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Metadados | Descrição | Idioma |
---|---|---|
Autor(es): dc.contributor | Universidade Estadual Paulista (Unesp) | - |
Autor(es): dc.contributor | FACOPH School—Natal | - |
Autor(es): dc.contributor | Anhanguera University | - |
Autor(es): dc.creator | Castilho, Maíra Sales [UNESP] | - |
Autor(es): dc.creator | Rahal, Sheila Canevese [UNESP] | - |
Autor(es): dc.creator | Dias Neto, Ramiro das Neves [UNESP] | - |
Autor(es): dc.creator | Pereira, Ana Cristina | - |
Autor(es): dc.creator | Francia, Camila Contin Diniz de Almeida [UNESP] | - |
Autor(es): dc.creator | Mesquita, Luciane dos Reis [UNESP] | - |
Autor(es): dc.creator | Antunes, Carina Bueno | - |
Autor(es): dc.creator | Lainetti, Patrícia de Faria [UNESP] | - |
Autor(es): dc.creator | Fonseca-Alves, Carlos Eduardo [UNESP] | - |
Data de aceite: dc.date.accessioned | 2022-02-22T00:50:05Z | - |
Data de disponibilização: dc.date.available | 2022-02-22T00:50:05Z | - |
Data de envio: dc.date.issued | 2021-06-25 | - |
Data de envio: dc.date.issued | 2021-06-25 | - |
Data de envio: dc.date.issued | 2020-12-31 | - |
Fonte completa do material: dc.identifier | http://dx.doi.org/10.1002/jemt.23737 | - |
Fonte completa do material: dc.identifier | http://hdl.handle.net/11449/207381 | - |
Fonte: dc.identifier.uri | http://educapes.capes.gov.br/handle/11449/207381 | - |
Descrição: dc.description | Autologous platelet concentrates have been used in regenerative medicine in humans due to the abundance of growth factors, but there are only a few reports in small animals. This study aimed to prepare and characterize a leukocyte and platelet-rich fibrin membrane (L-PRF) produced with blood obtained from cats. Thirteen client-owned healthy adult Maine Coon cats were enrolled. The blood samples were collected and centrifuged at 650g for 12 min using a centrifuge specifically designed for this application. The L-PRF clot was removed from the tube and red blood cell base layer was separated, leaving buffy coat intact. After this, L-PRF clot was compressed by specialized metal plate for 30–60 s, and L-PRF membrane was obtained. Light microscopy examination of the membranes showed three distinct layers: white part, buffy coat, and red part. Immunohistochemical analysis demonstrated expression of vascular endothelial growth factor and platelet derived growth factor. The scanning electron microscopy showed that three-dimensional architecture of fibrin network was more compact in the area near the buffy coat. In conclusion, the method used allowed the characterization of the L-PRF membrane composition, which presented cell types and fibrin network architecture similar to those described in the human species. | - |
Descrição: dc.description | Department of Veterinary Surgery and Animal Reproduction School of Veterinary Medicine and Animal Science—São Paulo State University (UNESP) | - |
Descrição: dc.description | Department of Dentistry FACOPH School—Natal | - |
Descrição: dc.description | Department of Anatomy Institute of Biosciences—São Paulo State University (UNESP) | - |
Descrição: dc.description | Department of Veterinary Medicine Anhanguera University | - |
Descrição: dc.description | Department of Veterinary Surgery and Animal Reproduction School of Veterinary Medicine and Animal Science—São Paulo State University (UNESP) | - |
Descrição: dc.description | Department of Anatomy Institute of Biosciences—São Paulo State University (UNESP) | - |
Idioma: dc.language | en | - |
Relação: dc.relation | Microscopy Research and Technique | - |
???dc.source???: dc.source | Scopus | - |
Palavras-chave: dc.subject | electron microscopy | - |
Palavras-chave: dc.subject | fibrin membrane | - |
Palavras-chave: dc.subject | histology | - |
Palavras-chave: dc.subject | immunohistochemical | - |
Palavras-chave: dc.subject | L-PRF | - |
Palavras-chave: dc.subject | platelet | - |
Título: dc.title | Preparation and characterization of leukocyte- and platelet-rich fibrin membrane derived from cats' blood | - |
Tipo de arquivo: dc.type | livro digital | - |
Aparece nas coleções: | Repositório Institucional - Unesp |
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