Preparation and characterization of leukocyte- and platelet-rich fibrin membrane derived from cats' blood

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.contributorFACOPH School—Natal-
Autor(es): dc.contributorAnhanguera University-
Autor(es): dc.creatorCastilho, Maíra Sales [UNESP]-
Autor(es): dc.creatorRahal, Sheila Canevese [UNESP]-
Autor(es): dc.creatorDias Neto, Ramiro das Neves [UNESP]-
Autor(es): dc.creatorPereira, Ana Cristina-
Autor(es): dc.creatorFrancia, Camila Contin Diniz de Almeida [UNESP]-
Autor(es): dc.creatorMesquita, Luciane dos Reis [UNESP]-
Autor(es): dc.creatorAntunes, Carina Bueno-
Autor(es): dc.creatorLainetti, Patrícia de Faria [UNESP]-
Autor(es): dc.creatorFonseca-Alves, Carlos Eduardo [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:50:05Z-
Data de disponibilização: dc.date.available2022-02-22T00:50:05Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2020-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1002/jemt.23737-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/207381-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/207381-
Descrição: dc.descriptionAutologous platelet concentrates have been used in regenerative medicine in humans due to the abundance of growth factors, but there are only a few reports in small animals. This study aimed to prepare and characterize a leukocyte and platelet-rich fibrin membrane (L-PRF) produced with blood obtained from cats. Thirteen client-owned healthy adult Maine Coon cats were enrolled. The blood samples were collected and centrifuged at 650g for 12 min using a centrifuge specifically designed for this application. The L-PRF clot was removed from the tube and red blood cell base layer was separated, leaving buffy coat intact. After this, L-PRF clot was compressed by specialized metal plate for 30–60 s, and L-PRF membrane was obtained. Light microscopy examination of the membranes showed three distinct layers: white part, buffy coat, and red part. Immunohistochemical analysis demonstrated expression of vascular endothelial growth factor and platelet derived growth factor. The scanning electron microscopy showed that three-dimensional architecture of fibrin network was more compact in the area near the buffy coat. In conclusion, the method used allowed the characterization of the L-PRF membrane composition, which presented cell types and fibrin network architecture similar to those described in the human species.-
Descrição: dc.descriptionDepartment of Veterinary Surgery and Animal Reproduction School of Veterinary Medicine and Animal Science—São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Dentistry FACOPH School—Natal-
Descrição: dc.descriptionDepartment of Anatomy Institute of Biosciences—São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Veterinary Medicine Anhanguera University-
Descrição: dc.descriptionDepartment of Veterinary Surgery and Animal Reproduction School of Veterinary Medicine and Animal Science—São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Anatomy Institute of Biosciences—São Paulo State University (UNESP)-
Idioma: dc.languageen-
Relação: dc.relationMicroscopy Research and Technique-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectelectron microscopy-
Palavras-chave: dc.subjectfibrin membrane-
Palavras-chave: dc.subjecthistology-
Palavras-chave: dc.subjectimmunohistochemical-
Palavras-chave: dc.subjectL-PRF-
Palavras-chave: dc.subjectplatelet-
Título: dc.titlePreparation and characterization of leukocyte- and platelet-rich fibrin membrane derived from cats' blood-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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