed

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Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorUppsala University-
Autor(es): dc.contributorRio de Janerio-
Autor(es): dc.creatorDos Santos, Rodrigo Zeni [UNESP]-
Autor(es): dc.creatorCalegari, Rodrigo Milan [UNESP]-
Autor(es): dc.creatorSilva, Duílio Mazzoni Zerbinato de Andrade [UNESP]-
Autor(es): dc.creatorRuiz-Ruano, Francisco J.-
Autor(es): dc.creatorMelo, Silvana [UNESP]-
Autor(es): dc.creatorOliveira, Claudio [UNESP]-
Autor(es): dc.creatorForesti, Fausto [UNESP]-
Autor(es): dc.creatorUliano-Silva, Marcela-
Autor(es): dc.creatorPorto-Foresti, Fábio [UNESP]-
Autor(es): dc.creatorUtsunomia, Ricardo [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:50:10Z-
Data de disponibilização: dc.date.available2022-02-22T00:50:10Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-02-02-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1093/gbe/evab002-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/207410-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/207410-
Descrição: dc.descriptionEukaryotic genomes contain large amounts of repetitive DNA sequences, such as tandemly repeated satellite DNAs (satDNAs). These sequences are highly dynamic and tend to be genus- or species-specific due to their particular evolutionary pathways, although there are few unusual cases of conserved satDNAs over long periods of time. Here, we used multiple approaches to reveal that an satDNA named CharSat01-52 originated in the last common ancestor of Characoidei fish, a superfamily within the Characiformes order, ∼140-78 Ma, whereas its nucleotide composition has remained considerably conserved in several taxa. We show that 14 distantly related species within Characoidei share the presence of this satDNA, which is highly amplified and clustered in subtelomeric regions in a single species (Characidium gomesi), while remained organized as small clusters in all the other species. Defying predictions of the molecular drive of satellite evolution, CharSat01-52 shows similar values of intra- and interspecific divergence. Although we did not provide evidence for a specific functional role of CharSat01-52, its transcriptional activity was demonstrated in different species. In addition, we identified short tandem arrays of CharSat01-52 embedded within single-molecule real-time long reads of Astyanax paranae (536 bp-3.1 kb) and A. mexicanus (501 bp-3.9 kb). Such arrays consisted of head-to-tail repeats and could be found interspersed with other sequences, inverted sequences, or neighbored by other satellites. Our results provide a detailed characterization of an old and conserved satDNA, challenging general predictions of satDNA evolution.-
Descrição: dc.descriptionDepartamento de Ciências Biológicas Faculdade de Ciências Universidade Estadual Paulista UNESP Campus de Bauru-
Descrição: dc.descriptionDepartamento de Biologia Estrutural e Funcional Instituto de Biociências de Botucatu Universidade Estadual Paulista UNESP-
Descrição: dc.descriptionDepartment of Organismal Biology-Systematic Biology Evolutionary Biology Centre Uppsala University-
Descrição: dc.descriptionDepartamento de Genética Instituto de Ciências Biológicas e da Saúde ICBS Universidade Federal Rural do Rio de Janeiro Rio de Janerio-
Descrição: dc.descriptionDepartamento de Ciências Biológicas Faculdade de Ciências Universidade Estadual Paulista UNESP Campus de Bauru-
Descrição: dc.descriptionDepartamento de Biologia Estrutural e Funcional Instituto de Biociências de Botucatu Universidade Estadual Paulista UNESP-
Idioma: dc.languageen-
Relação: dc.relationGenome biology and evolution-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectneotropical fish-
Palavras-chave: dc.subjectrepetitive DNA-
Palavras-chave: dc.subjectsatDNA-
Palavras-chave: dc.subjecttandem repeats-
Título: dc.titleA Long-Term Conserved Satellite DNA That Remains Unexpanded in Several Genomes of Characiformes Fish Is Actively Transcribed-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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