HydroGEV: Extracellular Vesicle-Laden Hydrogel for Wound Healing Applications

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorThe University of Sydney-
Autor(es): dc.contributorUniversity of Technology Sydney-
Autor(es): dc.contributorUniversity of Melbourne-
Autor(es): dc.contributorRoyal Women’s Hospital-
Autor(es): dc.contributorAjou University-
Autor(es): dc.contributorUniversidade Estadual Paulista (Unesp)-
Autor(es): dc.creatorLei, Qingyu-
Autor(es): dc.creatorPhan, Thanh Huyen-
Autor(es): dc.creatorLe Thi, Phuong-
Autor(es): dc.creatorPoon, Christine-
Autor(es): dc.creatorPansani, Taisa Nogueira [UNESP]-
Autor(es): dc.creatorKabakowa, Irina-
Autor(es): dc.creatorKalionis, Bill-
Autor(es): dc.creatorPark, Ki Dong-
Autor(es): dc.creatorChrzanowski, Wojciech-
Data de aceite: dc.date.accessioned2022-02-22T00:49:55Z-
Data de disponibilização: dc.date.available2022-02-22T00:49:55Z-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2021-06-25-
Data de envio: dc.date.issued2020-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1007/978-3-030-62045-5_8-
Fonte completa do material: dc.identifierhttp://hdl.handle.net/11449/207335-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/207335-
Descrição: dc.descriptionChronic wounds contribute a substantial social and economic burden on the healthcare system. The global cost of wound treatment was about $19.8 Billion USD in 2019. Healing of chronic wounds takes typically more than 3 months. Current treatments are ineffective and do not always promote wound closure, which requires the activation of multiple cell types. Extracellular vesicles (EVs) contain multiple biomolecules that influence surrounding cells and thus have large capacity to promote tissue repair. To harness the chemoattractant properties of EVs, we developed an extracellular vesicle-laden hydrogel (HydroGEV) with optimized stiffness to promote functional tissue repair, since both mechanical and biological factors influence cell growth and subsequent tissue repair. EVs were isolated and purified from placental stem cells, characterized and incorporated into a gelatin-based hydrogel (GHPA) with different relative stiff-nesses (low, medium and high) determined by crosslinking density. The EVs were found to increase the migration capability of cells in a migration assay, confirming their strong chemoattractant properties and supporting their application for cell recruitment in wound healing. When incorporated into GHPA hydrogels, the EVs effectively improved cell attachment regardless of the stiffness of the hydrogels. Importantly, we demonstrated that by optimizing hydrogel stiffness it was possible to achieve higher cell proliferation and more phenotypic morphology. These promising results support the potential of HydroGEV as a better therapeutic option for patients with acute or chronic wounds.-
Descrição: dc.descriptionFaculty of Medicine and Health Sydney School of Pharmacy Sydney Nano Institute The University of Sydney-
Descrição: dc.descriptionFaculty of Science School of Mathematical and Physical Sciences University of Technology Sydney-
Descrição: dc.descriptionFaculty of Engineering and IT School of Biomedical Engineering University of Technology Sydney-
Descrição: dc.descriptionDepartment of Maternal-Fetal Medicine Pregnancy Research Centre University of Melbourne-
Descrição: dc.descriptionDepartment of Obstetrics and Gynaecology Royal Women’s Hospital-
Descrição: dc.descriptionDepartment of Molecular Science and Technology Ajou University-
Descrição: dc.descriptionDepartment of Dental Materials and Prosthodontics Araraquara School of Dentistry UNESP–University Estadual Paulista-
Descrição: dc.descriptionDepartment of Dental Materials and Prosthodontics Araraquara School of Dentistry UNESP–University Estadual Paulista-
Formato: dc.format81-89-
Idioma: dc.languageen-
Relação: dc.relationIFMBE Proceedings-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectExtracellular vesicles-
Palavras-chave: dc.subjectHydrogel-
Palavras-chave: dc.subjectNanomedicine-
Palavras-chave: dc.subjectRegenerative medicine-
Palavras-chave: dc.subjectStem cells-
Palavras-chave: dc.subjectTissue engineering-
Palavras-chave: dc.subjectWound healing-
Título: dc.titleHydroGEV: Extracellular Vesicle-Laden Hydrogel for Wound Healing Applications-
Aparece nas coleções:Repositório Institucional - Unesp

Não existem arquivos associados a este item.