Chalcone T4, a novel chalconic compound, inhibits inflammatory bone resorption in vivo and suppresses osteoclastogenesis in vitro

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.creatorFernandes, Natalie Aparecida Rodrigues [UNESP]-
Autor(es): dc.creatorCamilli, Angelo Constantino [UNESP]-
Autor(es): dc.creatorMaldonado, Laura Andrea Gonzalez [UNESP]-
Autor(es): dc.creatorPacheco, Cindy Grace Pérez [UNESP]-
Autor(es): dc.creatorSilva, Amanda Favoreto [UNESP]-
Autor(es): dc.creatorMolon, Rafael Scaf [UNESP]-
Autor(es): dc.creatorSpolidorio, Luiz Carlos [UNESP]-
Autor(es): dc.creatorRibeiro de Assis, Letícia [UNESP]-
Autor(es): dc.creatorRegasini, Luis Octavio [UNESP]-
Autor(es): dc.creatorRossa Junior, Carlos [UNESP]-
Autor(es): dc.creatorGuimarães-Stabili, Morgana Rodrigues [UNESP]-
Data de aceite: dc.date.accessioned2022-02-22T00:50:02Z-
Data de disponibilização: dc.date.available2022-02-22T00:50:02Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1111/jre.12857-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/207375-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/207375-
Descrição: dc.descriptionObjective: This study aimed to assess the effect of a novel synthetic chalcone, Chalcone T4, on a murine model of periodontitis and on RANKL-induced osteoclastogenesis in vitro. Background: Chalcones are natural compounds with anti-inflammatory properties, and its synthetic analogs with enhanced biological effects have potential as therapeutic agents. Periodontitis is characterized by chronic inflammation of the periodontium and alveolar bone resorption. Safe and effective anti-inflammatory agents can have an important additive effect in the treatment in this disease. Methods: Periodontitis was induced via the installation of a ligature around the first molar. Rats (n = 32) received Chalcone T4 (5 and 50 mg/kg) or distilled water by gavage daily for 15 days. Outcomes assessed were bone resorption (μCT), TNF-α production (ELISA), cellular infiltrate, and collagen content (stereometric analysis, CD45+ cells by immunohistochemistry), and activation of NFATc1 and NF-kB (immunohistochemistry). In vitro, RAW 264.7 were treated with Chalcone T4 and stimulated with RANKL for assessment of osteoclast differentiation (actin ring staining) and activity (pit assay). Results: Chalcone T4 significantly reduced periodontitis-associated bone resorption, as well as the cellular infiltrate, while increasing the collagen content. Production of TNF-α, infiltration of CD45-positive cells, and NF-kB activation were markedly reduced. In vitro, chalcone T4 inhibited both osteoclast differentiation and activity. Conclusion: Chalcone T4 significantly inhibited alveolar bone resorption and inflammation in vivo and RANKL-induced osteoclastogenesis in vitro, suggesting a therapeutic role for this compound in the treatment of periodontitis.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionDepartment of Diagnosis and Surgery School of Dentistry at Araraquara São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Physiology and Pathology School of Dentistry at Araraquara São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Chemistry and Environmental Sciences São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Diagnosis and Surgery School of Dentistry at Araraquara São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Physiology and Pathology School of Dentistry at Araraquara São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Chemistry and Environmental Sciences São Paulo State University (UNESP)-
Descrição: dc.descriptionFAPESP: 2018/17047-3-
Formato: dc.format569-578-
Idioma: dc.languageen-
Relação: dc.relationJournal of Periodontal Research-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectbone resorption-
Palavras-chave: dc.subjectchalcone-
Palavras-chave: dc.subjectinflammation-
Palavras-chave: dc.subjectperiodontitis-
Título: dc.titleChalcone T4, a novel chalconic compound, inhibits inflammatory bone resorption in vivo and suppresses osteoclastogenesis in vitro-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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